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SIN3B (NM_015260) Human Tagged ORF Clone
CAT＃: RG216859
- TrueORF®
SIN3B (tGFP-tagged) - Human SIN3 homolog B, transcription regulator (yeast) (SIN3B)
ORF Plasmid: DDK
"NM_015260" in other vectors (6)
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CNY 12,730.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216859 representing NM_015260
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCACGCTGGCGGTGGCAGCGGTGGCAGCGGTGCCGGCGGCCCCGCGGGCCGGGGGCTGAGCGGCG CCCGCTGGGGTCGCTCGGGCTCCGCAGGCCACGAGAAGCTGCCGGTGCACGTAGAAGACGCCCTCACCTA TCTGGACCAGGTGAAGATCCGCTTTGGCAGCGACCCTGCCACCTACAACGGCTTCCTGGAGATCATGAAG GAGTTCAAAAGCCAGAGCATCGATACTCCTGGAGTCATCAGACGTGTCTCGCAGCTCTTCCACGAGCACC CTGACCTCATTGTTGGATTCAACGCTTTTCTTCCCCTCGGATATAGAATAGACATTCCCAAGAATGGCAA GTTAAACATACAGTCGCCTCTGACAAGCCAGGAGAATTCGCACAACCACGGGGACGGTGCAGAGGACTTC AAGCAGCAGGTGCCGTATAAAGAGGACAAACCCCAGGTGCCCCTGGAGTCCGATTCCGTGGAATTCAACA ACGCCATCAGCTATGTGAATAAGATTAAAACCCGCTTCCTAGACCACCCAGAAATCTACAGGTCATTCCT GGAGATCCTGCACACGTACCAGAAGGAGCAGCTGAACACGAGGGGCCGGCCATTCCGAGGCATGTCTGAA GAGGAGGTGTTCACCGAGGTGGCCAACCTCTTCCGGGGCCAGGAGGACCTGCTCTCAGAGTTTGGACAGT TCCTGCCCGAAGCCAAGCGGTCTCTGTTCACAGGAAACGGGCCGTGCGAGATGCACAGCGTGCAGAAGAA CGAGCACGACAAGACCCCGGAGCACAGCAGGAAGCGCTCCCGGCCCTCGCTCCTCCGCCCCGTGTCTGCA CCCGCCAAGAAAAAAATGAAACTTCGTGGTACCAAAGACCTGTCCATCGCTGCAGTGGGGAAGTACGGGA CTCTGCAGGAATTTTCTTTCTTTGACAAGGTCCGCCGGGTGCTGAAGAGCCAGGAGGTGTATGAAAACTT CCTCCGCTGCATCGCACTCTTCAACCAGGAGCTGGTGTCTGGCTCTGAGCTCCTGCAGCTCGTCAGCCCA TTTCTGGGGAAATTTCCAGAACTCTTTGCACAGTTCAAGTCCTTCCTGGGGGTAAAAGAGCTGTCCTTCG CGCCACCCATGAGCGACAGATCCGGGGACGGGATAAGCCGGGAAATTGATTATGCATCCTGCAAGCGCAT AGGATCCAGCTACCGGGCACTCCCCAAAACCTACCAGCAGCCCAAGTGCAGTGGGAGGACAGCCATCTGC AAGGAGCTTGACCATTGGACACTTCTCCAGGGTTCGTGGACAGACGATTACTGCATGTCCAAGTTCAAGA ATACCTGCTGGATTCCAGGATATAGTGCAGGGGTACTGAACGACACCTGGGTCTCCTTCCCTTCCTGGTC TGAGGACTCCACGTTCGTCAGCTCCAAGAAGACACCGTACGAGGAGCAGCTTCACCGCTGTGAGGACGAG CGCTTCGAGTTAGACGTTGTCCTGGAGACGAACCTGGCCACAATCCGTGTGTTGGAAAGTGTGCAGAAGA AGCTGTCTCGGATGGCGCCGGAAGACCAGGAGAAGTTCCGGCTGGACGACTCCCTGGGAGGCACGTCGGA