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MED12 (NM_005120) Human Tagged ORF Clone
CAT＃: RG217206
- TrueORF®
MED12 (tGFP-tagged) - Human mediator complex subunit 12 (MED12)
ORF Plasmid: DDK
"NM_005120" in other vectors (3)
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CNY 23,160.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARC240; CAGH45; FGS1; HOPA; Kto; MED12S; OHDOX; OKS; OPA1; TNRC11; TRAP230 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217206 representing NM_005120
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAACAAAGTATGCCCTCTCTTCACACAAAAAAAATTCTATTCTGTTATTTTCACTTAACAAACAGCT GGTGCCTCCGGCGCTACGGGCTGGGCAAGATGGCGGCCTTCGGGATCTTGAGCTACGAACACCGGCCCCT GAAGCGGCCGCGGCCGCGGCTGGGGCCTCCCGATGTTTACCCTCAGGACCCCAAACAGAAGGAGGATGAA CTGACGGCCTTGAATGTAAAACAAGGTTTCAATAACCAGCCTGCTGTCTCTGGGGATGAGCATGGCAGTG CCAAGAACGTCAGCTTCAATCCTGCCAAGATCAGTTCCAACTTCAGCAGCATTATTGCAGAGAAATTACG TTGTAATACCCTTCCTGACACTGGTCGCAGGAAGCCCCAAGTGAACCAGAAGGATAACTTCTGGCTGGTG ACTGCACGATCCCAGAGTGCCATTAACACTTGGTTCACTGACTTGGCTGGCACCAAGCCACTCACGCAAC TAGCCAAAAAGGTCCCCATTTTCAGTAAGAAGGAAGAGGTGTTTGGGTACTTAGCCAAATACACAGTGCC TGTGATGCGGGCTGCCTGGCTCATTAAGATGACCTGTGCCTACTATGCAGCAATCTCTGAGACCAAGGTT AAGAAGAGACATGTTGACCCTTTCATGGAATGGACTCAGATCATCACCAAGTACTTATGGGAGCAGTTAC AGAAGATGGCTGAATACTACCGGCCAGGGCCTGCAGGAAGTGGGGGCTGTGGTTCCACGATAGGGCCCTT GCCCCATGATGTAGAGGTGGCAATCCGGCAGTGGGATTACACCGAGAAGCTGGCCATGTTCATGTTTCAG GATGGAATGCTGGACAGACATGAGTTCCTGACCTGGGTGCTTGAGTGTTTTGAGAAGATCCGCCCTGGAG AGGATGAATTGCTTAAACTGCTGCTGCCTCTGCTTCTCCGATACTCTGGGGAATTTGTTCAGTCTGCATA CCTGTCCCGCCGGCTTGCCTACTTCTGTACACGGAGACTGGCCCTGCAGCTGGATGGTGTGAGCAGTCAC TCATCTCATGTTATATCTGCTCAGTCAACAAGCACGCTACCCACCACCCCTGCTCCTCAGCCCCCAACTA GCAGCACACCCTCGACTCCCTTTAGTGACCTGCTTATGTGCCCTCAGCACCGGCCCCTGGTTTTTGGCCT CAGCTGTATCCTACAGACCATCCTCCTGTGCTGTCCTAGTGCCTTGGTTTGGCACTACTCACTGACTGAT AGCAGAATTAAGACCGGCTCACCACTTGACCACTTGCCTATTGCCCCGTCCAACCTGCCCATGCCAGAGG GTAACAGTGCCTTCACTCAGCAGGTCCGTGCAAAGTTGCGGGAGATCGAGCAGCAGATCAAGGAGCGGGG ACAGGCAGTTGAAGTTCGCTGGTCTTTCGATAAATGCCAGGAAGCTACTGCAGGCTTCACCATTGGACGG GTACTTCATACTTTGGAAGTGCTGGACAGCCATAGTTTTGAACGCTCTGACTTCAGCAACTCTCTTGACT CCCTTTGTAACCGAATCTTTGGATTGGGACCTAGCAAGGATGGGCATGAGATCTCCTCAGATGATGATGC TGTGGTGTCATTGCTATGTGAATGGGCTGTCAGCTGCAAGCGTTCTGGTCGGCATCGTGCTATGGTGGTA GCCAAGCTCCTGGAGAAGAGACAGGCGGAGATTGAGGCTGAGCGTTGTGGAGAATCAGAAGCCGCAGATG AGAAGGGTTCCATCGCCTCTGGCTCCCTTTCTGCTCCCAGTGCTCCCATTTTCCAGGATGTCCTCCTGCA GTTTCTGGATACACAGGCTCCCATGCTGACGGACCCTCGAAGTGAGAGTGAGCGGGTGGAATTCTTTAAC