ADAMTS15 (NM_139055) Human Tagged ORF Clone
CAT#: RG217574
- TrueORF®
ADAMTS15 (tGFP-tagged) - Human ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15)
ORF Plasmid: DDK
"NM_139055" in other vectors (6)
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CNY 12,032.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217574 representing NM_139055
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCTGCTGGGCATCCTAACCCTGGCTTTCGCCGGGCGAACCGCTGGAGGCTCTGAGCCAGAGCGGG AGGTAGTCGTTCCCATCCGACTGGACCCGGACATTAACGGCCGCCGCTACTACTGGCGGGGTCCCGAGGA CTCCGGGGATCAGGGACTCATTTTTCAGATCACAGCATTTCAGGAGGACTTTTACCTACACCTGACGCCG GATGCTCAGTTCTTGGCTCCCGCCTTCTCCACTGAGCATCTGGGCGTCCCCCTCCAGGGGCTCACCGGGG GCTCTTCAGACCTGCGACGCTGCTTCTATTCTGGGGACGTGAACGCCGAGCCGGACTCGTTCGCTGCTGT GAGCCTGTGCGGGGGGCTCCGCGGAGCCTTTGGCTACCGAGGCGCCGAGTATGTCATTAGCCCGCTGCCC AATGCTAGCGCGCCGGCGGCGCAGCGCAACAGCCAGGGCGCACACCTTCTCCAGCGCCGGGGTGTTCCGG GCGGGCCTTCCGGAGACCCCACCTCTCGCTGCGGGGTGGCCTCGGGCTGGAACCCCGCCATCCTACGGGC CCTGGACCCTTACAAGCCGCGGCGGGCGGGCTTCGGGGAGAGTCGTAGCCGGCGCAGGTCTGGGCGCGCC AAGCGTTTCGTGTCTATCCCGCGGTACGTGGAGACGCTGGTGGTCGCGGACGAGTCAATGGTCAAGTTCC ACGGCGCGGACCTGGAACATTATCTGCTGACGCTGCTGGCAACGGCGGCGCGACTCTACCGCCATCCCAG CATCCTCAACCCCATCAACATCGTTGTGGTCAAGGTGCTGCTTCTTAGAGATCGTGACTCCGGGCCCAAG GTCACCGGCAATGCGGCCCTGACGCTGCGCAACTTCTGTGCCTGGCAGAAGAAGCTGAACAAAGTGAGTG ACAAGCACCCCGAGTACTGGGACACTGCCATCCTCTTCACCAGGCAGGACCTGTGTGGAGCCACCACCTG TGACACCCTGGGCATGGCTGATGTGGGTACCATGTGTGACCCCAAGAGAAGCTGCTCTGTCATTGAGGAC GATGGGCTTCCATCAGCCTTCACCACTGCCCACGAGCTGGGCCACGTGTTCAACATGCCCCATGACAATG TGAAAGTCTGTGAGGAGGTGTTTGGGAAGCTCCGAGCCAACCACATGATGTCCCCGACCCTCATCCAGAT CGACCGTGCCAACCCCTGGTCAGCCTGCAGTGCTGCCATCATCACCGACTTCCTGGACAGCGGGCACGGT GACTGCCTCCTGGACCAACCCAGCAAGCCCATCTCCCTGCCCGAGGATCTGCCGGGCGCCAGCTACACCC TGAGCCAGCAGTGCGAGCTGGCTTTTGGCGTGGGCTCCAAGCCCTGTCCTTACATGCAGTACTGCACCAA GCTGTGGTGCACCGGGAAGGCCAAGGGACAGATGGTGTGCCAGACCCGCCACTTCCCCTGGGCCGATGGC ACCAGCTGTGGCGAGGGCAAGCTCTGCCTCAAAGGGGCCTGCGTGGAGAGACACAACCTCAACAAGCACA GGGTGGATGGTTCCTGGGCCAAATGGGATCCCTATGGCCCCTGCTCGCGCACATGTGGTGGGGGCGTGCA GCTGGCCAGGAGGCAGTGCACCAACCCCACCCCTGCCAACGGGGGCAAGTACTGCGAGGGAGTGAGGGTG AAATACCGATCCTGCAATCTGGAGCCCTGCCCCAGCTCAGCCTCCGGAAAGAGCTTCCGGGAGGAGCAGT GTGAGGCTTTCAACGGCTACAACCACAGCACCAACCGGCTCACTCTCGCCGTGGCATGGGTGCCCAAGTA CTCCGGCGTGTCTCCCCGGGACAAGTGCAAGCTCATCTGCCGAGCCAATGGCACTGGCTACTTCTATGTG CTGGCACCCAAGGTGGTGGACGGCACGCTGTGCTCTCCTGACTCCACCTCCGTCTGTGTCCAAGGCAAGT GCATCAAGGCTGGCTGTGATGGGAACCTGGGCTCCAAGAAGAGATTCGACAAGTGTGGGGTGTGTGGGGG AGACAATAAGAGCTGCAAGAAGGTGACTGGACTCTTCACCAAGCCCATGCATGGCTACAATTTCGTGGTG GCCATCCCCGCAGGCGCCTCAAGCATCGACATCCGCCAGCGCGGTTACAAAGGGCTGATCGGGGATGACA ACTACCTGGCTCTGAAGAACAGCCAAGGCAAGTACCTGCTCAACGGGCATTTCGTGGTGTCGGCGGTGGA GCGGGACCTGGTGGTGAAGGGCAGTCTGCTGCGGTACAGCGGCACGGGCACAGCGGTGGAGAGCCTGCAG GCTTCCCGGCCCATCCTGGAGCCGCTGACCGTGGAGGTCCTCTCCGTGGGGAAGATGACACCGCCCCGGG