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SIM2 (NM_005069) Human Tagged ORF Clone
CAT＃: RG217892
- TrueORF®
SIM2 (tGFP-tagged) - Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2
ORF Plasmid: DDK
"NM_005069" in other vectors (4)
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CNY 8,928.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bHLHe15; HMC13F06; HMC29C01; SIM |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217892 representing NM_005069
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGAAGTCCAAGAATGCGGCCAAGACCAGGAGGGAGAAGGAAAATGGCGAGTTTTACGAGCTTG CCAAGCTGCTCCCGCTGCCGTCGGCCATCACTTCGCAGCTGGACAAAGCGTCCATCATCCGCCTCACCAC GAGCTACCTGAAGATGCGCGCCGTCTTCCCCGAAGGTTTAGGAGACGCGTGGGGACAGCCGAGCCGCGCC GGGCCCCTGGACGGCGTCGCCAAGGAGCTGGGATCGCACTTGCTGCAGACTTTGGATGGATTTGTTTTTG TGGTAGCATCTGATGGCAAAATCATGTATATATCCGAGACCGCTTCTGTCCATTTAGGCTTATCCCAGGT GGAGCTCACGGGCAACAGTATTTATGAATACATCCATCCTTCTGACCACGATGAGATGACCGCTGTCCTC ACGGCCCACCAGCCGCTGCACCACCACCTGCTCCAAGAGTATGAGATAGAGAGGTCGTTCTTTCTTCGAA TGAAATGTGTCTTGGCGAAAAGGAACGCGGGCCTGACCTGCAGCGGATACAAGGTCATCCACTGCAGTGG CTACTTGAAGATCAGGCAGTATATGCTGGACATGTCCCTGTACGACTCCTGCTACCAGATTGTGGGGCTG GTGGCCGTGGGCCAGTCGCTGCCACCCAGTGCCATCACCGAGATCAAGCTGTACAGTAACATGTTCATGT TCAGGGCCAGCCTTGACCTGAAGCTGATATTCCTGGATTCCAGGGTGACCGAGGTGACGGGKTACGAGCC GCAGGACCTGATCGAGAAGACCCTATACCATCACGTGCACGGCTGCGACGTGTTCCACCTCCGCTACGCA CACCACCTCCTGTTGGTGAAGGGCCAGGTCACCACCAAGTACTACCGGCTGCTGTCCAAGCGGGGCGGCT GGGTGTGGGTGCAGAGCTACGCCACCGTGGTGCACAACAGCCGCTCGTCCCGGCCCCACTGCATCGTGAG TGTCAATTATGTACTCACGGAGATTGAATACAAGGAACTTCAGCTGTCCCTGGAGCAGGTGTCCACTGCC AAGTCCCAGGACTCCTGGAGGACCGCCTTGTCTACCTCACAAGAAACTAGGAAATTAGTGAAACCCAAAA ATACCAAGATGAAGACAAAGCTGAGAACAAACCCTTACCCCCCACAGCAATACAGCTCGTTCCAAATGGA CAAACTGGAATGCGGCCAGCTCGGAAACTGGAGAGCCAGTCCCCCTGCAAGCGCTGCTGCTCCTCCAGAA CTGCAGCCCCACTCAGAAAGCAGTGACCTTCTGTACACGCCATCCTACAGCCTGCCCTTCTCCTACCATT ACGGACACTTCCCTCTGGACTCTCACGTCTTCAGCAGCAAAAAGCCAATGTTGCCGGCCAAGTTCGGGCA GCCCCAAGGATCCCCTTGTGAGGTGGCACGCTTTTTCCTGAGCACAATGCCAGCCAGCGGTGAATGCCAG TGGCATTATGCCAACCCCCTAGTGCCTAGCAGCTCGTCTCCAGCTAAAAATCCTCCAGAGCCACCGGCGA ACACTGCTAGGCACAGCCTGGTGCCAAGCTACGAAGCGCCCGCCGCCGCCGTGCGCAGGTTCGGCGAGGA CACCGCGCCCCCGAGCTTCCCGAGCTGCGGCCACTACCGCGAGGAGCCCGCGCTGGGCCCGGCCAAAGCC GCCCGCCAGGCCGCCCGGGACGGGGCGCGGCTGGCGCTGGCCCGCGCGGCACCCGAGTGCTGCGCGCCCC CGACCCCCGAGGCCCCGGGCGCGCCGGCGCAGCTGCCCTTCGTGCTGCTCAACTACCACCGCGTGCTGGC CCGGCGCGGACCGCTGGGGGGCGCCGCACCCGCCGCCTCCGGCCTGGCCTGCGCTCCCGGCGGCCCCGAG GCGGCGACCGGCGCGCTGCGGCTCCGGCACCCGAGCCCCGCCGCCACCTCCCCGCCCGGCGCGCCCCTGC CGCACTACCTGGGCGCCTCGGTCATCATCACCAACGGGAGG ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217892 representing NM_005069
