Synaptopodin 2 (SYNPO2) (NM_133477) Human Tagged ORF Clone
CAT#: RG215728
- TrueORF®
SYNPO2 (tGFP-tagged) - Human synaptopodin 2 (SYNPO2), transcript variant 1
ORF Plasmid: DDK
"NM_133477" in other vectors (4)
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CNY 13,780.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG215728 representing NM_133477
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCACAGGGGATTTTATCTGCATTTCCATGACTGGAGGGGCGCCCTGGGGGTTCAGATTGCAAGGTG GCAAGGAGCAGAAGCAGCCCTTACAAGTTGCAAAGATTCGAAATCAGAGCAAAGCCTCTGGGTCTGGGCT CTGTGAGGGAGATGAAGTGGTTTCCATCAATGGCAACCCTTGTGCAGATCTCACCTACCCTGAAGTCATC AAGCTCATGGAAAGCATAACAGACTCTCTCCAAATGCTCATCAAAAGACCATCCAGTGGAATAAGTGAGG CTTTGATATCTGAAAATGAAAACAAAAACCTCGAGCATCTCACACATGGGGGTTATGTGGAAAGTACCAC CCTGCAGATTCGACCGGCCACAAAGACCCAGTGCACAGAATTCTTCCTCGCCCCTGTCAAGACTGAAGTT CCCCTAGCTGAGAACCAAAGAAGTGGTCCCGACTGTGCAGGCAGCTTGAAAGAAGAAACAGGCCCGAGCT ACCAAAGGGCTCCCCAAATGCCTGACTCCCAAAGAGGACGCGTGGCAGAAGAGCTGATCTTAAGGGAGAA GGTAGAAGCGGTACAGCCTGGGCCTGTGGTTGAGCTGCAACTGTCCCTTTCACAGGAGAGACATAAGGGC GCTAGTGGCCCTTTAGTGGCTCTCCCGGGAGCTGAAAAATCTAAGTCTCCTGACCCAGACCCTAACTTGT CACATGACAGGATTGTCCACATAAATTCGATCCCTACTAATGAGAAAGCAGACCCTTTCCTGAGGTCCAG CAAGATAATCCAGATCTCCAGTGGCAGAGAGTTGAGAGTGATCCAGGAAAGTGAAGCAGGAGATGCGGGA CTGCCCCGGGTGGAAGTGATCCTCGACTGCTCTGACAGGCAGAAGACAGAAGGGTGCAGGCTTCAGGCAG GAAAGGAGTGTGTGGATTCTCCAGTGGAAGGAGGGCAGTCAGAAGCACCTCCTTCTCTGGTATCCTTTGC CGTCTCATCAGAAGGCACAGAGCAGGGAGAAGATCCACGCTCGGAAAAAGATCACAGCAGACCTCACAAG CACCGAGCGCGGCATGCACGGCTCAGGAGGAGTGAAAGCCTGTCAGAAAAACAAGTGAAGGAAGCAAAAT CTAAATGCAAAAGCATTGCCCTTCTTCTAACGGATGCTCCCAGCCCCAACTCCAAGGGGGTGTTGATGTT TAAGAAGCGACGTCGGAGGGCCAGGAAATACACCCTAGTTAGCTACGGTACTGGCGAGCTTGAGCGAGAG GCGGACGAGGAGGAAGAAGGTGACAAGGAGGATACATGTGAAGTAGCATTTCTTGGTGCAAGCGAATCAG AGGTGGATGAAGAGTTATTGTCTGACGTTGACGACAACACACAAGTTGTGAACTTTGACTGGGATTCTGG ACTGGTGGACATTGAAAAGAAACTGAACAGAGGGGACAAGATGGAGATGTTACCAGACACCACAGGCAAG GGAGCCCTCATGTTTGCCAAGAGGAGGGAGAGAATGGATCAGATCACAGCCCAAAAAGAAGAGGACAAGG TAGGTGGAACGCCAAGCAGAGAACAAGATGCTGCCCAGACCGATGGCCTGAGAACCACGACTTCTTACCA AAGAAAGGAGGAAGAGTCGGTAAGAACGCAGAGCTCTGTGAGCAAAAGCTACATCGAGGTGAGTCATGGT CTTGGCCATGTTCCCCAACAGAATGGCTTCAGTGGGGCATCTGAGACAGCAAACATCCAGAGGATGGTCC CCATGAATAGAACGGCCAAACCCTTCCCAGGGTCTGTGAATCAGCCAGCTACCCCCTTCTCGCCAACCCG AAACATGACGAGTCCCATTGCTGACTTTCCTGCACCTCCACCTTACTCTGCAGTCACTCCTCCCCCTGAC GCCTTCTCCAGAGGGGTTTCAAGTCCGATTGCTGGCCCAGCACAGCCCCCTCCATGGCCCCAGCCTGCCC