TTAGTACTGCTGTTCTGTGAACTGATTCGACATGATGTTTTCTCCCACAACATGTATACTTGCACTCTCA TCTCCCGAGGGGACCTTGCCTTTGGAGCCCCTGGTCCCCGGCCTCCCTCTCCCTTTGATGATCCTGCCGA TGACCCAGAGCACAAGGAGGCTGAAGGCAGCAGCAGCAGCAAGCTGGAAGATCCAGGGCTCTCAGAATCT ATGGACATTGACCCTAGTTCCAGTGTTCTCTTTGAGGACATGGAGAAGCCTGATTTCTCATTGTTCTCCC CTACTATGCCCTGTGAGGGGAAGGGCAGTCCATCCCCTGAGAAGCCAGATGTCGAGAAGGAGGTGAAGCC CCCACCCAAGGAGAAGATTGAAGGGACCCTTGGGGTTCTTTACGACCAGCCACGACACGTGCAGTACGCC ACCCATTTTCCCATCCCCCAGGAGGAGTCATGCAGCCATGAGTGCAACCAGCGGTTGGTCGTACTGTTTG GGGTGGGAAAGCAGCGAGATGATGCCCGCCATGCCATCAAGAAAATCACCAAGGATATCTTGAAGGTTCT GAACCGCAAAGGGACAGCAGAAACTGACCAGCTTGCTCCTATTGTGCCTCTGAATCCTGGAGACCTGACA TTCTTAGGTGGGGAGGATGGGCAGAAGCGGCGACGCAACCGGCCTGAAGCCTTCCCCACTGCTGAAGATA TCTTTGCTAAGTTCCAGCACCTTTCACATTATGACCAACACCAGGTCACGGCTCAGGTCTCCCGGAATGT TCTGGAGCAGATCACGAGCTTTGCCCTTGGCATGTCATACCACTTGCCTCTGGTGCAGCATGTGCAGTTC ATCTTCGACCTCATGGAATATTCACTCAGCATCAGTGGCCTCATCGACTTTGCCATTCAGCTGCTGAATG AACTGAGTGTAGTTGAGGCTGAGCTGCTTCTCAAATCCTCGGATCTGGTGGGCAGCTACACTACTAGCCT GTGCCTGTGCATCGTGGCTGTCCTGCGGCACTATCATGCCTGCCTCATCCTCAACCAGGACCAGATGGCA CAGGTCTTTGAGGGGCTGTGTGGCGTCGTGAAGCATGGGATGAACCGGTCCGATGGCTCCTCTGCAGAGC GCTGTATCCTTGCTTATCTCTATGATCTGTACACCTCCTGTAGCCATTTAAAGAACAAATTTGGGGAGCT CTTCAGCGACTTTTGCTCAAAGGTGAAGAACACCATCTACTGCAACGTGGAGCCATCGGAATCAAATATG CGCTGGGCACCTGAGTTCATGATCGACACTCTAGAGAACCCTGCAGCTCACACCTTCACCTACACGGGGC TAGGCAAGAGTCTTAGTGAGAACCCTGCTAACCGCTACAGCTTTGTCTGCAATGCCCTTATGCACGTCTG TGTGGGGCACCATGATCCCGATAGGGTGAATGACATCGCAATCCTGTGTGCAGAGCTGACCGGCTATTGC AAGTCACTGAGTGCAGAATGGCTAGGAGTGCTTAAGGCCTTGTGCTGCTCCTCTAACAATGGCACTTGTG GTTTCAACGATCTCCTCTGCAATGTTGATGTCAGTGACCTATCTTTTCATGACTCGCTGGCTACTTTTGT TGCCATCCTCATCGCTCGGCAGTGTTTGCTCCTGGAAGATCTGATTCGCTGTGCTGCCATCCCTTCACTC CTTAATGCTGCTTGTAGTGAACAGGACTCTGAGCCAGGGGCCCGGCTTACCTGCCGCATCCTCCTTCACC TTTTCAAGACACCGCAGCTCAATCCTTGCCAGTCTGATGGAAACAAGCCTACAGTAGGAATCCGCTCCTC CTGCGACCGCCACCTGCTGGCTGCCTCCCAGAACCGCATCGTGGATGGAGCCGTGTTTGCTGTTCTCAAG GCTGTGTTTGTACTTGGGGATGCGGAACTGAAAGGTTCAGGCTTCACTGTGACAGGAGGAACAGAAGAAC TTCCAGAGGAGGAGGGAGGAGGTGGCAGTGGTGGTCGGAGGCAGGGTGGCCGCAACATCTCTGTGGAGAC AGCCAGTCTGGATGTCTATGCCAAGTACGTGCTGCGCAGCATCTGCCAACAGGAATGGGTAGGAGAACGT TGCCTTAAGTCTCTGTGTGAGGACAGCAATGACCTGCAAGACCCAGTGTTGAGTAGTGCCCAGGCGCAGC GCCTCATGCAGCTCATTTGCTATCCACATCGACTGCTGGACAATGAGGATGGGGAAAACCCCCAGCGGCA GCGCATAAAGCGCATTCTCCAGAACTTGGACCAGTGGACCATGCGCCAGTCTTCCTTGGAGCTGCAGCTC ATGATCAAGCAGACCCCTAACAATGAGATGAACTCCCTCTTGGAGAACATCGCCAAGGCCACAATCGAGG TTTTCCAACGGTCAGCAGAGACAGGGTCATCTTCTGGAAGTACTGCAAGCAACATGCCCAGCAGCAGCAA