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Factor V (F5) (NM_000130) Human Tagged ORF Clone
CAT＃: RG215231
- TrueORF®
F5 (tGFP-tagged) - Human coagulation factor V (proaccelerin, labile factor) (F5)
ORF Plasmid: DDK
"NM_000130" in other vectors (2)
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CNY 20,900.00
货期*
4周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FVL; PCCF; RPRGL1; THPH2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215231 representing NM_000130
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCAGGCTGCCCACGCCTCTGGGTCCTGGTGGTCTTGGGCACCAGCTGGGTAGGCTGGGGGAGCC AAGGGACAGAAGCGGCACAGCTAAGGCAGTTCTACGTGGCTGCTCAGGGCATCAGTTGGAGCTACCGACC TGAGCCCACAAACTCAAGTTTGAATCTTTCTGTAACTTCCTTTAAGAAAATTGTCTACAGAGAGTATGAA CCATATTTTAAGAAAGAAAAACCACAATCTACCATTTCAGGACTTCTTGGGCCTACTTTATATGCTGAAG TCGGAGACATCATAAAAGTTCACTTTAAAAATAAGGCAGATAAGCCCTTGAGCATCCATCCTCAAGGAAT TAGGTACAGTAAATTATCAGAAGGTGCTTCTTACCTTGACCACACATTCCCTGCAGAGAAGATGGACGAC GCTGTGGCTCCAGGCCGAGAATACACCTATGAATGGAGTATCAGTGAGGACAGTGGACCCACCCATGATG ACCCTCCATGCCTCACACACATCTATTACTCCCATGAAAATCTGATCGAGGATTTCAACTCTGGGCTGAT TGGGCCCCTGCTTATCTGTAAAAAAGGGACCCTAACTGAGGGTGGGACACAGAAGACGTTTGACAAGCAA ATCGTGCTACTATTTGCTGTGTTTGATGAAAGCAAGAGCTGGAGCCAGTCATCATCCCTAATGTACACAG TCAATGGATATGTGAATGGGACAATGCCAGATATAACAGTTTGTGCCCATGACCACATCAGCTGGCATCT GCTGGGAATGAGCTCGGGGCCAGAATTATTCTCCATTCATTTCAACGGCCAGGTCCTGGAGCAGAACCAT CATAAGGTCTCAGCCATCACCCTTGTCAGTGCTACATCCACTACCGCAAATATGACTGTGGGCCCAGAGG GAAAGTGGATCATATCTTCTCTCACCCCAAAACATTTGCAAGCTGGGATGCAGGCTTACATTGACATTAA AAACTGCCCAAAGAAAACCAGGAATCTTAAGAAAATAACTCGTGAGCAGAGGCGGCACATGAAGAGGTGG GAATACTTCATTGCTGCAGAGGAAGTCATTTGGGACTATGCACCTGTAATACCAGCGAATATGGACAAAA AATACAGGTCTCAGCATTTGGATAATTTCTCAAACCAAATTGGAAAACATTATAAGAAAGTTATGTACAC ACAGTACGAAGATGAGTCCTTCACCAAACATACAGTGAATCCCAATATGAAAGAAGATGGGATTTTGGGT CCTATTATCAGAGCCCAGGTCAGAGACACACTCAAAATCGTGTTCAAAAATATGGCCAGCCGCCCCTATA GCATTTACCCTCATGGAGTGACCTTCTCGCCTTATGAAGATGAAGTCAACTCTTCTTTCACCTCAGGCAG GAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTATAAGTGGAACATCTTAGAGTTT GATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTACAGTGACGTGGACATCATGAGAG ACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGATCCCTGGACAGGCGAGGAATACA GAGGGCAGCAGACATCGAACAGCAGGCTGTGTTTGCTGTGTTTGATGAGAACAAAAGCTGGTACCTTGAG GACAACATCAACAAGTTTTGTGAAAATCCTGATGAGGTGAAACGTGATGACCCCAAGTTTTATGAATCAA ACATCATGAGCACTATCAATGGCTATGTGCCTGAGAGCATAACTACTCTTGGATTCTGCTTTGATGACAC TGTCCAGTGGCACTTCTGTAGTGTGGGGACCCAGAATGAAATTTTGACCATCCACTTCACTGGGCACTCA TTCATCTATGGAAAGAGGCATGAGGACACCTTGACCCTCTTCCCCATGCGTGGAGAATCTGTGACGGTCA CAATGGATAATGTTGGAACTTGGATGTTAACTTCCATGAATTCTAGTCCAAGAAGCAAAAAGCTGAGGCT GAAATTCAGGGATGTTAAATGTATCCCAGATGATGATGAAGACTCATATGAGATTTTTGAACCTCCAGAA TCTACAGTCATGGCTACACGGAAAATGCATGATCGTTTAGAACCTGAAGATGAAGAGAGTGATGCTGACT ATGATTACCAGAACAGACTGGCTGCAGCATTAGGAATTAGGTCATTCCGAAACTCATCATTGAACCAGGA AGAAGAAGAGTTCAATCTTACTGCCCTAGCTCTGGAGAATGGCACTGAATTCGTTTCTTCGAACACAGAT ATAATTGTTGGTTCAAATTATTCTTCCCCAAGTAATATTAGTAAGTTCACTGTCAATAACCTTGCAGAAC CTCAGAAAGCCCCTTCTCACCAACAAGCCACCACAGCTGGTTCCCCACTGAGACACCTCATTGGCAAGAA CTCAGTTCTCAATTCTTCCACAGCAGAGCATTCCAGCCCATATTCTGAAGACCCTATAGAGGATCCTCTA CAGCCAGATGTCACAGGGATACGTCTACTTTCACTTGGTGCTGGAGAATTCAGAAGTCAAGAACATGCTA AGCGTAAGGGACCCAAGGTAGAAAGAGATCAAGCAGCAAAGCACAGGTTCTCCTGGATGAAATTACTAGC ACATAAAGTTGGGAGACACCTAAGCCAAGACACTGGTTCTCCTTCCGGAATGAGGCCCTGGGAGGACCTT CCTAGCCAAGACACTGGTTCTCCTTCCAGAATGAGGCCCTGGGAGGACCCTCCTAGTGATCTGTTACTCT TAAAACAAAGTAACTCATCTAAGATTTTGGTTGGGAGATGGCATTTGGCTTCTGAGAAAGGTAGCTATGA AATAATCCAAGATACTGATGAAGACACAGCTGTTAACAATTGGCTGATCAGCCCCCAGAATGCCTCACGT GCTTGGGGAGAAAGCACCCCTCTTGCCAACAAGCCTGGAAAGCAGAGTGGCCACCCAAAGTTTCCTAGAG TTAGACATAAATCTCTACAAGTAAGACAGGATGGAGGAAAGAGTAGACTGAAGAAAAGCCAGTTTCTCAT TAAGACACGAAAAAAGAAAAAAGAGAAGCACACACACCATGCTCCTTTATCTCCGAGGACCTTTCACCCT CTAAGAAGTGAAGCCTACAACACATTTTCAGAAAGAAGACTTAAGCATTCGTTGGTGCTTCATAAATCCA ATGAAACATCTCTTCCCACAGACCTCAATCAGACATTGCCCTCTATGGATTTTGGCTGGATAGCCTCACT TCCTGACCATAATCAGAATTCCTCAAATGACACTGGTCAGGCAAGCTGTCCTCCAGGTCTTTATCAGACA GTGCCCCCAGAGGAACACTATCAAACATTCCCCATTCAAGACCCTGATCAAATGCACTCTACTTCAGACC CCAGTCACAGATCCTCTTCTCCAGAGCTCAGTGAAATGCTTGAGTATGACCGAAGTCACAAGTCCTTCCC