Factor V (F5) (NM_000130) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FVL; PCCF; RPRGL1; THPH2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309876 representing NM_000130. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCCCAGGCTGCCCACGCCTCTGGGTCCTGGTGGTCTTGGGCACCAGCTGGGTAGGCTGGGGGAGC CAAGGGACAGAAGCGGCACAGCTAAGGCAGTTCTACGTGGCTGCTCAGGGCATCAGTTGGAGCTACCGA CCTGAGCCCACAAACTCAAGTTTGAATCTTTCTGTAACTTCCTTTAAGAAAATTGTCTACAGAGAGTAT GAACCATATTTTAAGAAAGAAAAACCACAATCTACCATTTCAGGACTTCTTGGGCCTACTTTATATGCT GAAGTCGGAGACATCATAAAAGTTCACTTTAAAAATAAGGCAGATAAGCCCTTGAGCATCCATCCTCAA GGAATTAGGTACAGTAAATTATCAGAAGGTGCTTCTTACCTTGACCACACATTCCCTGCGGAGAAGATG GACGACGCTGTGGCTCCAGGCCGAGAATACACCTATGAATGGAGTATCAGTGAGGACAGTGGACCCACC CATGATGACCCTCCATGCCTCACACACATCTATTACTCCCATGAAAATCTGATCGAGGATTTCAACTCG GGGCTGATTGGGCCCCTGCTTATCTGTAAAAAAGGGACCCTAACTGAGGGTGGGACACAGAAGACGTTT GACAAGCAAATCGTGCTACTATTTGCTGTGTTTGATGAAAGCAAGAGCTGGAGCCAGTCATCATCCCTA ATGTACACAGTCAATGGATATGTGAATGGGACAATGCCAGATATAACAGTTTGTGCCCATGACCACATC AGCTGGCATCTGCTGGGAATGAGCTCGGGGCCAGAATTATTCTCCATTCATTTCAACGGCCAGGTCCTG GAGCAGAACCATCATAAGGTCTCAGCCATCACCCTTGTCAGTGCTACATCCACTACCGCAAATATGACT GTGGGCCCAGAGGGAAAGTGGATCATATCTTCTCTCACCCCAAAACATTTGCAAGCTGGGATGCAGGCT TACATTGACATTAAAAACTGCCCAAAGAAAACCAGGAATCTTAAGAAAATAACTCGTGAGCAGAGGCGG CACATGAAGAGGTGGGAATACTTCATTGCTGCAGAGGAAGTCATTTGGGACTATGCACCTGTAATACCA GCGAATATGGACAAAAAATACAGGTCTCAGCATTTGGATAATTTCTCAAACCAAATTGGAAAACATTAT AAGAAAGTTATGTACACACAGTACGAAGATGAGTCCTTCACCAAACATACAGTGAATCCCAATATGAAA GAAGATGGGATTTTGGGTCCTATTATCAGAGCCCAGGTCAGAGACACACTCAAAATCGTGTTCAAAAAT ATGGCCAGCCGCCCCTATAGCATTTACCCTCATGGAGTGACCTTCTCGCCTTATGAAGATGAAGTCAAC TCTTCTTTCACCTCAGGCAGGAACAACACCATGATCAGAGCAGTTCAACCAGGGGAAACCTATACTTAT AAGTGGAACATCTTAGAGTTTGATGAACCCACAGAAAATGATGCCCAGTGCTTAACAAGACCATACTAC AGTGACGTGGACATCATGAGAGACATCGCCTCTGGGCTAATAGGACTACTTCTAATCTGTAAGAGCAGA TCCCTGGACAGGCGAGGAATACAGAGGGCAGCAGACATCGAACAGCAGGCTGTGTTTGCTGTGTTTGAT GAGAACAAAAGCTGGTACCTTGAGGACAACATCAACAAGTTTTGTGAAAATCCTGATGAGGTGAAACGT GATGACCCCAAGTTTTATGAATCAAACATCATGAGCACTATCAATGGCTATGTGCCTGAGAGCATAACT ACTCTTGGATTCTGCTTTGATGACACTGTCCAGTGGCACTTCTGTAGTGTGGGGACCCAGAATGAAATT TTGACCATCCACTTCACTGGGCACTCATTCATCTATGGAAAGAGGCATGAGGACACCTTGACCCTCTTC CCCATGCGTGGAGAATCTGTGACGGTCACAATGGATAATGTTGGAACTTGGATGTTAACTTCCATGAAT TCTAGTCCAAGAAGCAAAAAGCTGAGGCTGAAATTCAGGGATGTTAAATGTATCCCAGATGATGATGAA GACTCATATGAGATTTTTGAACCTCCAGAATCTACAGTCATGGCTACACGGAAAATGCATGATCGTTTA GAACCTGAAGATGAAGAGAGTGATGCTGACTATGATTACCAGAACAGACTGGCTGCAGCATTAGGAATC