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NuMA (NUMA1) (NM_006185) Human Tagged ORF Clone
CAT＃: RG215124
- TrueORF®
NUMA1 (tGFP-tagged) - Human nuclear mitotic apparatus protein 1 (NUMA1)
ORF Plasmid: DDK
"NM_006185" in other vectors (2)
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CNY 12,640.00
货期*
13周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NMP-22; NUMA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215124 representing NM_006185
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACACTCCACGCCACCCGGGGGGCTGCACTCCTCTCTTGGGTGAACAGTCTACACGTGGCTGACCCTG TGGAGGCTGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATCATTGACAGAATCCATGGCACTGA AGAGGGACAGCAAATCTTGAAGCAGCCGGTGTCAGAGAGACTGGACTTTGTGTGCAGTTTTCTGCAGAAA AATCGAAAACATCCCTCTTCCCCAGAATGCCTGGTATCTGCACAGAAGGTGCTAGAGGGATCAGAGCTGG AACTGGCGAAGATGACCATGCTGCTCTTATACCACTCTACCATGAGCTCCAAAAGTCCCAGGGACTGGGA ACAGTTTGAATATAAAATTCAGGCTGAGTTGGCTGTCATTCTTAAATTTGTGCTGGACCATGAGGACGGG CTAAACCTTAATGAGGACCTAGAGAACTTCCTACAGAAAGCTCCTGTGCCTTCTACCTGTTCTAGCACAT TCCCTGAAGAGCTCTCCCCACCTAGCCACCAGGCCAAGAGGGAGATTCGCTTCCTAGAGCTACAGAAGGT TGCCTCCTCTTCCAGTGGGAACAACTTTCTCTCAGGTTCTCCAGCTTCTCCCATGGGTGATATCCTGCAG ACCCCACAGTTCCAGATGAGACGGCTGAAGAAGCAGCTTGCTGATGAGAGAAGTAATAGGGATGAGCTGG AGCTGGAGCTAGCTGAGAACCGCAAGCTCCTCACCGAGAAGGATGCACAGATAGCCATGATGCAGCAGCG CATTGACCGCCTAGCCCTGCTGAATGAGAAGCAGGCGGCCAGCCCACTGGAGCCCAAGGAGCTTGAGGAG CTGCGTGACAAGAATGAGAGCCTTACCATGCGGCTGCATGAAACCCTGAAGCAGTGCCAGGACCTGAAGA CAGAGAAGAGCCAGATGGATCGCAAAATCAACCAGCTTTCGGAGGAGAATGGAGACCTTTCCTTTAAGCT GCGGGAGTTTGCCAGTCATCTGCAGCAGCTACAGGATGCCCTCAATGAGCTGACGGAGGAGCACAGCAAG GCCACTCAGGAGTGGCTAGAGAAGCAGGCCCAGCTGGAGAAGGAGCTCAGCGCAGCCCTGCAGGACAAGA AATGCCTTGAAGAGAAGAACGAAATCCTTCAGGGAAAACTTTCACAGCTGGAAGAACACTTGTCCCAGCT GCAGGATAACCCACCCCAGGAGAAGGGCGAGGTGCTGGGTGATGTCTTGCAGCTGGAAACCTTGAAGCAA GAGGCAGCCACTCTTGCTGCAAACAACACACAGCTCCAAGCCAGGGTAGAGATGCTGGAGACTGAGCGAG GCCAGCAGGAAGCCAAGCTGCTTGCTGAGCGGGGCCACTTCGAAGAAGAAAAGCAGCAGCTGTCTAGCCT GATCACTGACCTGCAGAGCTCCATCTCCAACCTCAGCCAGGCCAAGGAAGAGCTGGAGCAGGCCTCCCAG GCTCATGGGGCCCGGTTGACTGCCCAGGTGGCCTCTCTGACCTCTGAGCTCACCACACTCAATGCCACCA TCCAGCAACAGGATCAAGAACTGGCTGGCCTGAAGCAGCAGGCCAAAGAGAAGCAGGCCCAGCTAGCACA GACCCTCCAACAGCAAGAACAGGCCTCCCAGGGCCTCCGCCACCAGGTGGAGCAGCTAAGCAGTAGCCTG AAGCAGAAGGAGCAGCAGTTGAAGGAGGTAGCGGAGAAGCAGGAGGCAACTAGGCAGGACCATGCCCAGC