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GPRASP1 (NM_014710) Human Tagged ORF Clone
CAT＃: RG216371
- TrueORF®
GPRASP1 (tGFP-tagged) - Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1
ORF Plasmid: DDK
"NM_014710" in other vectors (6)
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CNY 15,300.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GASP; GASP-1; GASP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216371 representing NM_014710
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGGGGCAGAGATTGAGTCTGGTGCCCAGGTCAAGCCTGAAAAGAAGCCTGGGGAAGAGGTTGTAG GTGGGGCTGAGATAGAGAATGATGTCCCTCTGGTGGTCAGACCCAAGGTTAGGACCCAGGCCCAGATAAT GCCTGGGGCAAGGCCCAAGAATAAGTCCAAGGTTATGCCTGGAGCAAGCACCAAAGTTGAGACAAGTGCA GTGGGTGGGGCACGCCCTAAGAGTAAGGCCAAGGCAATACCTGTTTCACGATTTAAGGAAGAAGCCCAGA TGTGGGCTCAGCCCAGGTTTGGTGCTGAAAGATTGTCTAAGACAGAGAGAAACTCCCAGACCAATATCAT AGCCTCTCCACTTGTCAGTACTGATTCTGTCTTGGTTGCTAAAACAAAGTACCTGTCTGAGGATAGAGAA CTGGTTAATACAGACACTGAGAGCTTTCCTAGAAGGAAGGCCCATTACCAAGCAGGATTCCAGCCTTCTT TTAGGTCAAAGGAGGAGACCAATATGGGGTCCTGGTGCTGTCCTAGGCCTACATCCAAACAAGAAGCCTC TCCTAATTCTGATTTCAAATGGGTAGACAAATCTGTGAGTTCCTTGTTCTGGAGTGGAGATGAGGTCACT GCAAAATTTCATCCTGGGAATAGGGTAAAAGACAGTAACAGATCCATGCACATGGCCAATCAAGAGGCTA ATACCATGTCTAGGTCCCAAACTAACCAGGAGCTCTATATTGCATCTAGTTCTGGTTCTGAGGATGAGTC TGTTAAGACACCCTGGTTCTGGGCCAGAGATAAAACCAATACCTGGTCTGGGCCCAGGGAAGATCCCAAT AGCAGGTCCAGGTTTAGGTCTAAGAAAGAAGTCTATGTTGAATCAAGTTCTGGATCTGAGCATGAAGACC ATTTGGAGTCCTGGTTTGGGGCTGGAAAGGAGGGCAAATTCAGGTCCAAAATGAGAGCTGGGAAGGAGGC CAATAACAGGGCCAGGCACAGGGCCAAGCGAGAAGCTTGCATTGATTTCATGCCTGGGTCTATAGATGTA ATTAAAAAAGAGTCCTGTTTCTGGCCTGAAGAAAATGCTAATACCTTTTCAAGGCCCATGATCAAGAAAG AGGCCAGGGCCAGAGCAATGACAAAGGAAGAGGCCAAAACCAAGGCCCGAGCCAGGGCCAAGCAAGAAGC CAGGTCAGAGGAGGAAGCCCTCATTGGGACCTGGTTCTGGGCTACAGACGAGTCCAGCATGGCAGATGAA GCCAGCATAGAGTCCAGTCTACAAGTGGAGGATGAGTCCATAATTGGGAGTTGGTTCTGGACTGAAGAAG AGGCCAGTATGGGGACTGGGGCTAGCAGTAAATCCAGACCAAGGACTGATGGGGAGCGTATTGGTGATTC CTTATTTGGGGCTAGGGAAAAGACCAGTATGAAAACTGGGGCTGAGGCCACCTCTGAATCTATACTAGCA GCTGATGATGAACAGGTCATTATTGGTTCCTGGTTCTGGGCTGGTGAAGAGGTCAACCAAGAGGCTGAGG AAGAGACCATTTTTGGGTCGTGGTTCTGGGTCATTGATGCGGCCAGTGTGGAATCTGGTGTTGGGGTCAG CTGTGAGTCCAGGACAAGGTCTGAGGAAGAAGAGGTCATTGGTCCCTGGTTTTGGTCTGGAGAACAAGTT GATATAGAGGCTGGAATCGGAGAAGAGGCCAGGCCAGGAGCTGAAGAAGAGACAATATTCGGGTCCTGGT TTTGGGCTGAAAACCAGACCTATATGGATTGTAGGGCTGAAACTAGCTGTGACACCATGCAAGGGGCTGA GGAGGAGGAGCCCATTATTGGGTCCTGGTTTTGGACCAGAGTAGAAGCTTGTGTGGAGGGTGATGTCAAC AGCAAGTCTAGCCTGGAGGACAAGGAAGAGGCCATGATACCATGTTTTGGAGCCAAAGAAGAGGTCAGTA TGAAGCATGGGACTGGTGTCAGATGCAGATTTATGGCAGGGGCTGAGGAGACCAATAATAAGTCTTGCTT CTGGGCAGAAAAAGAACCCTGTATGTATCCTGCCGGTGGAGGAAGTTGGAAGTCTAGGCCAGAGGAGGAA GAGGACATTGTCAATTCGTGGTTCTGGTCCAGAAAATACACAAAGCCAGAGGCCATTATAGGGTCCTGGT TATGGGCTACAGAAGAGAGTAATATAGATGGGACTGGAGAAAAGGCCAAGTTACTGACTGAAGAGGAGAC CATAATCAATTCCTGGTTCTGGAAAGAAGATGAAGCCATTTCAGAGGCTACTGACAGAGAAGAGTCCAGG CCAGAAGCTGAGGAGGGGGACATTGTTGGTTCTTGGTTCTGGGCTGGAGAAGAGGACAGACTAGAGCCAG CTGCTGAGACTAGAGAAGAAGACAGGCTAGCAGCTGAGAAAGAAGGTATTGTTGGGTCCTGGTTTGGGGC CAGAGAAGAGACCATTAGAAGAGAGGCTGGGTCTTGCAGCAAATCCAGTCCTAAAGCTGAAGAGGAAGAA GTCATTATTGGGTCCTGGTTCTGGGAAGAAGAGGCCAGTCCGGAGGCAGTGGCAGGAGTCGGCTTTGAGT CAAAGCCTGGGACTGAGGAGGAAGAAATCACTGTTGGGTCCTGGTTCTGGCCTGAAGAAGAAGCCAGTAT ACAGGCTGGATCTCAGGCAGTAGAGGAAATGGAGTCAGAGACTGAAGAGGAAACCATTTTTGGGTCCTGG TTCTGGGATGGAAAAGAAGTCAGTGAAGAAGCAGGACCATGCTGTGTATCCAAGCCAGAGGATGATGAAG AGATGATTGTTGAGTCCTGGTTCTGGTCTAGAGACAAAGCCATTAAGGAAACTGGAACTGTGGCCACCTG TGAGTCCAAGCCAGAAAATGAGGAAGGGGCCATTGTTGGGTCTTGGTTTGAGGCTGAAGATGAGGTAGAT AACAGGACTGACAATGGAAGCAACTGTGGGTCCAGGACATTAGCTGATGAAGATGAGGCCATAGTGGGGT CCTGGTTCTGGGCAGGAGATGAGGCCCATTTTGAATCAAATCCTAGCCCCGTGTTCAGGGCCATTTGCAG GTCCACGTGTTCAGTTGAACAGGAGCCTGATCCTTCACGCAGGCCTCAGAGTTGGGAGGAGGTCACTGTT CAGTTCAAGCCTGGTCCATGGGGTAGGGTCGGCTTCCCATCTATAAGCCCCTTTAGATTTCCGAAAGAGG