CACAGATATAAGTCAAATGTCCCCTTCCTCAGAACATGAAGTCTGGCAGACAGTCATCTCTCCAGACCTC AGCCAGGTGACCCTCTCTCCAGAACTCAGCCAGACAAACCTCTCTCCAGACCTCAGCCACACGACTCTCT CTCCAGAACTCATTCAGAGAAACCTTTCCCCAGCCCTCGGTCAGATGCCCATTTCTCCAGACCTCAGCCA TACAACCCTTTCTCCAGACCTCAGCCATACAACCCTTTCTTTAGACCTCAGCCAGACAAACCTCTCTCCA GAACTCAGTCAGACAAACCTTTCTCCAGCCCTCGGTCAGATGCCCCTTTCTCCAGACCTCAGCCATACAA CCATTTCTCTAGACTTCAGCCAGACAAACCTCTCTCCAGAACTCAGCCATATGACTCTCTCTCCAGAACT CAGTCAGACAAACCTTTCCCCAGCCCTCGGTCAGATGCCCATTTCTCCAGACCTCAGCCATACAACCCTT TCTCTAGACTTCAGCCAGACAAACCTCTCTCCAGAACTCAGTCAAACAAACCTTTCCCCAGCCCTCGGTC AGATGCCCCTTTCTCCAGACCCCAGCCATACAACCCTTTCTCTAGACCTCAGCCAGACAAACCTCTCTCC AGAACTCAGTCAGACAAACCTTTCCCCAGACCTCAGTGAGATGCCCCTCTTTGCAGATCTCAGTCAAATT CCCCTTACCCCAGACCTCGACCAGATGACACTTTCTCCAGACCTTGGTGAGACAGATCTTTCCCCAAACT TTGGTCAGATGTCCCTTTCCCCAGACCTCAGCCAGGTGACTCTCTCTCCAGACATCAGTGACACCACCCT TCTCCCGGATCTCAGCCAGATATCACCTCCTCCAGACCTTGATCAGATATTCTACCCTTCTGAATCTAGT CAGTCATTGCTTCTTCAAGAATTTAATGAGTCTTTTCCTTATCCAGACCTTGGTCAGATGCCATCTCCTT CATCTCCTACTCTCAATGATACTTTTCTATCAAAGGAATTTAATCCACTGGTTATAGTGGGCCTCAGTAA AGATGGTACAGATTACATTGAGATCATTCCAAAGGAAGAGGTCCAGAGCAGTGAAGATGACTATGCTGAA ATTGATTATGTGCCCTATGATGACCCCTACAAAACTGATGTTAGGACAAACATCAACTCCTCCAGAGATC CTGACAACATTGCAGCATGGTACCTCCGCAGCAACAATGGAAACAGAAGAAATTATTACATTGCTGCTGA AGAAATATCCTGGGATTATTCAGAATTTGTACAAAGGGAAACAGATATTGAAGACTCTGATGATATTCCA GAAGATACCACATATAAGAAAGTAGTTTTTCGAAAGTACCTCGACAGCACTTTTACCAAACGTGATCCTC GAGGGGAGTATGAAGAGCATCTCGGAATTCTTGGTCCTATTATCAGAGCTGAAGTGGATGATGTTATCCA AGTTCGTTTTAAAAATTTAGCATCCAGACCGTATTCTCTACATGCCCATGGACTTTCCTATGAAAAATCA TCAGAGGGAAAGACTTATGAAGATGACTCTCCTGAATGGTTTAAGGAAGATAATGCTGTTCAGCCAAATA GCAGTTATACCTACGTATGGCATGCCACTGAGCGATCAGGGCCAGAAAGTCCTGGCTCTGCCTGTCGGGC TTGGGCCTACTACTCAGCTGTGAACCCAGAAAAAGATATTCACTCAGGCTTGATAGGTCCCCTCCTAATC TGCCAAAAAGGAATACTACATAAGGACAGCAACATGCCTGTGGACATGAGAGAATTTGTCTTACTATTTA TGACCTTTGATGAAAAGAAGAGCTGGTACTATGAAAAGAAGTCCCGAAGTTCTTGGAGACTCACATCCTC AGAAATGAAAAAATCCCATGAGTTTCACGCCATTAATGGGATGATCTACAGCTTGCCTGGCCTGAAAATG