AGGTCATTCCGAAACTCATCATTGAATCAGGAAGAAGAAGAGTTCAATCTTACTGCCCTAGCTCTGGAG AATGGCACTGAATTCGTTTCTTCAAACACAGATATAATTGTTGGTTCAAATTATTCTTCCCCAAGTAAT ATTAGTAAGTTCACTGTCAATAACCTTGCAGAACCTCAGAAAGCCCCTTCTCACCAACAAGCCACCACA GCTGGTTCCCCACTGAGACACCTCATTGGCAAGAACTCAGTTCTCAATTCTTCCACAGCAGAGCATTCC AGCCCATATTCTGAAGACCCTATAGAGGATCCTCTACAGCCAGATGTCACAGGGATACGTCTACTTTCA CTTGGTGCTGGAGAATTCAAAAGTCAAGAACATGCTAAGCATAAGGGACCCAAGGTAGAAAGAGATCAA GCAGCAAAGCACAGGTTCTCCTGGATGAAATTACTAGCACATAAAGTTGGGAGACACCTAAGCCAAGAC ACTGGTTCTCCTTCCGGAATGAGGCCCTGGGAGGACCTTCCTAGCCAAGACACTGGTTCTCCTTCCAGA ATGAGGCCCTGGAAGGACCCTCCTAGTGATCTGTTACTCTTAAAACAAAGTAACTCATCTAAGATTTTG GTTGGGAGATGGCATTTGGCTTCTGAGAAAGGTAGCTATGAAATAATCCAAGATACTGATGAAGACACA GCTGTTAACAATTGGCTGATCAGCCCCCAGAATGCCTCACGTGCTTGGGGAGAAAGCACCCCTCTTGCC AACAAGCCTGGAAAGCAGAGTGGCCACCCAAAGTTTCCTAGAGTTAGACATAAATCTCTACAAGTAAGA CAGGATGGAGGAAAGAGTAGACTGAAGAAAAGCCAGTTTCTCATTAAGACACGAAAAAAGAAAAAAGAG AAGCACACACACCATGCTCCTTTATCTCCGAGGACCTTTCACCCTCTAAGAAGTGAAGCCTACAACACA TTTTCAGAAAGAAGACTTAAGCATTCGTTGGTGCTTCATAAATCCAATGAAACATCTCTTCCCACAGAC CTCAATCAGACATTGCCCTCTATGGATTTTGGCTGGATAGCCTCACTTCCTGACCATAATCAGAATTCC TCAAATGACACTGGTCAGGCAAGCTGTCCTCCAGGTCTTTATCAGACAGTGCCCCCAGAGGAACACTAT CAAACATTCCCCATTCAAGACCCTGATCAAATGCACTCTACTTCAGACCCCAGTCACAGATCCTCTTCT CCAGAGCTCAGTGAAATGCTTGAGTATGACCGAAGTCACAAGTCCTTCCCCACAGATATAAGTCAAATG TCCCCTTCCTCAGAACATGAAGTCTGGCAGACAGTCATCTCTCCAGACCTCAGCCAGGTGACCCTCTCT CCAGAACTCAGCCAGACAAACCTCTCTCCAGACCTCAGCCACACGACTCTCTCTCCAGAACTCATTCAG AGAAACCTTTCCCCAGCCCTCGGTCAGATGCCCATTTCTCCAGACCTCAGCCATACAACCCTTTCTCCA GACCTCAGCCATACAACCCTTTCTTTAGACCTCAGCCAGACAAACCTCTCTCCAGAACTCAGTCAGACA AACCTTTCTCCAGCCCTCGGTCAGATGCCCCTTTCTCCAGACCTCAGCCATACAACCCTTTCTCTAGAC TTCAGCCAGACAAACCTCTCTCCAGAACTCAGCCATATGACTCTCTCTCCAGAACTCAGTCAGACAAAC CTTTCCCCAGCCCTCGGTCAGATGCCCATTTCTCCAGACCTCAGCCATACAACCCTTTCTCTAGACTTC AGCCAGACAAACCTCTCTCCAGAACTCAGTCAAACAAACCTTTCCCCAGCCCTCGGTCAGATGCCCCTT TCTCCAGACCCCAGCCATACAACCCTTTCTCTAGACCTCAGCCAGACAAACCTCTCTCCAGAACTCAGT CAGACAAACCTTTCCCCAGACCTCAGTGAGATGCCCCTCTTTGCAGATCTCAGTCAAATTCCCCTTACC CCAGACCTCGACCAGATGACACTTTCTCCAGACCTTGGTGAGACAGATCTTTCCCCAAACTTTGGTCAG ATGTCCCTTTCCCCAGACCTCAGCCAGGTGACTCTCTCTCCAGACATCAGTGACACCACCCTTCTCCCG GATCTCAGCCAGATATCACCTCCTCCAGACCTTGATCAGATATTCTACCCTTCTGAATCTAGTCAGTCA TTGCTTCTTCAAGAATTTAATGAGTCTTTTCCTTATCCAGACCTTGGTCAGATGCCATCTCCTTCATCT CCTACTCTCAATGATACTTTTCTATCAAAGGAATTTAATCCACTGGTTATAGTGGGCCTCAGTAAAGAT GGTACAGATTACATTGAGATCATTCCAAAGGAAGAGGTCCAGAGCAGTGAAGATGACTATGCTGAAATT