ARHGEF7 (NM_003899) Human Tagged ORF Clone
CAT#: RG218946
- TrueORF®
ARHGEF7 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1
ORF Plasmid: DDK
"NM_003899" in other vectors (6)
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CNY 6,408.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BETA-PIX; COOL-1; COOL1; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PIXB |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG218946 representing NM_003899
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGATAATAGCAACAATCAACTGGTAGTAAGAGCAAAGTTTAACTTCCAGCAGACCAATGAGGACG AGCTTTCCTTCTCAAAAGGAGACGTCATCCATGTCACCCGTGTGGAAGAGGGAGGCTGGTGGGAGGGCAC ACTCAACGGCCGGACCGGCTGGTTCCCCAGCAACTACGTGCGCGAGGTCAAGGCCAGCGAGAAGCCTGTG TCTCCCAAATCAGGAACACTGAAGAGCCCTCCCAAAGGATTTGATACGACTGCCATAAACAAAAGCTATT ACAATGTGGTGCTACAGAATATTTTAGAAACAGAAAATGAATATTCTAAAGAACTTCAGACTGTGCTTTC AACGTACCTACGGCCATTGCAGACCAGTGAGAAGTTAAGTTCAGCAAACATTTCATATTTAATGGGAAAT CTAGAAGAAATATGTTCTTTCCAGCAAATGCTCGTACAGTCTTTAGAAGAATGCACCAAGTTGCCCGAAG CTCAGCAGAGAGTCGGAGGCTGCTTTTTAAACCTGATGCCACAGATGAAAACCCTGTACCTCACGTATTG TGCCAATCACCCTTCTGCAGTGAATGTCCTCACGGAACACAGTGAGGAGTTGGGGGAGTTCATGGAGACC AAAGGTGCCAGCAGCCCTGGGATTCTCGTGCTGACCACGGGCCTGAGCAAACCCTTCATGCGCCTGGATA AATACCCTACGCTGCTCAAAGAGCTCGAGAGACACATGGAGGATTATCATACAGATAGACAAGATATTCA AAAATCCATGGCTGCCTTCAAAAACCTTTCAGCCCAATGTCAAGAAGTCCGGAAGAGGAAAGAGCTTGAG CTGCAGATCCTGACGGAAGCCATCCGGAACTGGGAGGGCGATGACATTAAAACTCTGGGCAACGTCACTT ACATGTCCCAGGTCCTGATTCAGTGTGCCGGAAGTGAGGAAAAGAATGAAAGATATCTTCTACTCTTCCC AAATGTTTTGCTAATGTTGTCTGCCAGTCCTAGGATGAGTGGCTTTATCTATCAGGGAAAGCTTCCAACG ACAGGAATGACAATCACAAAGCTTGAGGACAGTGAAAATCATAGAAATGCATTTGAAATATCAGGGAGCA TGATTGAGCGGATATTAGTGTCGTGCAACAACCAGCAGGATCTGCAGGAATGGGTGGAGCACCTACAGAA GCAAACGAAGGTCACGTCTGTGGGAAACCCCACCATAAAGCCTCATTCAGTGCCATCTCATACCCTCCCC TCCCACCCGGTCACTCCGTCCAGCAAGCACGCAGACAGCAAGCCCGCGCCGCTGACGCCCGCCTACCACA CGCTGCCCCACCCCTCCCACCACGGCACCCCGCACACCACCATCAACTGGGGACCCCTGGAGCCTCCGAA AACACCCAAGCCCTGGAGCCTGAGCTGCCTGCGGCCCGCGCCTCCCCTCCGGCCCTCAGCTGCTCTCTGC TACAAGGAGGATCTTAGTAAGAGCCCTAAGACCATGAAAAAGCTGCTGCCCAAGCGCAAACCTGAACGGA AGCCTTCAGATGAGGAGTTCGCGTCCCGGAAAAGCACAGCTGCTTTGGAAGAAGATGCTCAGATTCTGAA AGTCATTGAAGCTTACTGCACCAGCGCCAAAACAAGGCAAACACTCAATTCAAGTTCACGCAAAGAATCT GCTCCACAAGTTTTGCTTCCAGAAGAAGAGAAAATTATAGTGGAAGAAACTAAAAGTAATGGTCAGACAG TGATAGAAGAAAAGAGTCTTGTGGATACCGTATATGCATTAAAGGATGAAGTTCAAGAATTAAGACAGGA CAACAAAAAGATGAAGAAATCTCTAGAGGAAGAACAGAGAGCCCGCAAAGACCTGGAGAAGCTGGTGAGG AAAGTCCTGAAGAACATGAATGATCCTGCCTGGGATGAGACCAATCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218946 representing NM_003899
Red=Cloning site Green=Tags(s) MTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPV SPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGN LEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMET KGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHTDRQDIQKSMAAFKNLSAQCQEVRKRKELE LQILTEAIRNWEGDDIKTLGNVTYMSQVLIQCAGSEEKNERYLLLFPNVLLMLSASPRMSGFIYQGKLPT TGMTITKLEDSENHRNAFEISGSMIERILVSCNNQQDLQEWVEHLQKQTKVTSVGNPTIKPHSVPSHTLP SHPVTPSSKHADSKPAPLTPAYHTLPHPSHHGTPHTTINWGPLEPPKTPKPWSLSCLRPAPPLRPSAALC YKEDLSKSPKTMKKLLPKRKPERKPSDEEFASRKSTAALEEDAQILKVIEAYCTSAKTRQTLNSSSRKES APQVLLPEEEKIIVEETKSNGQTVIEEKSLVDTVYALKDEVQELRQDNKKMKKSLEEEQRARKDLEKLVR KVLKNMNDPAWDETNL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_003899 |
ORF Size | 1938 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003899.5 |
RefSeq Size | 5032 bp |
RefSeq ORF | 1941 bp |
Locus ID | 8874 |
UniProt ID | Q14155 |
Domains | RhoGEF, SH3, PH |
Protein Pathways | Regulation of actin cytoskeleton |
Gene Summary | This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Mar 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218946 | ARHGEF7 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1 |
CNY 4,808.00 |
|
RC218946L1 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, Myc-DDK-tagged |
CNY 7,208.00 |
|
RC218946L2 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
RC218946L3 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC218946L4 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
SC117668 | ARHGEF7 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 7 (ARHGEF7), transcript variant 1 |
CNY 4,816.00 |
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