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CENPJ (NM_018451) Human Tagged ORF Clone
CAT＃: RG215682
- TrueORF®
CENPJ (tGFP-tagged) - Human centromere protein J (CENPJ)
ORF Plasmid: DDK
"NM_018451" in other vectors (6)
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CNY 10,752.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | BM032; CENP-J; CPAP; LAP; LIP1; MCPH6; Sas-4; SASS4; SCKL4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215682 representing NM_018451
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTGATGCCAACCTCTTCAGAGTTAAACAGTGGGCAGAACTTCCTAACCCAGTGGGTGACCAATC CTTCTCGGGCTGGGGTCATATTAAATCGTGGATTTCCTATTTTGGAAGCAGACAAAGAGAAGCGAGCAGC TGTGGACATTTCTACCAGCTTTCCTATTAAAGGCACACATTTTTCTGATAGCTTCAGCTTTATAAATGAA GAAGATTCACTTCTTGAAGAACAGAAGTTGGAGTCAAACAACCCTTACAAACCACAGTCAGATAAATCTG AAACCCATACAGCCTTTCCTTGCATTAAAAAGGGACCACAGGTAGCGGCATGTCACAGTGCTCCTGGACA CCAGGAAGAAAACAAAAATGACTTCATCCCAGATCTTGCGAGTGAATTCAAAGAAGGGGCTTATAAAGAC CCACTTTTTAAAAAACTTGAACAGCTGAAAGAAGTACAACAGAAGAAGCAGGAACAATTGAAGAGGCAAC AGTTGGAGCAACTACAGAGACTCATGGAAGAACAAGAGAAGCTGCTCACCATGGTGTCTGGGCAGTGCAC ACTTCCAGGTTTGAGTTTACTGCCTGATGATCAGAGCCAGAAGCACAGGTCTCCAGGAAATACCACCACT GGAGAGAGAGCCACATGCTGCTTCCCATCATATGTCTACCCGGACCCAACCCAGGAAGAAACATACCCGT CCAACATTTTATCCCATGAGCAAAGCAACTTCTGTAGAACTGCTCATGGAGATTTTGTCTTAACTTCAAA ACGTGCGTCTCCTAATTTATTTTCTGAGGCACAGTATCAAGAAGCACCTGTGGAAAAAAATAATTTAAAA GAAGAAAACCGTAACCATCCTACAGGAGAAAGTATCTTATGTTGGGAGAAAGTGACAGAACAGATTCAGG AAGCAAATGATAAGAACTTACAAAAACATGATGATTCCTCAGAAGTGGCTAATATTGAAGAAAGGCCCAT TAAAGCTGCTATTGGAGAAAGGAAACAGACCTTTGAAGATTACTTAGAAGAACAAATTCAGTTGGAAGAG CAAGAACTGAAGCAAAAACAGCTGAAGGAAGCAGAAGGACCATTGCCAATCAAAGCAAAACCAAAACAAC CATTTTTAAAACGAGGAGAAGGTTTAGCTAGATTTACTAATGCCAAATCTAAGTTTCAAAAAGGCAAAGA AAGTAAACTAGTGACTAACCAGAGCACTTCCGAGGACCAGCCGCTGTTTAAAATGGATAGACAGCAACTC CAGCGGAAAACCGCTCTTAAAAATAAAGAGCTGTGTGCAGACAACCCTATCCTTAAAAAGGACAGTAAAG CTAGAACCAAGAGTGGTTCTGTCACCCTCAGTCAGAAGCCGAAAATGCTGAAGTGTAGTAACAGGAAAAG TCTTTCTCCGTCAGGATTGAAAATACAGACGGGGAAGAAATGTGATGGGCAGTTTAGAGACCAGATCAAA TTTGAAAACAAAGTCACATCTAATAATAAAGAAAATGTAACTGAGTGTCCAAAACCTTGCGATACTGGCT GCACAGGGTGGAATAAGACACAAGGTAAAGACAGACTTCCTCTTTCAACAGGGCCGGCCAGCCGGCTGGC TGCTAAGAGCCCCATAAGGGAGACCATGAAAGAGTCTGAATCTTCTCTTGACGTTTCTCTTCAGAAAAAG TTAGAGACTTGGGAACGAGAAAAGGAAAAGGAAAATTTGGAATTAGATGAATTTTTGTTTTTAGAACAAG CTGCTGATGAAATATCATTTTCTAGTAATTCCTCATTTGTACTGAAAATCTTAGAAAGGGATCAACAGAT CTGCAAAGGTCACCGGATGTCTTCCACCCCTGTCAAAGCTGTGCCACAGAAGACAAATCCGGCAGATCCC ATTAGTCATTGTAACCGCAGTGAGGATTTGGACCACACTGCACGTGAGAAGGAGAGTGAGTGTGAAGTCG