CLASP1 (NM_015282) Human Tagged ORF Clone
CAT#: RG217019
- TrueORF®
CLASP1 (tGFP-tagged) - Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 1
ORF Plasmid: DDK
"NM_015282" in other vectors (4)
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CNY 16,820.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MAST1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217019 representing NM_015282
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTCGCATGGAGTCCTGCCTGGCGCAGGTGTTGCAGAAGGATGTGGGGAAACGATTGCAGGTTG GCCAAGAACTGATAGACTATTTCTCAGACAAACAGAAGTCTGCTGACCTTGAGCATGACCAGACCATGTT AGATAAACTTGTGGATGGACTTGCTACCTCTTGGGTGAACTCTAGCAATTACAAGGTGGTTCTGCTGGGC ATGGACATCCTGTCCGCCCTGGTGACCCGGCTGCAGGATCGGTTCAAGGCGCAGATCGGCACAGTGCTGC CAAGTCTAATAGACAGACTAGGAGATGCTAAAGACTCTGTGAGGGAGCAGGACCAAACTCTGCTGCTAAA GATCATGGATCAAGCTGCTAATCCCCAGTACGTATGGGACAGAATGCTTGGAGGCTTCAAACACAAGAAT TTCCGTACTCGAGAAGGCATCTGTCTCTGCCTTATAGCAACACTCAATGCCTCTGGAGCACAGACTTTAA CACTAAGCAAGATTGTGCCACATATATGCAACTTACTTGGAGATCCAAACAGCCAGGTTCGAGATGCAGC AATAAACAGCTTAGTGGAAATTTACAGACATGTAGGAGAACGTGTGAGGGCAGATCTCAGTAAAAAAGGA TTGCCACAGTCCCGGTTGAATGTAATTTTTACAAAATTTGATGAAGTCCAGAAATCTGGAAACATGATAC AATCTGCAAATGATAAAAATTTCGACGATGAAGATTCTGTGGATGGTAACAGACCTTCCTCTGCTAGTTC TACATCATCCAAGGCTCCACCAAGTTCTCGGAGAAACGTTGGAATGGGAACCACCCGCCGGCTTGGTTCA TCCACCCTTGGATCCAAGTCTTCAGCTGCAAAAGAAGGAGCTGGTGCTGTTGATGAAGAGGATTTTATTA AAGCATTTGATGATGTACCTGTAGTACAGATTTATTCCAGCCGAGACCTTGAGGAATCCATAAACAAAAT TAGGGAAATATTATCTGATGACAAGCATGATTGGGAGCAGAGAGTAAATGCTCTAAAAAAGATTAGATCT TTACTTTTGGCTGGTGCTGCTGAGTATGATAACTTCTTTCAACATTTGCGTCTTTTGGATGGAGCCTTTA AACTCTCTGCTAAGGACCTGCGGTCTCAGGTAGTGCGGGAGGCTTGTATCACGTTGGGGCATCTGTCATC AGTTCTGGGGAATAAGTTTGACCATGGAGCTGAAGCCATTATGCCAACTATCTTTAATTTAATTCCAAAC AGTGCCAAAATTATGGCCACATCTGGTGTTGTAGCTGTTAGGTTAATTATTCGGCACACACACATCCCTA GGTTAATACCTGTCATAACAAGCAACTGTACCTCTAAGTCTGTCGCAGTTAGAAGGCGCTGTTTTGAATT TTTAGATTTGCTTTTACAAGAATGGCAGACACATTCACTAGAACGACACATATCAGTATTAGCTGAAACA ATAAAGAAGGGAATACATGATGCTGATTCCGAAGCAAGAATAGAAGCCAGAAAATGTTACTGGGGTTTCC ACAGTCACTTCAGCAGAGAAGCAGAGCACTTGTACCACACCTTGGAGTCCTCCTACCAGAAAGCCCTGCA GTCCCACCTGAAGAACTCAGACAGCATAGTGTCTCTGCCTCAGTCAGACCGCTCATCTTCCAGCTCTCAA GAGAGTCTAAATCGTCCGCTGTCTGCCAAAAGAAGTCCTACTGGAAGTACCACATCTAGAGCTTCTACAG TTAGTACCAAATCTGTGTCAACGACTGGGTCCCTCCAGCGATCTCGAAGTGATATTGATGTGAACGCAGC AGCCAGTGCCAAATCCAAAGTCTCCTCATCTTCGGGCACGACGCCTTTCAGCTCTGCAGCAGCTTTGCCT CCAGGGTCATACGCATCCTTAGGTCGGATCCGCACAAGACGGCAAAGCTCTGGGAGTGCCACCAACGTCG CCTCTACACCTGATAACCGGGGCCGCAGTCGCGCTAAAGTGGTTTCACAGTCCCAGCGATCCAGATCTGC