GACCAAGCCTGTGCTCAGCTCTCTAGAGCGCTCTGGTGTATGGCTGGTGGCCCCCCTCATTGCTAAACTG CCCACCTCAGTCCAGGGACATGTGTTAAAGGCTGCTGGGGAAGAATTGGAGAAGGGTCAGCACCTGGGTT CCTCTTCACGCAAAGAACGTGATCGACAAAAGCAGAAGAGCATGTCCCTATTGAGCCAGCAGCCCTTCTT ATCGCTGGTGCTAACATGTCTGAAAGGGCAGGATGAACAACGCGAGGGACTCCTTACCTCCCTCTACAGC CAGGTGCACCAGATTGTGAATAATTGGCGAGATGACCAGTACTTAGATGATTGCAAACCAAAGCAGCTTA TGCATGAGGCACTCAAACTGCGGCTCAACCTGGTGGGGGGCATGTTTGACACGGTGCAGCGCAGCACCCA GCAGACCACGGAGTGGGCCATGCTCCTCCTGGAGATCATCATCAGCGGCACTGTCGACATGCAGTCCAAC AATGAGCTCTTCACTACTGTGTTGGACATGCTGAGCGTGCTCATCAATGGGACATTGGCTGCAGACATGT CTAGCATCTCGCAAGGTAGCATGGAGGAAAACAAGCGTGCATACATGAACCTGGCGAAGAAGTTGCAGAA GGAGTTGGGGGAGCGCCAGTCAGACAGTCTGGAAAAGGTTCGCCAGCTGCTGCCACTGCCCAAGCAGACC CGAGATGTCATCACGTGTGAGCCACAGGGCTCCCTTATCGATACCAAGGGCAACAAGATTGCTGGCTTCG ATTCCATCTTCAAGAAGGAGGGTCTACAGGTTTCCACCAAACAGAAGATCTCGCCCTGGGATCTTTTTGA GGGGTTGAAGCCGTCAGCACCACTCTCTTGGGGCTGGTTTGGAACAGTCCGAGTGGACCGGCGAGTGGCT CGAGGAGAGGAGCAGCAGCGGTTGCTGCTCTACCACACACACCTGAGGCCCCGGCCCCGCGCCTATTACC TGGAGCCACTGCCACTGCCCCCAGAAGATGAGGAGCCGCCTGCTCCTACCCTGCTAGAGCCTGAGAAAAA GGCTCCAGAGCCCCCCAAAACTGACAAACCGGGGGCTGCTCCACCCAGTACTGAGGAACGCAAGAAGAAG TCCACCAAGGGCAAGAAACGCAGCCAGCCAGCTACCAAGACAGAGGACTATGGAATGGGCCCGGGTCGGA GCGGCCCTTATGGTGTGACAGTGCCTCCGGACCTCCTGCACCACCCAAACCCTGGTTCTATAACACACCT TAACTACAGGCAAGGCTCCATAGGCCTGTACACCCAGAACCAGCCACTACCTGCAGGTGGCCCTCGTGTG GACCCATACCGTCCTGTGCGCTTACCAATGCAGAAGCTGCCCACCCGACCAACTTACCCTGGAGTGCTGC CCACAACCATGACTGGCGTCATGGGTTTAGAACCCTCCTCTTATAAGACCTCTGTGTACCGGCAGCAGCA ACCTGCGGTGCCCCAAGGACAGCGCCTTCGCCAACAGCTCCAGCAGAGTCAGGGCATGTTGGGACAGTCA TCTGTCCATCAGATGACTCCCAGCTCTTCCTACGGTTTGCAGACTTCCCAGGGCTATACTCCTTATGTTT CTCATGTGGGATTGCAGCAACACACAGGCCCTGCAGGTACCATGGTGCCCCCCAGCTACTCCAGCCAGCC TTACCAGAGCACCCACCCTTCTACCAATCCTACTCTTGTAGATCCTACCCGCCACCTGCAACAGCGGCCC AGTGGCTATGTGCACCAGCAGGCCCCCACCTATGGACATGGACTGACCTCCACTCAAAGGTTTTCACACC AGACACTGCAGCAGACACCCATGATAAGTACCATGACTCCAATGAGTGCCCAGGGCGTCCAGGCAGGCGT CCGTTCAACAGCCATCCTACCTGAGCAGCAGCAGCAGCAGCAACAGCAGCAACAGCAACAGCAGCAGCAG CAGCAACAGCAACAGCAGCAGCAGCAGCAGCAGTACCACATCCGGCAGCAGCAGCAGCAGCAGATCCTGC GGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAACAGCA ACAACAGCAACACCAGCAGCAACAGCAGCAACAGGCGGCTCCTCCCCAACCCCAGCCCCAGTCCCAGCCC CAGTTCCAGCGCCAGGGGCTTCAGCAGACCCAGCAGCAGCAACAGACAGCAGCTTTGGTCCGGCAACTTC AACAACAGCTCTCTAATACCCAGCCACAGCCCAGTACCAACATATTTGGACGCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217206 representing NM_005120