AACTGGCCACTGCTGCAGAGGAGCGAGAGGCCTCCTTAAGGGAGCGGGATGCGGCTCTCAAGCAGCTGGA GGCACTGGAGAAGGAGAAGGCTGCCAAGCTGGAGATTCTGCAGCAGCAACTTCAGGTGGCTAATGAAGCC CGGGACAGTGCCCAGACCTCAGTGACACAGGCCCAGCGGGAGAAGGCAGAGCTGAGCCGGAAGGTGGAGG AACTCCAGGCCTGTGTTGAGACAGCCCGCCAGGAACAGCATGAGGCCCAGGCCCAGGTTGCAGAGCTAGA GTTGCAGCTGCGGTCTGAGCAGCAAAAAGCAACTGAGAAAGAAAGGGTGGCCCAGGAGAAGGACCAGCTC CAGGAGCAGCTCCAGGCCCTCAAAGAGTCCTTGAAGGTCACCAAGGGCAGCCTTGAAGAGGAGAAGCGCA GGGCTGCAGATGCCCTGGAAGAGCAGCAGCGTTGTATCTCTGAGCTGAAGGCAGAGACCCGAAGCCTGGT GGAGCAGCATAAGCGGGAACGAAAGGAGCTGGAAGAAGAGAGGGCTGGGCGCAAGGGGCTGGAGGCTCGA TTACAGCAGCTTGGGGAGGCCCATCAGGCTGAGACTGAAGTCCTGCGGCGGGAGCTGGCAGAGGCCATGG CTGCCCAGCACACAGCTGAGAGTGAGTGTGAGCAGCTCGTCAAAGAAGTAGCTGCCTGGCGTGAGCGGTA TGAGGATAGCCAGCAAGAGGAGGCACAGTATGGCGCCATGTTCCAGGAACAGCTGATGACTTTGAAGGAG GAATGTGAGAAGGCCCGCCAGGAGCTGCAGGAGGCAAAGGAGAAGGTGGCAGGCATAGAATCCCACAGCG AGCTCCAGATAAGCCGGCAGCAGAACGAACTAGCTGAGCTCCATGCCAACCTGGCCAGAGCACTCCAGCA GGTCCAAGAGAAGGAAGTCAGGGCCCAGAAGCTTGCAGATGACCTCTCCACTCTGCAGGAAAAGATGGCT GCCACCAGCAAAGAGGTGGCCCGCTTGGAGACCTTGGTGCGCAAGGCAGGTGAGCAGCAGGAAACAGCCT CCCGGGAGTTAGTCAAGGAGCCTGCGAGGGCAGGAGACAGACAGCCCGAGTGGCTGGAAGAGCAACAGGG ACGCCAGTTCTGCAGCACACAGGCAGCGCTGCAGGCTATGGAGCGGGAGGCAGAGCAGATGGGCAATGAG CTGGAACGGCTGCGGGCCGCGCTGATGGAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGGCAGCAGGAAA GGGAGGTGGCGCGGCTGACCCAGGAGCGGGGCCGTGCCCAGGCTGACCTTGCCCTGGAGAAGGCGGCCAG AGCAGAGCTTGAGATGCGGCTGCAGAACGCCCTCAACGAGCAGCGTGTGGAGTTCGCTACCCTGCAAGAG GCACTGGCTCATGCCCTGACGGAAAAGGAAGGCAAGGACCAGGAGTTGGCCAAGCTTCGTGGTCTGGAGG CAGCCCAGATAAAAGAGCTGGAGGAACTTCGGCAAACCGTGAAGCAACTGAAGGAACAGCTGGCTAAGAA AGAAAAGGAGCACGCATCTGGCTCAGGAGCCCAATCTGAGGCTGCTGGCAGGACAGAGCCAACAGGCCCC AAGCTGGAGGCACTGCGGGCAGAGGTGAGCAAGCTGGAACAGCAATGCCAGAAGCAGCAGGAGCAGGCTG ACAGCCTGGAACGCAGCCTCGAGGCTGAGCGGGCCTCCCGGGCTGAGCGGGACAGTGCTCTGGAGACTCT GCAGGGCCAGTTAGAGGAGAAGGCCCAGGAGCTAGGGCACAGTCAGAGTGCCTTAGCCTCGGCCCAACGG GAGTTGGCTGCCTTCCGCACCAAGGTACAAGACCACAGCAAGGCTGAAGATGAGTGGAAGGCCCAGGTGG CCCGGGGCCGGCAAGAGGCTGAGAGGAAAAATAGCCTCATCAGCAGCTTGGAGGAGGAGGTGTCCATCCT GAATCGCCAGGTCCTGGAGAAGGAGGGGGAGAGCAAGGAGTTGAAGCGGCTGGTGATGGCCGAGTCAGAG AAGAGCCAGAAGCTGGAGGAGAGGCTGCGCCTGCTGCAGGCAGAGACAGCCAGCAACAGTGCCAGAGCTG CAGAACGCAGCTCTGCTCTGCGGGAGGAGGTGCAGAGCCTCCGGGAGGAGGCTGAGAAACAGCGGGTGGC TTCAGAGAACCTGCGGCAGGAGCTGACCTCACAGGCTGAGCGTGCGGAGGAGCTGGGCCAAGAATTGAAG GCGTGGCAGGAGAAGTTCTTCCAGAAAGAGCAGGCCCTCTCCACCCTGCAGCTCGAGCACACCAGCACAC