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Claspin (CLSPN) (NM_022111) Human Tagged ORF Clone
CAT＃: RG215378
- TrueORF®
CLSPN (tGFP-tagged) - Human claspin (CLSPN), transcript variant 1
ORF Plasmid: DDK
"NM_022111" in other vectors (4)
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CNY 14,730.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215378 representing NM_022111
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGGCGAGGTGGGTTCTGAGGTTCACCTAGAAATCAATGACCCAAACGTCATTTCACAAGAGGAAG CAGATAGTCCTTCAGATAGTGGACAGGGCAGCTATGAAACAATTGGACCCTTGAGTGAAGGAGATTCAGA TGAAGAGATATTTGTAAGTAAGAAGTTGAAAAACAGGAAGGTTCTACAAGACAGTGATTCCGAAACAGAG GACACAAATGCCTCTCCAGAGAAAACTACCTATGACAGTGCCGAGGAGGAAAATAAAGAGAATTTATATG CTGGGAAAAATACAAAAATCAAAAGGATTTACAAAACTGTGGCAGACAGTGATGAAAGTTACATGGAAAA GTCTTTGTATCAGGAAAATCTTGAAGCGCAAGTGAAACCTTGCTTAGAGCTGAGTCTTCAGTCTGGAAAC TCTACAGACTTTACCACTGACAGAAAGAGTTCCAAAAAGCACATACATGATAAAGAAGGAACTGCAGGAA AAGCAAAAGTAAAATCAAAAAGAAGACTTGAGAAAGAGGAGAGAAAAATGGAAAAAATTAGACAGCTAAA AAAGAAGGAAACAAAAAACCAGGAAGATGATGTAGAACAGCCATTTAATGACAGTGGCTGTCTTCTTGTG GATAAAGACCTTTTTGAAACTGGGTTGGAGGATGAAAATAACTCTCCATTGGAAGATGAAGAGTCATTAG AATCAATAAGAGCAGCTGTAAAAAACAAAGTAAAAAAGCACAAGAAAAAAGAACCATCTTTGGAGAGTGG GGTCCATTCATTTGAGGAAGGAAGTGAGTTATCAAAAGGAACCACGAGGAAGGAAAGAAAGGCAGCCAGA TTAAGTAAAGAAGCATTAAAACAACTGCATAGTGAGACTCAGCGCCTTATTCGAGAGTCTGCACTGAACC TTCCATATCATATGCCTGAGAATAAAACCATTCATGATTTCTTCAAACGTAAACCCCGGCCCACTTGCCA CGGAAATGCCATGGCACTATTGAAGTCATCTAAATATCAGTCAAGCCATCACAAAGAAATCATAGACACT GCAAATACTACTGAAATGAACAGTGATCACCATAGTAAAGGTTCTGAGCAGACAACAGGTGCAGAAAATG AAGTGGAAACTAATGCACTCCCTGTAGTTTCAAAGGAAACCCAGATCATTACTGGATCAGATGAGTCTTG CAGGAAGGATTTGGTAAAAAATGAAGAGCTAGAAATTCAGGAGAAACAGAAGCAGAGTGACATTAGACCT TCACCTGGGGACAGCTCAGTGTTGCAACAGGAATCCAACTTCCTCGGGAACAATCACAGTGAGGAATGTC AGGTTGGAGGGCTTGTAGCATTTGAACCTCATGCCCTGGAGGGTGAAGGCCCCCAAAATCCAGAAGAAAC AGATGAGAAAGTGGAAGAGCCTGAGCAGCAAAATAAATCATCAGCAGTTGGGCCACCTGAAAAAGTGAGA CGGTTTACTCTGGATAGACTTAAGCAACTGGGAGTAGATGTTTCCATTAAACCACGGCTAGGTGCTGATG AAGATTCCTTTGTGATACTTGAACCTGAAACCAACAGAGAACTGGAAGCCTTGAAGCAGCGTTTCTGGAA GCATGCTAATCCAGCAGCCAAACCCAGGGCTGGTCAGACAGTGAATGTGAACGTCATAGTGAAAGACATG GGCACTGATGGAAAGGAAGAGCTAAAAGCAGATGTGGTACCTGTGACTTTAGCACCTAAGAAGTTGGATG GAGCAAGCCACACAAAACCAGGTGAAAAGCTTCAGGTGTTAAAAGCTAAACTGCAAGAAGCAATGAAACT CCGAAGGTTTGAGGAGCGCCAGAAGCGCCAAGCACTGTTTAAATTAGATAATGAAGATGGGTTTGAGGAA GAGGAGGAGGAAGAGGAAGAAATGACAGATGAGTCTGAGGAAGATGGAGAAGAGAAGGTAGAGAAAGAAG AGAAAGAGGAAGAACTAGAGGAAGAGGAGGAGAAAGAAGAGGAGGAGGAAGAAGAAGGAAATCAGGAGAC TGCAGAATTCCTTCTTAGTAGTGAAGAAATAGAAACAAAAGATGAAAAAGAAATGGATAAAGAAAATAAT GATGGCAGTAGTGAAATTGGCAAGGCAGTTGGCTTCCTCTCTGTTCCCAAGTCTCTCTCATCAGATTCTA CTTTACTTCTGTTTAAGGACAGCTCTTCCAAGATGGGTTACTTTCCTACTGAAGAAAAATCAGAAACAGA TGAAAACTCAGGCAAGCAGCCTAGCAAACTGGATGAGGATGATTCATGTTCATTGCTAACAAAGGAGAGC AGCCACAATAGCAGCTTTGAGCTGATTGGCTCCACGATTCCATCCTATCAGCCTTGCAACAGACAAACAG GCCGTGGGACCAGTTTTTTCCCTACAGCAGGAGGATTCAGATCTCCTTCCCCTGGGCTATTTCGAGCCAG TTTGGTCAGCTCAGCTTCTAAGAGTTCAGGGAAACTGTCTGAGCCTTCACTTCCCATAGAGGATTCCCAG GATCTGTATAACGCCTCCCCAGAGCCTAAGACACTTTTCCTAGGAGCAGGAGACTTCCAGTTCTGTTTAG AAGATGACACTCAGAGCCAACTGTTGGATGCAGATGGGTTCTTAAATGTTAGAAACCACAGGAATCAGTA CCAAGCTTTGAAGCCTCGATTGCCATTGGCCAGTATGGATGAGAATGCCATGGATGCCAACATGGATGAG CTGTTGGATTTGTGTACTGGAAAGTTCACATCTCAGGCTGAAAAACATCTACCCAGGAAGAGTGACAAGA AAGAGAACATGGAGGAACTTCTGAACCTTTGTTCAGGAAAATTCACTTCTCAGGATGCCTCCACTCCAGC CTCATCAGAGTTAAATAAACAGGAGAAGGAGAGCAGCATGGGTGATCCAATGGAAGAAGCACTTGCTCTT TGCTCAGGCTCTTTTCCCACAGACAAGGAAGAGGAAGACGAGGAGGAGGAATTTGGAGACTTTCGGCTTG