MED15 (NM_001003891) Human Tagged ORF Clone
CAT#: RG215754
- TrueORF®
MED15 (tGFP-tagged) - Human mediator complex subunit 15 (MED15), transcript variant 1
ORF Plasmid: DDK
"NM_001003891" in other vectors (2)
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CNY 6,370.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1; TNRC7 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG215754 representing NM_001003891
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTTTCCGGGCAAGAGACCGACTGGCGGAGCACCGCCTTCCGGCAGAAGCTGGTCAGTCAAATCG AGGATGCCATGAGGAAAGCTGGTGTGGCACACAGTAAATCCAGCAAGGATATGGAGAGCCATGTTTTCCT GAAGGCCAAGACCCGGGACGAATACCTTTCTCTCGTGGCCAGGCTCATTATCCATTTTCGAGACATTCAT AACAAGAAATCTCAAGCTTCCGTCAGTGATCCTATGAATGCACTCCAGAGCCTGACTGGCGGACCTGCTG CGGGAGCCGCTGGAATTGGCATGCCTCCTCGGGGCCCGGGACAGTCTCTGGGCGGGATGGGTAGCCTTGG TGCCATGGGACAGCCAATGTCTCTCTCAGGGCAGCCGCCTCCTGGGACCTCGGGGATGGCCCCTCACAGC ATGGCTGTCGTGTCTACGGCAACTCCACAGACCCAGCTGCAGCTCCAGCAGGTGGCGCTGCAGCAGCAGC AGCAACAGCAGCAGTTCCAGCAGCAGCAGCAGGCGGCGCTACAGCAGCAGCAGCAGCAGCAGCAACAGCA GCAGTTCCAGGCTCAGCAGAGTGCCATGCAGCAGCAGTTCCAAGCAGTAGTGCAGCAGCAGCAGCAGCTC CAGCAGCAGCAGCAGCAGCAGCAGCATCTAATTAAATTGCATCATCAAAATCAGCAACAGATACAGCAGC AGCAACAGCAGCTGCAGCGAATAGCACAGCTGCAGCTCCAACAACAGCAACAGCAGCAGCAGCAGCAGCA GCAGCAGCAGCAGCAGGCTTTGCAGGCCCAGCCACCAATTCAGCAGCCACCGATGCAGCAGCCACAGCCT CCGCCCTCCCAGGCTCTGCCCCAGCAGCTGCAGCAGATGCATCACACACAGCACCACCAGCCGCCACCAC AGCCCCAGCAGCCTCCAGTTGCTCAGAACCAACCATCACAACTCCCGCCACAGTCGCAGACCCAGCCTTT GGTGTCACAGGCGCAAGCTCTCCCTGGACAAATGTTGTATACCCAACCACCACTGAAATTTGTCCGAGCT CCGATGGTGGTGCAGCAGCCCCCAGTGCAGCCCCAGGTGCAGCAGCAGCAGACAGCAGTACAGACAGCTC AGGCTGCCCAGATGGTGGCTCCCGGAGTCCAGATGATCACGGAAGCCTTGGCCCAAGGTGGGATGCACAT AAGAGCCCGGTTCCCGCCTACCACCGCTGTGTCCGCCATCCCGTCAAGCTCCATCCCTTTGGGCAGACAG CCCATGGCACAGGTCAGCCAGAGCAGCCTCCCCATGCTGTCCTCGCCGTCACCGGGCCAGCAGGTGCAGA CCCCGCAGTCGATGCCCCCTCCCCCCCAGCCGTCCCCGCAGCCCGGCCAGCCCAGCTCACAGCCCAACTC CAACGTCAGCTCTGGCCCTGCCCCATCTCCCAGTAGCTTCCTGCCCAGCCCCTCACCGCAGCCCTCCCAG AGCCCAGTGACGGCGCGGACCCCACAGAACTTCAGTGTCCCCTCACCTGGACCTTTAAACACACCTGTGA ACCCCAGCTCTGTCATGAGCCCAGCTGGCTCCAGCCAGGCTGAGGAGCAGCAGTACCTGGACAAGCTGAA GCAGCTGTCGAAGTACATCGAGCCCCTGCGCCGCATGATCAACAAGATCGACAAGAACGAAGACAGAAAA AAGGACCTGAGTAAGATGAAGAGCCTTCTGGACATTCTGACAGACCCCTCGAAGCGGTGTCCCCTGAAGA CCTTGCAAAAGTGTGAGATCGCCCTGGAGAAACTCAAGAATGACATGGCGGTGCCCACTCCCCCACCGCC CCCGGTGCCACCGACCAAACAGCAGTACCTATGCCAGCCGCTCCTGGATGCCGTCCTGGCCAACATCCGC TCACCTGTCTTCAACCATTCCCTGTACCGCACATTCGTTCCAGCCATGACCGCCATTCACGGCCCACCCA TCACGGCCCCAGTGGTGTGCACCCGGAAGCGCAGGCTTGAGGATGATGAGCGGCAGAGCATCCCCAGTGT GCTCCAGGGTGAGGTGGCCAGGCTGGACCCCAAGTTCCTGGTAAACCTGGACCCTTCTCACTGCAGCAAC AATGGCACTGTCCACCTGATCTGCAAGCTGGATGACAAGGACCTCCCAAGTGTGCCACCACTGGAGCTCA GTGTGCCCGCTGACTATCCTGCCCAAAGCCCGCTGTGGATAGACCGGCAGTGGCAGTACGACGCCAACCC CTTCCTCCAGTCGGTGCACCGCTGCATGACCTCCAGGCTGCTGCAGCTCCCGGACAAGCACTCGGTCACC GCCTTGCTCAACACCTGGGCCCAGAGCGTCCACCAGGCCTGCCTCTCAGCCGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215754 representing NM_001003891
Red=Cloning site Green=Tags(s) MDVSGQETDWRSTAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIH NKKSQASVSDPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQPPPGTSGMAPHS MAVVSTATPQTQLQLQQVALQQQQQQQQFQQQQQAALQQQQQQQQQQQFQAQQSAMQQQFQAVVQQQQQL QQQQQQQQHLIKLHHQNQQQIQQQQQQLQRIAQLQLQQQQQQQQQQQQQQQQALQAQPPIQQPPMQQPQP PPSQALPQQLQQMHHTQHHQPPPQPQQPPVAQNQPSQLPPQSQTQPLVSQAQALPGQMLYTQPPLKFVRA PMVVQQPPVQPQVQQQQTAVQTAQAAQMVAPGVQMITEALAQGGMHIRARFPPTTAVSAIPSSSIPLGRQ PMAQVSQSSLPMLSSPSPGQQVQTPQSMPPPPQPSPQPGQPSSQPNSNVSSGPAPSPSSFLPSPSPQPSQ SPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQQYLDKLKQLSKYIEPLRRMINKIDKNEDRK KDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVPPTKQQYLCQPLLDAVLANIR SPVFNHSLYRTFVPAMTAIHGPPITAPVVCTRKRRLEDDERQSIPSVLQGEVARLDPKFLVNLDPSHCSN NGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPLWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHSVT ALLNTWAQSVHQACLSAA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001003891 |
ORF Size | 2364 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001003891.3 |
RefSeq Size | 3384 bp |
RefSeq ORF | 2367 bp |
Locus ID | 51586 |
UniProt ID | Q96RN5 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014] |
Documents
Product Manuals |
FAQs |
SDS |