TCCGCTACTCCTTCTATCTGCCCAAAGAGCCTCGGGAGGACAAGTCCTCTCATCCCAAGGACCCCCGGGG ACCCTCTGTCTTGCACAACAGCGTCCTCAGCCTCTCCAACCAGGTGGAGCAGCCGGACGACAGGCCCCCT GCACGCTGGGTGGCTGGCAGCTGGGGGCCGTGCTCCGCGAGCTGCGGCAGTGGCCTGCAGAAGCGGGCGG TGGACTGCCGGGGCTCCGCCGGGCAGCGCACGGTCCCTGCCTGTGATGCAGCCCATCGGCCCGTGGAGAC ACAAGCCTGCGGGGAGCCCTGCCCCACCTGGGAGCTCAGCGCCTGGTCACCCTGCTCCAAGAGCTGCGGC CGGGGATTTCAGAGGCGCTCACTCAAGTGTGTGGGCCACGGAGGCCGGCTGCTGGCCCGGGACCAGTGCA ACTTGCACCGCAAGCCCCAGGAGCTGGACTTCTGCGTCCTGAGGCCGTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217574 representing NM_139055
Red=Cloning site Green=Tags(s) MLLLGILTLAFAGRTAGGSEPEREVVVPIRLDPDINGRRYYWRGPEDSGDQGLIFQITAFQEDFYLHLTP DAQFLAPAFSTEHLGVPLQGLTGGSSDLRRCFYSGDVNAEPDSFAAVSLCGGLRGAFGYRGAEYVISPLP NASAPAAQRNSQGAHLLQRRGVPGGPSGDPTSRCGVASGWNPAILRALDPYKPRRAGFGESRSRRRSGRA KRFVSIPRYVETLVVADESMVKFHGADLEHYLLTLLATAARLYRHPSILNPINIVVVKVLLLRDRDSGPK VTGNAALTLRNFCAWQKKLNKVSDKHPEYWDTAILFTRQDLCGATTCDTLGMADVGTMCDPKRSCSVIED DGLPSAFTTAHELGHVFNMPHDNVKVCEEVFGKLRANHMMSPTLIQIDRANPWSACSAAIITDFLDSGHG DCLLDQPSKPISLPEDLPGASYTLSQQCELAFGVGSKPCPYMQYCTKLWCTGKAKGQMVCQTRHFPWADG TSCGEGKLCLKGACVERHNLNKHRVDGSWAKWDPYGPCSRTCGGGVQLARRQCTNPTPANGGKYCEGVRV KYRSCNLEPCPSSASGKSFREEQCEAFNGYNHSTNRLTLAVAWVPKYSGVSPRDKCKLICRANGTGYFYV LAPKVVDGTLCSPDSTSVCVQGKCIKAGCDGNLGSKKRFDKCGVCGGDNKSCKKVTGLFTKPMHGYNFVV AIPAGASSIDIRQRGYKGLIGDDNYLALKNSQGKYLLNGHFVVSAVERDLVVKGSLLRYSGTGTAVESLQ ASRPILEPLTVEVLSVGKMTPPRVRYSFYLPKEPREDKSSHPKDPRGPSVLHNSVLSLSNQVEQPDDRPP ARWVAGSWGPCSASCGSGLQKRAVDCRGSAGQRTVPACDAAHRPVETQACGEPCPTWELSAWSPCSKSCG RGFQRRSLKCVGHGGRLLARDQCNLHRKPQELDFCVLRPC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_139055 |
ORF Size | 2850 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_139055.3 |
RefSeq Size | 2853 bp |
RefSeq ORF | 2853 bp |
Locus ID | 170689 |
UniProt ID | Q8TE58 |
Protein Families | Druggable Genome, Protease, Secreted Protein |
Gene Summary | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme, which may play a role in versican processing during skeletal muscle development. This gene may function as a tumor suppressor in colorectal and breast cancers. [provided by RefSeq, May 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217574 | ADAMTS15 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15) |
CNY 10,432.00 |
|
RC217574L1 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15), Myc-DDK-tagged |
CNY 11,690.00 |
|
RC217574L2 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15), mGFP tagged |
CNY 12,832.00 |
|
RC217574L3 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15), Myc-DDK-tagged |
CNY 11,690.00 |
|
RC217574L4 | Lenti ORF clone of Human ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15), mGFP tagged |
CNY 11,690.00 |
|
SC125259 | ADAMTS15 (untagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 15 (ADAMTS15) |
CNY 18,340.00 |
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