CAGCATCTTTATTCTGTGAAATGTTTGGGGGCAAACCCAGGAACATGGTACTTAGCCCAGAAGGGGAAGA TCAGGAATCTTTGCTTCAGCCTGATCAGCCTAGTCCTGAGTTCCCATTTCAGTATGATCCTTCCTACAGG TCAGTCCAGGAAATTCGAGAGCATCTTAGGGCCAAGGAGAGTACAGAGCCTGAGAGTTCATCCTGTAACT GCATACAATGTGAGCTGAAAATTGGTTCTGAAGAGTTTGAAGAACTCCTTTTATTAATGGAAAAAATTCG GGATCCTTTTATTCATGAAATATCTAAAATCGCAATGGGTATGAGAAGTGCTTCTCAATTTACCCGAGAT TTCATTCGAGATTCAGGTGTTGTCTCACTTATTGAAACCTTGCTTAATTATCCGTCCTCCCGAGTTAGAA CAAGTTTTTTGGAAAATATGATTCGCATGGCCCCACCTTATCCGAATCTAAACATAATTCAGACATACAT ATGTAAAGTGTGTGAGGAAACCCTTGCTTATAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATG ATTAGACATCTCACTACTACTACTGACTATCACACACTGGTTGCCAATTATATGTCTGGGTTTCTCTCCT TATTAGCTACAGGCAATGCCAAAACAAGGTTTCATGTTTTGAAAATGCTACTGAATTTGTCTGAAAATCT TTTCATGACAAAAGAACTACTCAGTGCTGAAGCAGTGTCAGAATTTATAGGCCTCTTTAACAGGGAAGAG ACAAATGACAATATTCAAATTGTTCTTGCAATATTTGAGAATATTGGCAACAATATCAAAAAAGAAACAG TGTTCTCTGATGATGATTTCAATATTGAGCCGCTTATTTCTGCATTCCACAAAGTTGAGAAATTTGCTAA GGAACTGCAAGGCAAAACAGACAATCAAAATGACCCTGAAGGGGACCAAGAAAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216371 representing NM_014710
Red=Cloning site Green=Tags(s) MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSA VGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRE LVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVT AKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPN SRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEGKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDV IKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADE ASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILA ADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQV DIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVN SKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEE EDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESR PEAEEGDIVGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEE VIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSW FWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVD NRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTV QFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYR SVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRD FIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRM IRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREE TNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014710 |
| ORF Size | 4185 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014710.1, NP_055525.1 |
| RefSeq Size | 5190 bp |
| RefSeq ORF | 4188 bp |
| Locus ID | 9737 |
| UniProt ID | Q5JY77 |
| Domains | DUF634 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the GPRASP (G protein-coupled receptor associated sorting protein) family. The protein may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It targets receptors for degradation in lysosomes. The receptors interacting with this sorting protein include D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CB1R). Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, May 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216371 | GPRASP1 (Myc-DDK-tagged)-Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1 |
CNY 9,544.00 |
|
| RC216371L1 | Lenti ORF clone of Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, Myc-DDK-tagged |
CNY 11,944.00 |
|
| RC216371L2 | Lenti ORF clone of Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mGFP tagged |
CNY 15,770.00 |
|
| RC216371L3 | Lenti ORF clone of Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, Myc-DDK-tagged |
CNY 15,770.00 |
|
| RC216371L4 | Lenti ORF clone of Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mGFP tagged |
CNY 15,770.00 |
|
| SC114919 | GPRASP1 (untagged)-Human G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1 |
CNY 9,552.00 |
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