CGTGGTCCCAGCCAGCCTTTTACGATTCGTCTGAGCGAATAGCTTCCCGAGATGAGAGGATCTCAGTGCC AGCAAAAAGAACAGGAATATTGCAGGAGGCCAAAAGGAGAAGCACGACAAAACCCATGTTTACTTTTAAA GAGCCCAAAGTAAGCCCAAATCCTGAACTCTTGTCACTCCTTCAAAATTCAGAAGGCAAACGGGGCACTG GAGCTGGAGGTGATTCCGGACCGGAAGAAGACTACCTCAGCTTGGGGGCAGAGGCTTGTAATTTCATGCA AAGCTCCTCTGCCAAACAAAAGACCCCTCCTCCTGTTGCTCCAAAACCTGCAGTCAAGTCCTCATCCTCC CAACCAGTAACTCCAGTTTCCCCAGTCTGGTCTCCAGGAGTGGCTCCCACCCAACCTCCTGCCTTCCCCA CATCCAACCCATCAAAGGGCACCGTTGTCTCCTCCATCAAAATAGCCCAGCCTTCTTACCCTCCTGCCCG GCCTGCAAGTACTTTGAACGTGGCTGGTCCCTTCAAAGGACCACAAGCAGCAGTAGCCAGTCAGAATTAC ACACCCAAACCAACAGTTTCCACACCAACAGTCAATGCTGTTCAGCCTGGTGCAGTGGGACCATCCAATG AGCTTCCAGGAATGAGTGGGAGAGGAGCTCAGCTCTTTGCTAAAAGGCAGTCGAGAATGGAGAAGTATGT GGTCGATTCAGACACGGTGCAGGCCCACGCTGCTCGAGCTCAGTCTCCCACTCCATCTCTCCCGGCCAGT TGGAAGTACTCCTCCAATGTCCGAGCACCTCCTCCTGTGGCCTATAATCCTATCCACTCGCCGTCTTACC CACTGGCTGCTCTCAAGTCTCAGCCATCAGCTGCACAGCCCTCCAAAATGGGCAAGAAAAAGGGAAAGAA ACCCCTCAATGCATTAGATGTCATGAAGCACCAACCGTATCAGCTCAATGCATCCTTGTTTACTTTCCAA CCTCCAGATGCAAAGGATGGCCTCCCCCAGAAGTCATCAGTCAAGGTCAATTCAGCCCTGGCCATGAAGC AAGCTCTTCCTCCCCGGCCAGTGAATGCTGCCTCACCTACGAATGTGCAGGCTTCGTCAGTGTACTCGGT ACCAGCCTATACCTCTCCTCCTTCCTTCTTTGCAGAGGCCTCCTCACCAGTCAGTGCATCCCCAGTGCCT GTGGGCATTCCCACCTCGCCAAAGCAAGAATCAGCCTCATCATCTTATTTTGTGGCACCAAGGCCAAAGT TCTCAGCCAAGAAAAGTGGTGTCACAATTCAGGAGAGTGGGCGCTCCCTTTCTCTTCCTGGAAGATCAGT CCCACCCCCCATTTCTACATCTCCTTGGGTATACCAGCCTACTTATAGTTACTCTAGTAAACCAACCGAT GGACTAGAGAAAGCAAACAAGAGACCAACTCCTTGGGAAGCAGCAGCAAAGTCTCCTCTCGGTCTAGTGG ATGATGCTTTCCAACCCAGAAACATCCAGGAATCCATTGTGACAAATGTGGTTTCAGCAGCTCGGAGGAA GGTGCTTCCAGGGCCTCCAGAGGATTGGAATGAAAGACTGTCCTATATTCCTCAAACCCAGAAGGCCTAT ATGGGCTCATGTGGAAGGCAAGAGTATAATGTCACAGCCAATAATAATATGTCCACCACCTCCCAATATG GTTCACAGTTGCCATATGCATATTATAGGCAGGCTTCAAGAAATGATTCTGCAATCATGTCCATGGAAAC CAGGTCTGATTACTGTCTTCCAGTAGCTGATTACAACTACAACCCACACCCAAGGGGATGGAGACGCCAA ACA ACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215728 representing NM_133477
Red=Cloning site Green=Tags(s) MGTGDFICISMTGGAPWGFRLQGGKEQKQPLQVAKIRNQSKASGSGLCEGDEVVSINGNPCADLTYPEVI KLMESITDSLQMLIKRPSSGISEALISENENKNLEHLTHGGYVESTTLQIRPATKTQCTEFFLAPVKTEV PLAENQRSGPDCAGSLKEETGPSYQRAPQMPDSQRGRVAEELILREKVEAVQPGPVVELQLSLSQERHKG ASGPLVALPGAEKSKSPDPDPNLSHDRIVHINSIPTNEKADPFLRSSKIIQISSGRELRVIQESEAGDAG LPRVEVILDCSDRQKTEGCRLQAGKECVDSPVEGGQSEAPPSLVSFAVSSEGTEQGEDPRSEKDHSRPHK HRARHARLRRSESLSEKQVKEAKSKCKSIALLLTDAPSPNSKGVLMFKKRRRRARKYTLVSYGTGELERE ADEEEEGDKEDTCEVAFLGASESEVDEELLSDVDDNTQVVNFDWDSGLVDIEKKLNRGDKMEMLPDTTGK