TATGAGCAAGAGTGGGTGAGGTTACACCTGCTGAACATAGGCGGCTCCCAAGACATTCACGTGGTTCACT TTCACGGCCAGACCTTGCTGGAAAATGGCAATAAACAGCACCAGTTAGGGGTCTGGCCCCTTCTGCCTGG TTCATTTAAAACTCTTGAAATGAAGGCATCAAAACCTGGCTGGTGGCTCCTAAACACAGAGGTTGGAGAA AACCAGAGAGCAGGGATGCAAACGCCATTTCTTATCATGGACAGAGACTGTAGGATGCCAATGGGACTAA GCACTGGTATCATATCTGATTCACAGATCAAGGCTTCAGAGTTTCTGGGTTACTGGGAGCCCAGATTAGC AAGATTAAACAATGGTGGATCTTATAATGCTTGGAGTGTAGAAAAACTTGCAGCAGAATTTGCCTCTAAA CCTTGGATCCAGGTGGACATGCAAAAGGAAGTCATAATCACAGGGATCCAGACCCAAGGTGCCAAACACT ACCTGAAGTCCTGCTATACCACAGAGTTCTATGTAGCTTACAGTTCCAACCAGATCAACTGGCAGATCTT CAAAGGGAACAGCACAAGGAATGTGATGTATTTTAATGGCAATTCAGATGCCTCTACAATAAAAGAGAAT CAGTTTGACCCACCTATTGTGGCTAGATATATTAGGATCTCTCCAACTCGAGCCTATAACAGACCTACCC TTCGATTGGAACTGCAAGGTTGTGAGGTAAATGGATGTTCCACACCCCTGGGTATGGAAAATGGAAAGAT AGAAAACAAGCAAATCACAGCTTCTTCGTTTAAGAAATCTTGGTGGGGAGATTACTGGGAACCCTTCCGT GCCCGTCTGAATGCCCAGGGACGTGTGAATGCCTGGCAAGCCAAGGCAAACAACAATAAGCAGTGGCTAG AAATTGATCTACTCAAGATCAAGAAGATAACGGCAATTATAACACAGGGCTGCAAGTCTCTGTCCTCTGA AATGTATGTAAAGAGCTATACCATCCACTACAGTGAGCAGGGAGTGGAATGGAAACCATACAGGCTGAAA TCCTCCATGGTGGACAAGATTTTTGAAGGAAATACTAATACCAAAGGACATGTGAAGAACTTTTTCAACC CCCCAATCATTTCCAGGTTTATCCGTGTCATTCCTAAAACATGGAATCAAAGTATTGCACTTCGCCTGGA ACTCTTTGGCTGTGATATTTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215231 representing NM_000130
Red=Cloning site Green=Tags(s) MFPGCPRLWVLVVLGTSWVGWGSQGTEAAQLRQFYVAAQGISWSYRPEPTNSSLNLSVTSFKKIVYREYE PYFKKEKPQSTISGLLGPTLYAEVGDIIKVHFKNKADKPLSIHPQGIRYSKLSEGASYLDHTFPAEKMDD AVAPGREYTYEWSISEDSGPTHDDPPCLTHIYYSHENLIEDFNSGLIGPLLICKKGTLTEGGTQKTFDKQ IVLLFAVFDESKSWSQSSSLMYTVNGYVNGTMPDITVCAHDHISWHLLGMSSGPELFSIHFNGQVLEQNH HKVSAITLVSATSTTANMTVGPEGKWIISSLTPKHLQAGMQAYIDIKNCPKKTRNLKKITREQRRHMKRW EYFIAAEEVIWDYAPVIPANMDKKYRSQHLDNFSNQIGKHYKKVMYTQYEDESFTKHTVNPNMKEDGILG PIIRAQVRDTLKIVFKNMASRPYSIYPHGVTFSPYEDEVNSSFTSGRNNTMIRAVQPGETYTYKWNILEF DEPTENDAQCLTRPYYSDVDIMRDIASGLIGLLLICKSRSLDRRGIQRAADIEQQAVFAVFDENKSWYLE DNINKFCENPDEVKRDDPKFYESNIMSTINGYVPESITTLGFCFDDTVQWHFCSVGTQNEILTIHFTGHS FIYGKRHEDTLTLFPMRGESVTVTMDNVGTWMLTSMNSSPRSKKLRLKFRDVKCIPDDDEDSYEIFEPPE