GGTGATCCAGCGCCGTGCCATTTATCGCATCTATGGCGACAAGGCCCCGGAGATCATCGAGAGCCTCAAG AAGAACCCTGTCACCGCTGTCCCCGTTGTCCTGAAAAGACTGAAGGCCAAGGAAGAGGAGTGGCGGGAGG CCCAGCAGGGCTTCAACAAGATCTGGCGGGAGCAGTATGAGAAGGCGTACCTCAAGTCCCTTGACCACCA GGCTGTGAACTTCAAGCAGAACGACACCAAGGCCCTGCGCTCCAAGAGCTTGCTCAACGAGATCGAGAGC GTCTACGACGAGCACCAGGAGCAGCACTCGGAGGGCCGCAGTGCCCCCTCTAGCGAGCCGCACCTCATCT TTGTGTACGAGGACCGGCAGATCCTGGAGGACGCAGCAGCGCTCATCAGCTACTACGTGAAGCGGCAGCC GGCCATCCAGAAGGAGGACCAGGGCACCATCCACCAGCTGCTGCACCAGTTCGTGCCCAGCCTCTTCTTC TCTCAGCAGCTGGACCTGGGCGCCTCCGAGGAGTCAGCTGATGAGGACCGGGACAGCCCCCAGGGGCAGA CCACAGACCCCAGTGAGCGGAAGAAGCCGGCGCCAGGACCCCACAGTAGCCCCCCAGAGGAGAAGGGGGC CTTCGGGGATGCCCCGGCCACTGAGCAGCCACCCCTGCCGCCCCCAGCCCCGCACAAGCCCCTGGACGAT GTCTACAGCCTATTTTTTGCCAACAACAACTGGTACTTCTTCCTGCGCCTGCACCAGACCCTGTGCTCCA GGCTGCTGAAGATCTACCGCCAGGCGCAGAAGCAGCTTCTGGAGTATCGGACCGAGAAGGAGCGGGAGAA GCTGCTGTGTGAGGGCCGCAGGGAGAAGGGCAGCGACCCCGCCATGGAGCTGCGGCTGAAGCAGCCCAGT GAAGTGGAGCTGGAGGAGTACTACCCGGCCTTCCTGGACATGGTGCGGAGCCTGCTGGAGGGCAGCATCG ACCCCACGCAGTACGAGGACACCCTACGCGAGATGTTCACCATCCATGCCTACGTGGGCTTCACCATGGA CAAGCTGGTGCAGAACATTGCGCGGCAGCTGCACCACCTCGTGAGCGATGACGTCTGCCTGAAGGTGGTG GAGCTCTACCTGAACGAGAAGAAGCGGGGTGCCGCTGGTGGGAACCTGTCCTCCCGCTGCGTCCGCGCTG CTAGGGAGACCAGCTACCAGTGGAAGGCTGAGCGCTGCATGGCCGACGAGAACTGCTTCAAGGTGATGTT CCTGCAGCGCAAAGGGCAGGTGATCATGACCATCGAGCTCCTGGACACCGAGGAGGCCCAGACGGAGGAC CCTGTGGAGGTCCAGCACCTGGCTCGGTACGTGGAGCAGTATGTGGGGACCGAGGGCGCGTCCAGCTCGC CCACTGAGGGCTTCCTCCTGAAACCTGTGTTCCTGCAGAGGAACCTCAAGAAGTTCCGCCGCCGGTGGCA GAGCGAGCAGGCGCGGGCCCTGCGCGGTGAGGCCAGGAGCTCCTGGAAGCGGCTGGTGGGCGTGGAGAGC GCCTGCGACGTGGACTGCCGCTTCAAGCTCAGCACTCACAAGATGGTGTTCATCGTGAACTCCGAGGACT ACATGTACCGTCGCGGGACCCTCTGCCGGGCCAAGCAGGTGCAGCCCCTGGTCCTGCTCCGCCACCACCA GCACTTTGAGGAGTGGCACAGCCGCTGGCTGGAGGACAATGTGACGGTGGAGGCGGCTAGCCTGGTGCAG GACTGGCTGATGGGTGAGGAGGACGAGGACATGGTACCCTGCAAGACGCTGTGTGAGACAGTGCACGTGC ACGGCCTGCCCGTGACCCGCTACCGCGTGCAGTACAGCCGCCGCCCGGCCTCGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216859 representing NM_015260