CACCCAAACAACTTCATTCATTGTCCTCAGCTGATGAATTGAGGGAACAGCCTTGTAAAATCAGGAAAGC CGTCCAAAAGAGCACTTCTGAAAATCAGACTGAATGGAATGCACGTGACGATGAAGGTGTTCCAAATAGT GACAGTAGCACTGACTCTGAGGAACAGCTTGATGTTACCATAAAACCATCGACTGAGGATAGAGAGAGGG GCATCAGCAGCAGAGAGGATAGCCCACAAGTCTGTGATGATAAGGGGCCTTTTAAGGACACCAGGACCCA AGAAGATAAAAGGAGAGATGTTGATCTGGATTTGTCTGATAAAGATTACAGTAGCGATGAGTCTATCATG GAAAGCATAAAACATAAAGTGTCTGAGCCCTCGAGATCCTCATCCCTAAGTCTGAGTAAAATGGACTTTG ATGATGAAAGAACTTGGACTGACCTTGAAGAGAATTTGTGTAACCATGATGTTGTTCTTGGGAATGAATC CACTTATGGGACGCCGCAGACATGCTACCCTAATAATGAAATAGGTATCCTGGACAAAACAATAAAAAGG AAGATTGCACCAGTCAAGAGGGGAGAAGACTTGAGCAAGTCCAGGAGGAGCAGAAGTCCTCCTACATCGG AGCTGATGATGAAATTCTTTCCTTCTTTGAAACCAAAACCAAAGTCAGATTCACACTTGGGAAATGAACT CAAGTTAAACATAAGTCAAGACCAACCACCTGGTGACAATGCTCGATCCCAGGTTTTGAGAGAGAAAATT ATTGAATTGGAAACAGAAATAGAAAAGTTTAAAGCTGAGAACGCATCTTTAGCTAAACTTCGCATTGAAC GAGAAAGTGCCTTGGAAAAACTCAGGAAAGAAATTGCAGACTTCGAACAACAGAAAGCAAAAGAATTAGC TCGAATAGAAGAGTTTAAAAAGGAGGAGATGAGGAAGCTACAAAAGGAACGTAAAGTTTTTGAAAAGTAT ACTACAGCTGCAAGAACTTTTCCAGATAAAAAGGAACGTGAAGAAATACAGACTTTAAAACAGCAAATAG CAGATTTACGGGAAGATTTGAAAAGAAAGGAGACCAAATGGTCAAGTACACACAGCCGTCTCAGAAGCCA GATACAAATGTTAGTCAGAGAGAACACAGACCTCCGGGAAGAAATAAAAGTGATGGAAAGATTCCGACTG GATGCCTGGAAGAGAGCAGAAGCCATAGAGAGCAGCCTCGAGGTGGAGAAGAAGGACAAGCTTGCGAACA CATCTGTTCGATTTCAAAACAGTCAGATTTCTTCAGGAACCCAGGTAGAAAAATACAAGAAAAATTATCT TCCAATGCAAGGCAATCCACCTCGAAGATCCAAGTCTGCACCTCCTCGTGATTTAGGCAATTTGGATAAG GGACAAGCTGCCTCTCCCAGGGAGCCACTTGAACCACTGAACTTCCCAGATCCTGAATATAAAGAGGAGG AGGAAGACCAAGACATACAGGGAGAAATCAGTCATCCTGATGGAAAGGTGGAAAAGGTTTATAAGAATGG GTGCCGTGTTATACTGTTTCCCAATGGAACTCGAAAGGAAGTGAGTGCAGATGGGAAGACCATCACTGTC ACTTTCTTTAATGGTGACGTGAAGCAGGTCATGCCAGACCAAAGAGTGATCTACTACTATGCAGCTGCCC AGACCACTCACACGACATACCCGGAGGGACTGGAAGTCTTACATTTCTCAAGTGGACAAATAGAAAAACA TTACCCAGATGGAAGAAAAGAAATCACGTTTCCTGACCAGACTGTTAAAAACTTATTTCCTGATGGACAA GAAGAAAGCATTTTCCCAGATGGTACAATTGTCAGAGTACAACGTGATGGCAACAAACTCATAGAGTTTA ATAATGGCCAAAGAGAACTACATACTGCCCAGTTCAAGAGACGGGAATACCCAGATGGCACTGTTAAAAC CGTATATGCAAACGGTCATCAAGAAACGAAGTACAGATCCGGTCGGATAAGAGTTAAGGACAAGGAGGGT AATGTGCTAATGGACACGGAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215682 representing NM_018451
Red=Cloning site Green=Tags(s) MFLMPTSSELNSGQNFLTQWVTNPSRAGVILNRGFPILEADKEKRAAVDISTSFPIKGTHFSDSFSFINE EDSLLEEQKLESNNPYKPQSDKSETHTAFPCIKKGPQVAACHSAPGHQEENKNDFIPDLASEFKEGAYKD PLFKKLEQLKEVQQKKQEQLKRQQLEQLQRLMEEQEKLLTMVSGQCTLPGLSLLPDDQSQKHRSPGNTTT GERATCCFPSYVYPDPTQEETYPSNILSHEQSNFCRTAHGDFVLTSKRASPNLFSEAQYQEAPVEKNNLK EENRNHPTGESILCWEKVTEQIQEANDKNLQKHDDSSEVANIEERPIKAAIGERKQTFEDYLEEQIQLEE