HCN4 (NM_005477) Human Tagged ORF Clone
CAT#: RG216770
- TrueORF®
HCN4 (tGFP-tagged) - Human hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4)
ORF Plasmid: DDK
"NM_005477" in other vectors (4)
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CNY 9,976.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SSS2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG216770 representing NM_005477
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAAGCTGCCGCCGTCCATGCGCAAGCGGCTCTACAGCCTCCCGCAGCAGGTGGGGGCCAAGGCGT GGATCATGGACGAGGAAGAGGACGCCGAGGAGGAGGGGGCCGGGGGCCGCCAAGACCCCAGCCGCAGGAG CATCCGGCTGCGGCCACTGCCCTCGCCCTCCCCCTCGGCGGCCGCGGGTGGCACGGAGTCCCGGAGCTCG GCCCTCGGGGCAGCGGACAGCGAAGGGCCGGCCCGCGGCGCGGGCAAGTCCAGCACGAACGGCGACTGCA GGCGCTTCCGCGGGAGCCTGGCCTCGCTGGGCAGCCGGGGCGGCGGCAGCGGCGGCACGGGGAGCGGCAG CAGTCACGGACACCTGCATGACTCCGCGGAGGAGCGGCGGCTCATCGCCGAGGGCGACGCGTCCCCCGGC GAGGACAGGACGCCCCCAGGCCTGGCGGCCGAGCCCGAGCGCCCCGGCGCCTCGGCGCAGCCCGCAGCCT CGCCGCCGCCGCCCCAGCAGCCACCGCAGCCGGCCTCCGCCTCCTGCGAGCAGCCCTCGGTGGACACCGC TATCAAAGTGGAGGGAGGCGCGGCTGCCGGCGACCAGATCCTCCCGGAGGCCGAGGTGCGCCTGGGCCAG GCCGGCTTCATGCAGCGCCAGTTCGGGGCCATGCTCCAACCCGGGGTCAACAAATTCTCCCTAAGGATGT TCGGCAGCCAGAAAGCCGTGGAGCGCGAACAGGAGAGGGTCAAGTCGGCCGGATTTTGGATTATCCACCC CTACAGTGACTTCAGATTTTACTGGGACCTGACCATGCTGCTGCTGATGGTGGGAAACCTGATTATCATT CCTGTGGGCATCACCTTCTTCAAGGATGAGAACACCACACCCTGGATTGTCTTCAATGTGGTGTCAGACA CATTCTTCCTCATCGACTTGGTCCTCAACTTCCGCACAGGGATCGTGGTGGAGGACAACACAGAGATCAT CCTGGACCCGCAGCGGATTAAAATGAAGTACCTGAAAAGCTGGTTCATGGTAGATTTCATTTCCTCCATC CCCGTGGACTACATCTTCCTCATTGTGGAGACACGCATCGACTCGGAGGTCTACAAGACTGCCCGGGCCC TGCGCATTGTCCGCTTCACGAAGATCCTCAGCCTCTTACGCCTGTTACGCCTCTCCCGCCTCATTCGATA TATTCACCAGTGGGAAGAGATCTTCCACATGACCTACGACCTGGCCAGCGCCGTGGTGCGCATCGTGAAC CTCATCGGCATGATGCTCCTGCTCTGCCACTGGGACGGCTGCCTGCAGTTCCTGGTACCCATGCTACAGG ACTTCCCTGACGACTGCTGGGTGTCCATCAACAACATGGTGAACAACTCCTGGGGGAAGCAGTACTCCTA CGCGCTCTTCAAGGCCATGAGCCACATGCTGTGCATCGGCTACGGGCGGCAGGCGCCCGTGGGCATGTCC GACGTCTGGCTCACCATGCTCAGCATGATCGTGGGTGCCACCTGCTACGCCATGTTCATTGGCCACGCCA CTGCCCTCATCCAGTCCCTGGACTCCTCCCGGCGCCAGTACCAGGAAAAGTACAAGCAGGTGGAGCAGTA CATGTCCTTTCACAAGCTCCCGCCCGACACCCGGCAGCGCATCCACGACTACTACGAGCACCGCTACCAG GGCAAGATGTTCGACGAGGAGAGCATCCTGGGCGAGCTAAGCGAGCCCCTGCGGGAGGAGATCATCAACT TTAACTGTCGGAAGCTGGTGGCCTCCATGCCACTGTTTGCCAATGCGGACCCCAACTTCGTGACGTCCAT GCTGACCAAGCTGCGTTTCGAGGTCTTCCAGCCTGGGGACTACATCATCCGGGAAGGCACCATTGGCAAG AAGATGTACTTCATCCAGCATGGCGTGGTCAGCGTGCTCACCAAGGGCAACAAGGAGACCAAGCTGGCCG ACGGCTCCTACTTTGGAGAGATCTGCCTGCTGACCCGGGGCCGGCGCACAGCCAGCGTGAGGGCCGACAC CTACTGCCGCCTCTACTCGCTGAGCGTGGACAACTTCAATGAGGTGCTGGAGGAGTACCCCATGATGCGA AGGGCCTTCGAGACCGTGGCGCTGGACCGCCTGGACCGCATTGGCAAGAAGAACTCCATCCTCCTCCACA AAGTCCAGCACGACCTCAACTCCGGCGTCTTCAACTACCAGGAGAATGAGATCATCCAGCAGATTGTGCA GCATGACCGGGAGATGGCCCACTGCGCGCACCGCGTCCAGGCTGCTGCCTCTGCCACCCCAACCCCCACG CCCGTCATCTGGACCCCGCTGATCCAGGCACCACTGCAGGCTGCCGCTGCCACCACTTCTGTGGCCATAG CCCTCACCCACCACCCTCGCCTGCCTGCTGCCATCTTCCGCCCTCCCCCAGGATCTGGGCTGGGCAACCT CGGTGCCGGGCAGACGCCAAGGCACCTGAAACGGCTGCAGTCCCTGATCCCTTCTGCGCTGGGCTCCGCC TCGCCCGCCAGCAGCCCGTCCCAGGTGGACACACCGTCTTCATCCTCCTTCCACATCCAACAGCTGGCTG GATTCTCTGCCCCCGCTGGACTGAGCCCACTCCTGCCCTCATCCAGCTCCTCCCCACCCCCCGGGGCCTG TGGCTCCCCCTCGGCTCCCACACCATCAGCTGGCGTAGCCGCCACCACCATAGCCGGGTTTGGCCACTTC CACAAGGCGCTGGGTGGCTCCCTGTCCTCCTCCGACTCTCCCCTGCTCACCCCGCTGCAGCCAGGCGCCC GCTCCCCGCAGGCTGCCCAGCCATCTCCCGCGCCACCCGGGGCCCGGGGAGGCCTGGGACTCCCGGAGCA