TAATCCTGCTGGTGCTGGCAGCCGGTCAAGTTCCCCAGGAAAATTGTTGGGAAGTGGTTATGGTGGACTT ACTGGGGGCTCCTCACGAGGCCCACCTGTGACACCGTCTTCAGAAAAGCGAAGCAAGATTCCCAGGAGCC AGGGATGTAGCCGGGAAACAAGTCCAAACCGAATAGGATTAGCACGGAGCAGCCGTATCCCTCGACCCAG CATGAGTCAGGGGTGCAGCCGCGATACCAGCCGTGAGAGCAGCCGAGATACAAGCCCTGCTCGGGGCTTT CCTCCACTTGATCGGTTTGGGCTTGGCCAGCCAGGAAGAATACCTGGTTCTGTGAATGCCATGAGAGTTC TGAGCACAAGTACAGATCTTGAAGCTGCTGTTGCTGATGCTTTGAAGAAGCCTGTGAGGAGGAGATATGA GCCGTATGGGATGTATTCTGACGATGATGCCAACAGTGATGCCTCAAGTGTTTGCTCTGAGCGCTCATAT GGCTCCAGGAATGGTGGCATTCCCCATTATCTGCGGCAGACTGAGGATGTAGCAGAAGTTCTCAACCACT GTGCTAGTTCAAACTGGTCAGAAAGGAAAGAAGGGCTTCTGGGCCTGCAGAACTTACTGAAGAGCCAAAG AACACTGAGTCGAGTTGAACTGAAAAGGTTGTGTGAGATCTTCACTCGGATGTTTGCTGACCCTCATAGC AAGAGAGTTTTCAGTATGTTTTTGGAGACTCTTGTGGATTTTATAATAATTCATAAGGATGATTTACAAG ACTGGCTTTTTGTTCTTCTCACACAATTACTTAAGAAAATGGGAGCAGATTTACTTGGATCTGTGCAAGC AAAAGTTCAAAAGGCTCTAGATGTCACAAGGGACTCCTTTCCATTTGATCAACAATTTAACATTTTGATG AGATTTATTGTGGATCAAACTCAAACTCCAAACCTCAAGGTCAAAGTTGCAATCCTGAAATACATTGAGT CTCTGGCCAGACAGATGGATCCAACAGATTTTGTAAACTCTAGTGAGACAAGGCTTGCTGTTTCTAGAAT CATAACCTGGACAACAGAACCAAAGAGTTCAGACGTGAGAAAGGCAGCACAGATTGTGCTAATCTCTCTG TTTGAATTGAATACTCCTGAATTTACCATGTTACTTGGTGCCTTGCCAAAAACATTCCAGGATGGTGCCA CCAAACTCCTGCACAACCACCTCAAGAATTCCAGTAACACCAGTGTGGGCTCTCCAAGCAATACGATTGG CCGGACGCCCTCCCGACACACCAGCAGCAGGACCAGCCCCCTGACCTCACCCACCAACTGTTCCCATGGG GGTCTGTCTCCAAGTCGGTTATGGGGTTGGAGTGCCGACGGGTTAGCGAAGCACCCACCTCCCTTTTCTC AGCCTAACTCCATCCCCACCGCTCCCTCCCACAAGGCTCTCAGGCGCTCTTACTCTCCCAGCATGCTGGA CTATGATACAGAGAACCTGAACTCTGAAGAAATCTATAGTTCTCTACGTGGAGTTACAGAAGCCATTGAA AAGTTTAGTTTTCGAAGCCAAGAAGATCTGAATGAGCCAATTAAACGAGATGGCAAAAAGGAGTGTGATA TTGTGTCCCGCGATGGGGGCGCTGCCTCCCCTGCCACTGAGGGCCGGGGGGGTAGTGAAGTAGAAGGAGG CCGGACAGCTCTGGATAACAAGACCTCACTACTCAACACCCAGCCTCCGCGCGCCTTCCCGGGGCCGCGG GCGCGAGACTACAACCCGTACCCCTACTCAGATGCCATCAACACCTACGACAAGACCGCCCTGAAAGAGG CTGTGTTCGATGACGACATGGAGCAGCTTCGAGACGTGCCCATCGACCATTCTGACCTGGTGGCTGACCT TCTGAAAGAGCTGTCCAACCACAATGAGCGAGTGGAGGAACGGAAGGGAGCCCTGCTGGAGCTGCTCAAG ATCACGCGGGAAGACAGCCTTGGTGTCTGGGAGGAGCACTTCAAGACCATTCTGCTCCTGCTGCTGGAGA CCCTTGGAGACAAAGACCATTCAATTCGAGCACTGGCGTTAAGAGTTTTGAGGGAAATTCTGAGAAATCA ACCAGCAAGATTTAAAAACTACGCCGAGCTGACGATTATGAAGACTCTGGAAGCCCACAAAGACTCCCAT AAGGAGGTGGTGAGAGCGGCTGAGGAGGCTGCGTCCACACTGGCCAGTTCCATCCACCCGGAGCAGTGCA TCAAGGTGCTCTGCCCCATCATCCAGACGGCCGACTACCCCATCAACCTTGCTGCCATCAAGATGCAGAC CAAAGTCGTCGAGAGGATCGCAAAGGAGTCATTGCTGCAGCTCCTTGTCGACATCATCCCAGGCTTGCTG CAGGGTTATGACAACACCGAAAGTAGTGTGCGTAAGGCCAGCGTGTTTTGCTTAGTGGCAATTTATTCCG TAATCGGAGAAGACCTGAAACCTCACCTTGCACAGCTCACAGGGAGCAAGATGAAGCTACTAAACTTATA CATAAAGAGGGCCCAGACCACCAACAGCAACAGCAGCTCCTCCTCCGATGTCTCCACGCACAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217019 representing NM_015282