STVMATRKMHDRLEPEDEESDADYDYQNRLAAALGIRSFRNSSLNQEEEEFNLTALALENGTEFVSSNTD IIVGSNYSSPSNISKFTVNNLAEPQKAPSHQQATTAGSPLRHLIGKNSVLNSSTAEHSSPYSEDPIEDPL QPDVTGIRLLSLGAGEFRSQEHAKRKGPKVERDQAAKHRFSWMKLLAHKVGRHLSQDTGSPSGMRPWEDL PSQDTGSPSRMRPWEDPPSDLLLLKQSNSSKILVGRWHLASEKGSYEIIQDTDEDTAVNNWLISPQNASR AWGESTPLANKPGKQSGHPKFPRVRHKSLQVRQDGGKSRLKKSQFLIKTRKKKKEKHTHHAPLSPRTFHP LRSEAYNTFSERRLKHSLVLHKSNETSLPTDLNQTLPSMDFGWIASLPDHNQNSSNDTGQASCPPGLYQT VPPEEHYQTFPIQDPDQMHSTSDPSHRSSSPELSEMLEYDRSHKSFPTDISQMSPSSEHEVWQTVISPDL SQVTLSPELSQTNLSPDLSHTTLSPELIQRNLSPALGQMPISPDLSHTTLSPDLSHTTLSLDLSQTNLSP ELSQTNLSPALGQMPLSPDLSHTTISLDFSQTNLSPELSHMTLSPELSQTNLSPALGQMPISPDLSHTTL SLDFSQTNLSPELSQTNLSPALGQMPLSPDPSHTTLSLDLSQTNLSPELSQTNLSPDLSEMPLFADLSQI PLTPDLDQMTLSPDLGETDLSPNFGQMSLSPDLSQVTLSPDISDTTLLPDLSQISPPPDLDQIFYPSESS QSLLLQEFNESFPYPDLGQMPSPSSPTLNDTFLSKEFNPLVIVGLSKDGTDYIEIIPKEEVQSSEDDYAE IDYVPYDDPYKTDVRTNINSSRDPDNIAAWYLRSNNGNRRNYYIAAEEISWDYSEFVQRETDIEDSDDIP EDTTYKKVVFRKYLDSTFTKRDPRGEYEEHLGILGPIIRAEVDDVIQVRFKNLASRPYSLHAHGLSYEKS SEGKTYEDDSPEWFKEDNAVQPNSSYTYVWHATERSGPESPGSACRAWAYYSAVNPEKDIHSGLIGPLLI CQKGILHKDSNMPVDMREFVLLFMTFDEKKSWYYEKKSRSSWRLTSSEMKKSHEFHAINGMIYSLPGLKM YEQEWVRLHLLNIGGSQDIHVVHFHGQTLLENGNKQHQLGVWPLLPGSFKTLEMKASKPGWWLLNTEVGE NQRAGMQTPFLIMDRDCRMPMGLSTGIISDSQIKASEFLGYWEPRLARLNNGGSYNAWSVEKLAAEFASK PWIQVDMQKEVIITGIQTQGAKHYLKSCYTTEFYVAYSSNQINWQIFKGNSTRNVMYFNGNSDASTIKEN QFDPPIVARYIRISPTRAYNRPTLRLELQGCEVNGCSTPLGMENGKIENKQITASSFKKSWWGDYWEPFR ARLNAQGRVNAWQAKANNNKQWLEIDLLKIKKITAIITQGCKSLSSEMYVKSYTIHYSEQGVEWKPYRLK SSMVDKIFEGNTNTKGHVKNFFNPPIISRFIRVIPKTWNQSIALRLELFGCDIY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000130 |
| ORF Size | 6672 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000130.2, NP_000121.1 |
| RefSeq Size | 6914 bp |
| RefSeq ORF | 6675 bp |
| Locus ID | 2153 |
| UniProt ID | P12259 |
| Domains | F5_F8_type_C, Cu-oxidase |
| Protein Families | Druggable Genome |
| Protein Pathways | Complement and coagulation cascades |
| Gene Summary | This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008] |
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