Red=Cloning site Green=Tags(s) MKQSMPSLHTKKILFCYFHLTNSWCLRRYGLGKMAAFGILSYEHRPLKRPRPRLGPPDVYPQDPKQKEDE LTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLV TARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKV KKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQ DGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSH SSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTD SRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGR VLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVV AKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFN LVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPEHKEAEGSSSSKLEDPGLSES MDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYA THFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLT FLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQF IFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMA QVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNM RWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYC KSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSL LNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLK AVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGER CLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQL MIKQTPNNEMNSLLENIAKATIEVFQRSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKL PTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYS QVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSN NELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQT RDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVA RGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKK STKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRV DPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQS SVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRP SGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQ QQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQP QFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005120 |
| ORF Size | 6636 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005120.1, NP_005111.1 |
| RefSeq Size | 6985 bp |
| RefSeq ORF | 6534 bp |
| Locus ID | 9968 |
| UniProt ID | Q93074 |
| Protein Families | Druggable Genome |
| Gene Summary | The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217206 | MED12 (Myc-DDK-tagged)-Human mediator complex subunit 12 (MED12) |
CNY 21,560.00 |
|
| RC217206L1 | Lenti ORF clone of Human mediator complex subunit 12 (MED12), Myc-DDK-tagged |
CNY 20,890.00 |
|
| SC303587 | MED12 (untagged)-Human mediator complex subunit 12 (MED12) |
CNY 41,990.00 |
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