Red=Cloning site Green=Tags(s) MEPRMESCLAQVLQKDVGKRLQVGQELIDYFSDKQKSADLEHDQTMLDKLVDGLATSWVNSSNYKVVLLG MDILSALVTRLQDRFKAQIGTVLPSLIDRLGDAKDSVREQDQTLLLKIMDQAANPQYVWDRMLGGFKHKN FRTREGICLCLIATLNASGAQTLTLSKIVPHICNLLGDPNSQVRDAAINSLVEIYRHVGERVRADLSKKG LPQSRLNVIFTKFDEVQKSGNMIQSANDKNFDDEDSVDGNRPSSASSTSSKAPPSSRRNVGMGTTRRLGS STLGSKSSAAKEGAGAVDEEDFIKAFDDVPVVQIYSSRDLEESINKIREILSDDKHDWEQRVNALKKIRS LLLAGAAEYDNFFQHLRLLDGAFKLSAKDLRSQVVREACITLGHLSSVLGNKFDHGAEAIMPTIFNLIPN SAKIMATSGVVAVRLIIRHTHIPRLIPVITSNCTSKSVAVRRRCFEFLDLLLQEWQTHSLERHISVLAET IKKGIHDADSEARIEARKCYWGFHSHFSREAEHLYHTLESSYQKALQSHLKNSDSIVSLPQSDRSSSSSQ ESLNRPLSAKRSPTGSTTSRASTVSTKSVSTTGSLQRSRSDIDVNAAASAKSKVSSSSGTTPFSSAAALP PGSYASLGRIRTRRQSSGSATNVASTPDNRGRSRAKVVSQSQRSRSANPAGAGSRSSSPGKLLGSGYGGL TGGSSRGPPVTPSSEKRSKIPRSQGCSRETSPNRIGLARSSRIPRPSMSQGCSRDTSRESSRDTSPARGF PPLDRFGLGQPGRIPGSVNAMRVLSTSTDLEAAVADALKKPVRRRYEPYGMYSDDDANSDASSVCSERSY GSRNGGIPHYLRQTEDVAEVLNHCASSNWSERKEGLLGLQNLLKSQRTLSRVELKRLCEIFTRMFADPHS KRVFSMFLETLVDFIIIHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDVTRDSFPFDQQFNILM RFIVDQTQTPNLKVKVAILKYIESLARQMDPTDFVNSSETRLAVSRIITWTTEPKSSDVRKAAQIVLISL FELNTPEFTMLLGALPKTFQDGATKLLHNHLKNSSNTSVGSPSNTIGRTPSRHTSSRTSPLTSPTNCSHG GLSPSRLWGWSADGLAKHPPPFSQPNSIPTAPSHKALRRSYSPSMLDYDTENLNSEEIYSSLRGVTEAIE KFSFRSQEDLNEPIKRDGKKECDIVSRDGGAASPATEGRGGSEVEGGRTALDNKTSLLNTQPPRAFPGPR ARDYNPYPYSDAINTYDKTALKEAVFDDDMEQLRDVPIDHSDLVADLLKELSNHNERVEERKGALLELLK ITREDSLGVWEEHFKTILLLLLETLGDKDHSIRALALRVLREILRNQPARFKNYAELTIMKTLEAHKDSH KEVVRAAEEAASTLASSIHPEQCIKVLCPIIQTADYPINLAAIKMQTKVVERIAKESLLQLLVDIIPGLL QGYDNTESSVRKASVFCLVAIYSVIGEDLKPHLAQLTGSKMKLLNLYIKRAQTTNSNSSSSSDVSTHS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015282 |
ORF Size | 4614 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015282.3 |
RefSeq Size | 8089 bp |
RefSeq ORF | 4617 bp |
Locus ID | 23332 |
UniProt ID | Q7Z460 |
Gene Summary | CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217019 | CLASP1 (Myc-DDK-tagged)-Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 1 |
CNY 15,784.00 |
|
RC217019L3 | Lenti-ORF clone of CLASP1 (Myc-DDK-tagged)-Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 1 |
CNY 17,200.00 |
|
RC217019L4 | Lenti-ORF clone of CLASP1 (mGFP-tagged)-Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 1 |
CNY 17,200.00 |
|
SC304281 | CLASP1 (untagged)-Human cytoplasmic linker associated protein 1 (CLASP1), transcript variant 1 |
CNY 29,550.00 |