AGGCCCTGGTGAGTGAGCTGCTGCCAGCTAAGCACCTCTGCCAGCAGCTGCAGGCCGAGCAGGCCGCTGC CGAGAAACGCCACCGTGAGGAGCTGGAGCAGAGCAAGCAGGCCGCTGGGGGACTGCGGGCAGAGCTGCTG CGGGCCCAGCGGGAGCTTGGGGAGCTGATTCCTCTGCGGCAGAAGGTGGCAGAGCAGGAGCGAACAGCTC AGCAGCTGCGGGCAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCGCATGGCCTGCT GGCAGAGGAGAACCGGGGGCTGGGTGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAAGTGGAGTTGGAC CAGGCCCGGGAGAAGTATGTCCAAGAGTTGGCAGCCGTACGTGCTGATGCTGAGACCCGTCTGGCTGAGG TGCAGCGAGAAGCACAGAGCACTGCCCGGGAGCTGGAGGTGATGACTGCCAAGTATGAGGGTGCCAAGGT CAAGGTCCTGGAGGAGAGGCAGCGGTTCCAGGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAGCAGCTA GAGGTATTTCAGAGAGAGCAAACTAAGCAGGTGGAAGAACTGAGTAAGAAACTGGCTGACTCTGACCAAG CCAGCAAGGTGCAGCAGCAGAAGCTGAAGGCTGTCCAGGCTCAGGGAGGCGAGAGCCAGCAGGAGGCCCA GCGCCTCCAGGCCCAGCTGAATGAACTGCAAGCCCAGTTGAGCCAGAAGGAGCAGGCAGCTGAGCACTAT AAGCTGCAGATGGAGAAAGCCAAAACACATTATGATGCCAAGAAGCAGCAGAACCAAGAGCTGCAGGAGC AGCTGCGGAGCCTGGAGCAGCTGCAGAAGGAAAACAAAGAGCTGCGAGCTGAAGCTGAACGGCTGGGCCA TGAGCTACAGCAGGCTGGGCTGAAGACCAAGGAGGCTGAACAGACCTGCCGCCACCTTACTGCCCAGGTG CGCAGCCTGGAGGCACAGGTTGCCCATGCAGACCAGCAGCTTCGAGACCTGGGCAAATTCCAGGTGGCAA CTGATGCTTTAAAGAGCCGTGAGCCCCAGGCTAAGCCCCAGCTGGACTTGAGTATTGACAGCCTGGATCT GAGCTGCGAGGAGGGGACCCCACTCAGTATCACCAGCAAGCTGCCTCGTACCCAGCCAGACGGCACCAGC GTCCCTGGAGAACCAGCCTCACCTATCTCCCAGCGCCTGCCCCCCAAGGTAGAATCCCTGGAGAGTCTCT ACTTCACTCCCATCCCTGCTCGGAGTCAGGCCCCCCTGGAGAGCAGCCTGGACTCCCTGGGAGACGTCTT CCTGGACTCGGGTCGTAAGACCCGCTCCGCTCGTCGGCGCACCACGCAGATCATCAACATCACCATGACC AAGAAGCTAGATGTGGAAGAGCCAGACAGCGCCAACTCATCGTTCTACAGCACGCGGTCTGCTCCTGCTT CCCAGGCTAGCCTGCGAGCCACCTCCTCTACTCAGTCTCTAGCTCGCCTGGGTTCTCCCGATTATGGCAA CTCAGCCCTGCTCAGCTTGCCTGGCTACCGCCCCACCACTCGCAGTTCTGCTCGTCGTTCCCAGGCCGGG GTGTCCAGTGGGGCCCCTCCAGGAAGGAACAGCTTCTACATGGGCACTTGCCAGGATGAGCCTGAGCAGC TGGATGACTGGAACCGCATTGCAGAGCTGCAGCAGCGCAATCGAGTGTGCCCCCCACATCTGAAGACCTG CTATCCCCTGGAGTCCAGGCCTTCCCTGAGCCTGGGCACCATCACAGATGAGGAGATGAAAACTGGAGAC CCCCAAGAGACCCTGCGCCGAGCCAGCATGCAGCCAATCCAGATAGCCGAGGGCACTGGCATCACCACCC GGCAGCAGCGCAAACGGGTCTCCCTAGAGCCCCACCAGGGCCCTGGAACTCCTGAGTCTAAGAAGGCCAC CAGCTGTTTCCCACGCCCCATGACTCCCCGAGACCGACATGAAGGGCGCAAACAGAGCACTACTGAGGCC CAGAAGAAAGCAGCTCCAGCTTCTACTAAACAGGCTGACCGGCGCCAGTCGATGGCCTTCAGCATCCTCA ACACACCCAAGAAGCTAGGGAACAGCCTTCTGCGGCGGGGAGCCTCAAAGAAGGCCCTGTCCAAGGCTTC CCCCAACACTCGCAGTGGAACCCGCCGTTCTCCGCGCATTGCCACCACCACAGCCAGCGCCGCCACTGCT GCCGCCATTGGTGCCACCCCTCGAGCCAAGGGCAAGGCAAAGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215124 representing NM_006185
Red=Cloning site Green=Tags(s) MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQK NRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDG LNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQ TPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEE LRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSK ATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQ EAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQ AHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSL KQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEA RDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQL QEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEAR LQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKE ECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMA ATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNE LERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQE ALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGP KLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQR ELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESE KSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELK AWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELL RAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELD QAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEQL EVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHY KLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQV RSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTS VPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMT KKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAG VSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGD PQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEA QKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATA AAIGATPRAKGKAKH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006185 |
| ORF Size | 6345 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006185.4 |
| RefSeq Size | 7182 bp |
| RefSeq ORF | 6348 bp |
| Locus ID | 4926 |
| UniProt ID | Q14980 |
| Domains | M |
| Gene Summary | This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013] |
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