Red=Cloning site Green=Tags(s) MAHAGGGSGGSGAGGPAGRGLSGARWGRSGSAGHEKLPVHVEDALTYLDQVKIRFGSDPATYNGFLEIMK EFKSQSIDTPGVIRRVSQLFHEHPDLIVGFNAFLPLGYRIDIPKNGKLNIQSPLTSQENSHNHGDGAEDF KQQVPYKEDKPQVPLESDSVEFNNAISYVNKIKTRFLDHPEIYRSFLEILHTYQKEQLNTRGRPFRGMSE EEVFTEVANLFRGQEDLLSEFGQFLPEAKRSLFTGNGPCEMHSVQKNEHDKTPEHSRKRSRPSLLRPVSA PAKKKMKLRGTKDLSIAAVGKYGTLQEFSFFDKVRRVLKSQEVYENFLRCIALFNQELVSGSELLQLVSP FLGKFPELFAQFKSFLGVKELSFAPPMSDRSGDGISREIDYASCKRIGSSYRALPKTYQQPKCSGRTAIC KELDHWTLLQGSWTDDYCMSKFKNTCWIPGYSAGVLNDTWVSFPSWSEDSTFVSSKKTPYEEQLHRCEDE RFELDVVLETNLATIRVLESVQKKLSRMAPEDQEKFRLDDSLGGTSEVIQRRAIYRIYGDKAPEIIESLK KNPVTAVPVVLKRLKAKEEEWREAQQGFNKIWREQYEKAYLKSLDHQAVNFKQNDTKALRSKSLLNEIES VYDEHQEQHSEGRSAPSSEPHLIFVYEDRQILEDAAALISYYVKRQPAIQKEDQGTIHQLLHQFVPSLFF SQQLDLGASEESADEDRDSPQGQTTDPSERKKPAPGPHSSPPEEKGAFGDAPATEQPPLPPPAPHKPLDD VYSLFFANNNWYFFLRLHQTLCSRLLKIYRQAQKQLLEYRTEKEREKLLCEGRREKGSDPAMELRLKQPS EVELEEYYPAFLDMVRSLLEGSIDPTQYEDTLREMFTIHAYVGFTMDKLVQNIARQLHHLVSDDVCLKVV ELYLNEKKRGAAGGNLSSRCVRAARETSYQWKAERCMADENCFKVMFLQRKGQVIMTIELLDTEEAQTED PVEVQHLARYVEQYVGTEGASSSPTEGFLLKPVFLQRNLKKFRRRWQSEQARALRGEARSSWKRLVGVES ACDVDCRFKLSTHKMVFIVNSEDYMYRRGTLCRAKQVQPLVLLRHHQHFEEWHSRWLEDNVTVEAASLVQ DWLMGEEDEDMVPCKTLCETVHVHGLPVTRYRVQYSRRPASP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015260 |
| ORF Size | 3486 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015260.4 |
| RefSeq Size | 5129 bp |
| RefSeq ORF | 3489 bp |
| Locus ID | 23309 |
| UniProt ID | O75182 |
| Gene Summary | Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216859 | SIN3B (Myc-DDK-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B) |
CNY 12,136.00 |
|
| RC216859L1 | Lenti-ORF clone of SIN3B (Myc-DDK-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B) |
CNY 14,536.00 |
|
| RC216859L2 | Lenti-ORF clone of SIN3B (mGFP-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B) |
CNY 13,490.00 |
|
| RC216859L3 | Lenti-ORF clone of SIN3B (Myc-DDK-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B) |
CNY 13,490.00 |
|
| RC216859L4 | Lenti-ORF clone of SIN3B (mGFP-tagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B) |
CNY 13,490.00 |
|
| SC304274 | SIN3B (untagged)-Human SIN3 homolog B, transcription regulator (yeast) (SIN3B) |
CNY 22,330.00 |
