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PMPCA (NM_015160) Human Tagged ORF Clone
CAT＃: RG217017
- TrueORF®
PMPCA (tGFP-tagged) - Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein
ORF Plasmid: DDK
"NM_015160" in other vectors (6)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Alpha-MPP; CLA1; CPD3; INPP5E; MAS2; P-55; SCAR2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217017 representing NM_015160
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTGTGGTGCTGGCGGCGACGCGGTTGCTGCGGGGCTCGGGTTCTTGGGGCTGTTCGCGGCTGA GGTTTGGACCTCCTGCGTACAGACGGTTTAGTAGTGGTGGTGCCTATCCCAACATCCCCCTCTCTTCTCC CTTACCTGGAGTACCCAAGCCTGTTTTTGCTACAGTTGATGGACAGGAAAAGTTTGAAACCAAAGTAACC ACATTGGATAATGGGCTTCGCGTGGCATCTCAGAATAAGTTTGGACAGTTTTGTACAGTAGGAATTCTTA TCAATTCAGGATCGAGATATGAAGCGAAATACCTTAGTGGAATTGCTCACTTTTTGGAAAAATTGGCATT TTCGTCTACTGCTCGATTTGACAGCAAAGATGAAATTCTGCTTACGTTGGAAAAGCATGGGGGTATCTGT GACTGCCAGACATCAAGAGACACCACCATGTATGCTGTGTCTGCTGATAGCAAAGGCTTGGACACGGTGG TTGCCTTACTGGCTGATGTGGTTCTGCAGCCCCGGCTAACAGATGAAGAAGTCGAGATGACGCGGATGGC GGTCCAGTTTGAGCTGGAGGACCTGAACCTGCGGCCTGACCCAGAGCCACTTCTCACCGAGATGATTCAT GAAGCGGCTTACAGGGAGAACACAGTTGGCCTCCACCGTTTCTGCCCCACAGAAAACGTAGCAAAGATCA ACCGAGAGGTGCTGCATTCCTACCTGAGGAACTACTACACTCCCGACCGCATGGTGCTGGCCGGCGTGGG CGTGGAGCACGAGCATCTGGTGGACTGTGCCCGGAAGTACCTCCTGGGGGTCCAGCCGGCCTGGGGGAGC GCAGAGGCCGTGGATATTGACAGATCTGTGGCCCAGTACACTGGGGGGATTGCCAAGCTAGAAAGAGACA TGTCCAATGTCAGCCTGGGCCCGACCCCCATCCCCGAGCTCACGCACATCATGGTTGGACTGGAGAGCTG CTCCTTCCTGGAGGAGGACTTCATCCCCTTTGCAGTGTTGAACATGATGATGGGCGGAGGTGGCTCCTTC TCGGCTGGTGGGCCCGGCAAGGGCATGTTCTCCAGGCTCTACCTCAACGTGCTCAACAGGCACCACTGGA TGTATAACGCGACCTCCTACCACCACAGCTACGAGGACACTGGCCTCCTTTGCATCCATGCCAGCGCCGA CCCAAGACAGGTTCGAGAAATGGTAGAAATCATCACAAAGGAGTTTATTTTAATGGGCGGAACCGTGGAC ACGGTGGAGCTGGAACGAGCCAAGACGCAGCTGACATCAATGCTCATGATGAACCTGGAATCCAGGCCTG TGATCTTCGAGGATGTGGGGAGGCAGGTGCTGGCCACTCGCTCCAGAAAGCTGCCGCACGAGCTGTGCAC GCTCATCCGCAACGTGAAGCCGGAAGATGTGAAGAGAGTCGCTTCTAAGATGCTCCGAGGGAAGCCGGCA GTGGCCGCCCTGGGTGACCTGACTGACCTGCCCACGTATGAGCACATCCAGACCGCCCTGTCGAGTAAGG ACGGGCGCCTGCCCAGGACGTACCGGCTCTTCCGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217017 representing NM_015160
Red=Cloning site Green=Tags(s) MAAVVLAATRLLRGSGSWGCSRLRFGPPAYRRFSSGGAYPNIPLSSPLPGVPKPVFATVDGQEKFETKVT TLDNGLRVASQNKFGQFCTVGILINSGSRYEAKYLSGIAHFLEKLAFSSTARFDSKDEILLTLEKHGGIC DCQTSRDTTMYAVSADSKGLDTVVALLADVVLQPRLTDEEVEMTRMAVQFELEDLNLRPDPEPLLTEMIH EAAYRENTVGLHRFCPTENVAKINREVLHSYLRNYYTPDRMVLAGVGVEHEHLVDCARKYLLGVQPAWGS AEAVDIDRSVAQYTGGIAKLERDMSNVSLGPTPIPELTHIMVGLESCSFLEEDFIPFAVLNMMMGGGGSF SAGGPGKGMFSRLYLNVLNRHHWMYNATSYHHSYEDTGLLCIHASADPRQVREMVEIITKEFILMGGTVD TVELERAKTQLTSMLMMNLESRPVIFEDVGRQVLATRSRKLPHELCTLIRNVKPEDVKRVASKMLRGKPA VAALGDLTDLPTYEHIQTALSSKDGRLPRTYRLFR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015160 |
| ORF Size | 1575 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015160.3 |
| RefSeq Size | 2097 bp |
| RefSeq ORF | 1578 bp |
| Locus ID | 23203 |
| UniProt ID | Q10713 |
| Domains | Peptidase_M16, Peptidase_M16_C |
| Protein Families | Druggable Genome, Protease |
| Gene Summary | The protein encoded by this gene is found in the mitochondrion, where it represents the alpha subunit of a proteolytic heterodimer. This heterodimer is responsible for cleaving the transit peptide from nuclear-encoded mitochondrial proteins. Defects in this gene are a cause of spinocerebellar ataxia, autosomal recessive 2. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217017 | PMPCA (Myc-DDK-tagged)-Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein |
CNY 3,912.00 |
|
| RC217017L1 | Lenti ORF clone of Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,312.00 |
|
| RC217017L2 | Lenti ORF clone of Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
| RC217017L3 | Lenti ORF clone of Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC217017L4 | Lenti ORF clone of Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
| SC100435 | PMPCA (untagged)-Human peptidase (mitochondrial processing) alpha (PMPCA), nuclear gene encoding mitochondrial protein |
CNY 3,920.00 |