GALMFAKRRERMDQITAQKEEDKVGGTPSREQDAAQTDGLRTTTSYQRKEEESVRTQSSVSKSYIEVSHG LGHVPQQNGFSGASETANIQRMVPMNRTAKPFPGSVNQPATPFSPTRNMTSPIADFPAPPPYSAVTPPPD AFSRGVSSPIAGPAQPPPWPQPAPWSQPAFYDSSERIASRDERISVPAKRTGILQEAKRRSTTKPMFTFK EPKVSPNPELLSLLQNSEGKRGTGAGGDSGPEEDYLSLGAEACNFMQSSSAKQKTPPPVAPKPAVKSSSS QPVTPVSPVWSPGVAPTQPPAFPTSNPSKGTVVSSIKIAQPSYPPARPASTLNVAGPFKGPQAAVASQNY TPKPTVSTPTVNAVQPGAVGPSNELPGMSGRGAQLFAKRQSRMEKYVVDSDTVQAHAARAQSPTPSLPAS WKYSSNVRAPPPVAYNPIHSPSYPLAALKSQPSAAQPSKMGKKKGKKPLNALDVMKHQPYQLNASLFTFQ PPDAKDGLPQKSSVKVNSALAMKQALPPRPVNAASPTNVQASSVYSVPAYTSPPSFFAEASSPVSASPVP VGIPTSPKQESASSSYFVAPRPKFSAKKSGVTIQESGRSLSLPGRSVPPPISTSPWVYQPTYSYSSKPTD GLEKANKRPTPWEAAAKSPLGLVDDAFQPRNIQESIVTNVVSAARRKVLPGPPEDWNERLSYIPQTQKAY MGSCGRQEYNVTANNNMSTTSQYGSQLPYAYYRQASRNDSAIMSMETRSDYCLPVADYNYNPHPRGWRRQ T TRPLE - GFP Tag - V |
Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133477 |
ORF Size | 3783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133477.1, NP_597734.1 |
RefSeq Size | 7318 bp |
RefSeq ORF | 3786 bp |
Locus ID | 171024 |
UniProt ID | Q9UMS6 |
Gene Summary | Has an actin-binding and actin-bundling activity. Can induce the formation of F-actin networks in an isoform-specific manner (PubMed:24005909, PubMed:23225103). At the sarcomeric Z lines is proposed to act as adapter protein that links nascent myofibers to the sarcolemma via ZYX and may play a role in early assembly and stabilization of the Z lines. Involved in autophagosome formation. May play a role in chaperone-assisted selective autophagy (CASA) involved in Z lines maintenance in striated muscle under mechanical tension; may link the client-processing CASA chaperone machinery to a membrane-tethering and fusion complex providing autophagosome membranes (By similarity). Involved in regulation of cell migration (PubMed:22915763, PubMed:25883213). May be a tumor suppressor (PubMed:16885336).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215728 | SYNPO2 (Myc-DDK-tagged)-Human synaptopodin 2 (SYNPO2), transcript variant 1 |
CNY 12,540.00 |
|
RC215728L3 | Lenti-ORF clone of SYNPO2 (Myc-DDK-tagged)-Human synaptopodin 2 (SYNPO2), transcript variant 1 |
CNY 14,440.00 |
|
RC215728L4 | Lenti-ORF clone of SYNPO2 (mGFP-tagged)-Human synaptopodin 2 (SYNPO2), transcript variant 1 |
CNY 14,440.00 |
|
SC305965 | SYNPO2 (untagged)-Human synaptopodin 2 (SYNPO2), transcript variant 1 |
CNY 10,450.00 |
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