CTTCCTGCCACCCCCACCCTCATCCAGATCCCCGTCATCTAGCCCCGGGCAGCTGGGCCAGCCTCCCGGG GAGTTGTCCCTAGGTCTGGCCACTGGCCCACTGAGCACGCCAGAGACACCCCCACGGCAGCCTGAGCCGC CGTCCCTTGTGGCAGGGGCCTCTGGGGGGGCTTCCCCTGTAGGCTTTACTCCCCGAGGAGGTCTCAGCCC CCCTGGCCACAGCCCAGGCCCCCCAAGAACCTTCCCGAGTGCCCCGCCCCGGGCCTCTGGCTCCCACGGA TCCTTGCTCCTGCCACCTGCATCCAGCCCCCCACCACCCCAGGTCCCCCAGCGCCGGGGCACACCCCCGC TCACCCCCGGCCGCCTCACCCAGGACCTCAAGCTCATCTCCGCGTCTCAGCCAGCCCTGCCTCAGGACGG GGCGCAGACTCTCCGCAGAGCCTCCCCGCACTCCTCAGGGGAGTCCATGGCTGCCTTCCCGCTCTTCCCC AGGGCTGGGGGTGGCAGCGGGGGCAGTGGGAGCAGCGGGGGCCTCGGTCCCCCTGGGAGGCCCTATGGTG CCATCCCCGGCCAGCACGTCACTCTGCCTCGGAAGACATCCTCAGGTTCTTTGCCACCCCCTCTGTCTTT GTTTGGGGCAAGAGCCACCTCTTCTGGGGGGCCCCCTCTGACTGCTGGACCCCAGAGGGAACCTGGGGCC AGGCCTGAGCCAGTGCGCTCCAAACTGCCATCCAATCTA CTCGAG - GFP Tag - GTTTAA >RG216770 representing NM_005477
Red=Cloning site Green=Tags(s) MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTESRSS ALGAADSEGPARGAGKSSTNGDCRRFRGSLASLGSRGGGSGGTGSGSSHGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERPGASAQPAASPPPPQQPPQPASASCEQPSVDTAIKVEGGAAAGDQILPEAEVRLGQ AGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIII PVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFMVDFISSI PVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVN LIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMS DVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQ GKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGK KMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMR RAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPT PVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGSGLGNLGAGQTPRHLKRLQSLIPSALGSA SPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGACGSPSAPTPSAGVAATTIAGFGHF HKALGGSLSSSDSPLLTPLQPGARSPQAAQPSPAPPGARGGLGLPEHFLPPPPSSRSPSSSPGQLGQPPG ELSLGLATGPLSTPETPPRQPEPPSLVAGASGGASPVGFTPRGGLSPPGHSPGPPRTFPSAPPRASGSHG SLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESMAAFPLFP RAGGGSGGSGSSGGLGPPGRPYGAIPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAGPQREPGA RPEPVRSKLPSNL LE - GFP Tag - V |
Restriction Sites |
SgfI-XhoI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_005477 |
ORF Size | 3609 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005477.3 |
RefSeq Size | 5065 bp |
RefSeq ORF | 3612 bp |
Locus ID | 10021 |
UniProt ID | Q9Y3Q4 |
Domains | cNMP, ion_trans |
Protein Families | Druggable Genome, Ion Channels: Cyclic nucleotide gated, Transmembrane |
Gene Summary | This gene encodes a member of the hyperpolarization-activated cyclic nucleotide-gated potassium channels. The encoded protein shows slow kinetics of activation and inactivation, and is necessary for the cardiac pacemaking process. This channel may also mediate responses to sour stimuli. Mutations in this gene have been linked to sick sinus syndrome 2, also known as atrial fibrillation with bradyarrhythmia or familial sinus bradycardia. Two pseudogenes have been identified on chromosome 15. [provided by RefSeq, Oct 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216770 | HCN4 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) |
CNY 8,376.00 |
|
RC216770L3 | Lenti-ORF clone of HCN4 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) |
CNY 13,870.00 |
|
RC216770L4 | Lenti-ORF clone of HCN4 (mGFP-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) |
CNY 13,870.00 |
|
SC303651 | HCN4 (untagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4) |
CNY 8,384.00 |