TTTCAAATGATAATGAGTTTGATAGTGATGAGGATGAACACAGTGACTCTGGTAATGATCTGGCACTGGA AGACCATGAAGATGATGATGAAGAAGAACTCCTGAAGCGATCTGAGAAGTTGAAAAGGCAAATGAGGTTG AGGAAATACCTGGAGGATGAGGCAGAGGTGTCAGGAAGTGATGTGGGAAGCGAAGATGAGTATGATGGGG AAGAAATTGATGAATATGAAGAGGACGTAATTGATGAAGTACTTCCTTCTGATGAGGAACTGCAGAGTCA AATCAAGAAAATACACATGAAAACTATGTTGGATGATGATAAGCGACAGCTACGTTTATACCAAGAGAGG TACCTTGCTGATGGGGATCTGCACAGCGATGGTCCTGGGCGAATGAGGAAGTTTCGATGGAAAAACATAG ATGATGCTTCCCAGATGGACTTGTTCCACAGAGACTCTGATGATGATCAGACTGAAGAACAGCTTGATGA GTCAGAAGCCAGGTGGAGGAAGGAGCGAATTGAACGAGAGCAGTGGCTTCGGGACATGGCACAGCAGGGG AAAATTACAGCTGAAGAAGAAGAAGAAATTGGGGAGGACAGTCAGTTTATGATACTGGCCAAGAAAGTTA CAGCCAAAGCACTGCAGAAGAATGCCAGTCGCCCTATGGTTATTCAGGAATCAAAGTCTTTGCTCAGAAA TCCTTTTGAAGCCATCAGACCAGGAAGTGCTCAACAGGTGAAGACAGGCTCACTGCTAAACCAGCCCAAA GCTGTGCTTCAGAAACTGGCTGCTCTCTCTGACCATAACCCCAGTGCTCCTCGAAATTCAAGAAACTTTG TCTTTCATACACTTTCTCCTGTCAAGGCTGAGGCGGCAAAGGAATCGTCTAAGTCTCAGGTAAAGAAAAG GGGTCCATCTTTCATGACTTCTCCTTCACCTAAGCACCTCAAAACAGATGATAGCACTTCAGGATTGACG CGAAGCATCTTCAAATATTTGGAGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215378 representing NM_022111
Red=Cloning site Green=Tags(s) MTGEVGSEVHLEINDPNVISQEEADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETE DTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQENLEAQVKPCLELSLQSGN STDFTTDRKSSKKHIHDKEGTAGKAKVKSKRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLV DKDLFETGLEDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTTRKERKAAR LSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKPRPTCHGNAMALLKSSKYQSSHHKEIIDT ANTTEMNSDHHSKGSEQTTGAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRP SPGDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDEKVEEPEQQNKSSAVGPPEKVR RFTLDRLKQLGVDVSIKPRLGADEDSFVILEPETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDM GTDGKEELKADVVPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQALFKLDNEDGFEE EEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEEGNQETAEFLLSSEEIETKDEKEMDKENN DGSSEIGKAVGFLSVPKSLSSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKES SHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSASKSSGKLSEPSLPIEDSQ DLYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDE LLDLCTGKFTSQAEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGDPMEEALAL CSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGNDLALEDHEDDDEEELLKRSEKLKRQMRL RKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQER YLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARWRKERIEREQWLRDMAQQG KITAEEEEEIGEDSQFMILAKKVTAKALQKNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPK AVLQKLAALSDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKTDDSTSGLT RSIFKYLES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022111 |
| ORF Size | 4017 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022111.4 |
| RefSeq Size | 8484 bp |
| RefSeq ORF | 4020 bp |
| Locus ID | 63967 |
| UniProt ID | Q9HAW4 |
| Gene Summary | The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215378 | CLSPN (Myc-DDK-tagged)-Human claspin (CLSPN), transcript variant 1 |
CNY 10,080.00 |
|
| RC215378L3 | Lenti-ORF clone of CLSPN (Myc-DDK-tagged)-Human claspin (CLSPN), transcript variant 1 |
CNY 12,480.00 |
|
| RC215378L4 | Lenti-ORF clone of CLSPN (mGFP-tagged)-Human claspin (CLSPN), transcript variant 1 |
CNY 15,300.00 |
|
| SC304993 | CLSPN (untagged)-Human claspin (CLSPN), transcript variant 1 |
CNY 9,168.00 |
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