GATTATGTGCCCTATGATGACCCCTACAAAACTGATGTTAGGACAAACATCAACTCCTCCAGAGATCCT GACAACATTGCAGCATGGTACCTCCGCAGCAACAATGGAAACAGAAGAAATTATTACATTGCTGCTGAA GAAATATCCTGGGATTATTCAGAATTTGTACAAAGGGAAACAGATATTGAAGACTCTGATGATATTCCA GAAGATACCACATATAAGAAAGTAGTTTTTCGAAAGTACCTCGACAGCACTTTTACCAAACGTGATCCT CGAGGGGAGTATGAAGAGCATCTCGGAATTCTTGGTCCTATTATCAGAGCTGAAGTGGATGATGTTATC CAAGTTCGTTTTAAAAATTTAGCATCCAGACCGTATTCTCTACATGCCCATGGACTTTCCTATGAAAAA TCATCAGAGGGAAAGACTTATGAAGATGACTCTCCTGAATGGTTTAAGGAAGATAATGCTGTTCAGCCA AATAGCAGTTATACCTACGTATGGCATGCCACTGAGCGATCAGGGCCAGAAAGTCCTGGCTCTGCCTGT CGGGCTTGGGCCTACTACTCAGCTGTGAACCCAGAAAAAGATATTCACTCAGGCTTGATAGGTCCCCTC CTAATCTGCCAAAAAGGAATACTACATAAGGACAGCAACATGCCTATGGACATGAGAGAATTTGTCTTA CTATTTATGACCTTTGATGAAAAGAAGAGCTGGTACTATGAAAAGAAGTCCCGAAGTTCTTGGAGACTC ACATCCTCAGAAATGAAAAAATCCCATGAGTTTCACGCCATTAATGGGATGATCTACAGCTTGCCTGGC CTGAAAATGTATGAGCAAGAGTGGGTGAGGTTACACCTGCTGAACATAGGCGGCTCCCAAGACATTCAC GTGGTTCACTTTCACGGCCAGACCTTGCTGGAAAATGGCAATAAACAGCACCAGTTAGGGGTCTGGCCC CTTCTGCCTGGTTCATTTAAAACTCTTGAAATGAAGGCATCAAAACCTGGCTGGTGGCTCCTAAACACA GAGGTTGGAGAAAACCAGAGAGCAGGGATGCAAACGCCATTTCTTATCATGGACAGAGACTGTAGGATG CCAATGGGACTAAGCACTGGTATCATATCTGATTCACAGATCAAGGCTTCAGAGTTTCTGGGTTACTGG GAGCCCAGATTAGCAAGATTAAACAATGGTGGATCTTATAATGCTTGGAGTGTAGAAAAACTTGCAGCA GAATTTGCCTCTAAACCTTGGATCCAGGTGGACATGCAAAAGGAAGTCATAATCACAGGGATCCAGACC CAAGGTGCCAAACACTACCTGAAGTCCTGCTATACCACAGAGTTCTATGTAGCTTACAGTTCCAACCAG ATCAACTGGCAGATCTTCAAAGGGAACAGCACAAGGAATGTGATGTATTTTAATGGCAATTCAGATGCC TCTACAATAAAAGAGAATCAGTTTGACCCACCTATTGTGGCTAGATATATTAGGATCTCTCCAACTCGA GCCTATAACAGACCTACCCTTCGATTGGAACTGCAAGGTTGTGAGGTAAATGGATGTTCCACACCCCTG GGTATGGAAAATGGAAAGATAGAAAACAAGCAAATCACAGCTTCTTCGTTTAAGAAATCTTGGTGGGGA GATTACTGGGAACCCTTCCGTGCCCGTCTGAATGCCCAGGGACGTGTGAATGCCTGGCAAGCCAAGGCA AACAACAATAAGCAGTGGCTAGAAATTGATCTACTCAAGATCAAGAAGATAACGGCAATTATAACACAG GGCTGCAAGTCTCTGTCCTCTGAAATGTATGTAAAGAGCTATACCATCCACTACAGTGAGCAGGGAGTG GAATGGAAACCATACAGGCTGAAATCCTCCATGGTGGACAAGATTTTTGAAGGAAATACTAATACCAAA GGACATGTGAAGAACTTTTTCAACCCCCCAATCATTTCCAGGTTTATCCGTGTCATTCCTAAAACATGG AATCAAAGTATTGCACTTCGCCTGGAACTCTTTGGCTGTGATATTTACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_000130
Insert Size	6675 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_000130.4
RefSeq Size	9179 bp
RefSeq ORF	6675 bp
Locus ID	2153
UniProt ID	P12259
Domains	F5_F8_type_C, Cu-oxidase
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades
MW	251.7 kDa
Gene Summary	This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.