Red=Cloning site Green=Tags(s) MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGLGDAWGQPSRA GPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELTGNSIYEYIHPSDHDEMTAVL TAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGYKVIHCSGYLKIRQYMLDMSLYDSCYQIVGL VAVGQSLPPSAITEIKLYSNMFMFRASLDLKLIFLDSRVTEVTXYEPQDLIEKTLYHHVHGCDVFHLRYA HHLLLVKGQVTTKYYRLLSKRGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTA KSQDSWRTALSTSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPE LQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFLSTMPASGECQ WHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEAPAAAVRRFGEDTAPPSFPSCGHYREEPALGPAKA ARQAARDGARLALARAAPECCAPPTPEAPGAPAQLPFVLLNYHRVLARRGPLGGAAPAASGLACAPGGPE AATGALRLRHPSPAATSPPGAPLPHYLGASVIITNGR TRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005069 |
| ORF Size | 2001 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005069.2, NP_005060.1 |
| RefSeq Size | 3885 bp |
| RefSeq ORF | 2004 bp |
| Locus ID | 6493 |
| UniProt ID | Q14190 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene represents a homolog of the Drosophila single-minded (sim) gene, which encodes a transcription factor that is a master regulator of neurogenesis. The encoded protein is ubiquitinated by RING-IBR-RING-type E3 ubiquitin ligases, including the parkin RBR E3 ubiquitin protein ligase. This gene maps within the so-called Down syndrome chromosomal region, and is thus thought to contribute to some specific Down syndrome phenotypes. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CD24 and CK4 are upregulated by SIM2, and are predictive biomarkers for chemoradiotherapy and surgery in esophageal cancer
,Takashima, K;Fujii, S;Komatsuzaki, R;Komatsu, M;Takahashi, M;Kojima, T;Daiko, H;Minashi, K;Chiwaki, F;Muto, M;Sasaki, H;Yano, T;,
Int J Oncol
,PubMed ID 32124945
[SIM2]
|
|
Multiple roles of single-minded 2 in esophageal squamous cell carcinoma and its clinical implications
,Tamaoki, M;Komatsuzaki, R;Komatsu, M;Minashi, K;Aoyagi, K;Nishimura, T;Chiwaki, F;Hiroki, T;Daiko, H;Morishita, K;Sakai, Y;Seno, H;Chiba, T;Muto, M;Yoshida, T;Sasaki, H;,
Cancer Sci.
,PubMed ID 29427302
[SIM2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217892 | SIM2 (Myc-DDK-tagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2 |
CNY 5,800.00 |
|
| RC217892L3 | Lenti-ORF clone of SIM2 (Myc-DDK-tagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2 |
CNY 7,700.00 |
|
| RC217892L4 | Lenti-ORF clone of SIM2 (mGFP-tagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2 |
CNY 7,700.00 |
|
| SC303574 | SIM2 (untagged)-Human single-minded homolog 2 (Drosophila) (SIM2), transcript variant SIM2 |
CNY 7,336.00 |