QELKQKQLKEAEGPLPIKAKPKQPFLKRGEGLARFTNAKSKFQKGKESKLVTNQSTSEDQPLFKMDRQQL QRKTALKNKELCADNPILKKDSKARTKSGSVTLSQKPKMLKCSNRKSLSPSGLKIQTGKKCDGQFRDQIK FENKVTSNNKENVTECPKPCDTGCTGWNKTQGKDRLPLSTGPASRLAAKSPIRETMKESESSLDVSLQKK LETWEREKEKENLELDEFLFLEQAADEISFSSNSSFVLKILERDQQICKGHRMSSTPVKAVPQKTNPADP ISHCNRSEDLDHTAREKESECEVAPKQLHSLSSADELREQPCKIRKAVQKSTSENQTEWNARDDEGVPNS DSSTDSEEQLDVTIKPSTEDRERGISSREDSPQVCDDKGPFKDTRTQEDKRRDVDLDLSDKDYSSDESIM ESIKHKVSEPSRSSSLSLSKMDFDDERTWTDLEENLCNHDVVLGNESTYGTPQTCYPNNEIGILDKTIKR KIAPVKRGEDLSKSRRSRSPPTSELMMKFFPSLKPKPKSDSHLGNELKLNISQDQPPGDNARSQVLREKI IELETEIEKFKAENASLAKLRIERESALEKLRKEIADFEQQKAKELARIEEFKKEEMRKLQKERKVFEKY TTAARTFPDKKEREEIQTLKQQIADLREDLKRKETKWSSTHSRLRSQIQMLVRENTDLREEIKVMERFRL DAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDK GQAASPREPLEPLNFPDPEYKEEEEDQDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITV TFFNGDVKQVMPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQ EESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRSGRIRVKDKEG NVLMDTEL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018451 |
| ORF Size | 4014 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018451.2, NP_060921.2 |
| RefSeq Size | 4387 bp |
| RefSeq ORF | 4017 bp |
| Locus ID | 55835 |
| UniProt ID | Q9HC77 |
| Gene Summary | This gene encodes a protein that belongs to the centromere protein family. During cell division, this protein plays a structural role in the maintenance of centrosome integrity and normal spindle morphology, and it is involved in microtubule disassembly at the centrosome. This protein can function as a transcriptional coactivator in the Stat5 signaling pathway, and also as a coactivator of NF-kappaB-mediated transcription, likely via its interaction with the coactivator p300/CREB-binding protein. Mutations in this gene are associated with primary autosomal recessive microcephaly, a disorder characterized by severely reduced brain size and cognitive disability. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215682 | CENPJ (Myc-DDK-tagged)-Human centromere protein J (CENPJ) |
CNY 9,152.00 |
|
| RC215682L1 | Lenti ORF clone of Human centromere protein J (CENPJ), Myc-DDK-tagged |
CNY 11,552.00 |
|
| RC215682L2 | Lenti ORF clone of Human centromere protein J (CENPJ), mGFP tagged |
CNY 15,200.00 |
|
| RC215682L3 | Lenti ORF clone of Human centromere protein J (CENPJ), Myc-DDK-tagged |
CNY 15,200.00 |
|
| RC215682L4 | Lenti ORF clone of Human centromere protein J (CENPJ), mGFP tagged |
CNY 15,200.00 |
|
| SC308859 | CENPJ (untagged)-Human centromere protein J (CENPJ) |
CNY 9,160.00 |
