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PPP1R3A (NM_002711) Human Tagged ORF Clone
CAT＃: RG215004
- TrueORF®
PPP1R3A (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A)
ORF Plasmid: DDK
"NM_002711" in other vectors (6)
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CNY 9,416.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GM; PP1G; PPP1R3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215004 representing NM_002711
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCTTCTGAAGTACCTAGTCAGATTAGCAAAGATAATTTTTTAGAAGTTCCTAATTTATCTGACT CTCTTTGTGAAGATGAAGAAGTTACTTTCCAACCTGGTTTCTCCCCTCAACCAAGTAGACGAGGTTCTGA TTCTTCTGAAGACATATACCTGGATACCCCATCTTCAGGTACTAGAAGAGTTTCATTTGCTGATTCCTTT GGATTCAATCTTGTGTCTGTTAAAGAATTTGATTGCTGGGAATTACCGAGTGCTTCAACCACTTTTGACT TAGGGACGGACATTTTCCACACAGAAGAATATGTTTTAGCCCCACTGTTTGACTTGCCTTCTTCAAAAGA AGATCTTATGCAACAACTCCAAATACAGAAAGCAATACTGGAGTCAACTGAGTCTCTTCTTGGGTCTACA AGTATCAAGGGTATTATTCGAGTTTTGAATGTTTCTTTTGAGAAGTTAGTATATGTAAGAATGTCTTTAG ATGACTGGCAGACACATTATGACATTTTAGCAGAATATGTTCCTAATTCATGTGATGGTGAAACTGACCA GTTCTCCTTTAAGATTGTATTGGTTCCTCCTTATCAAAAAGATGGCAGTAAAGTTGAGTTTTGTATACGT TATGAAACTTCTGTTGGTACATTTTGGTCAAATAATAATGGCACAAATTATACATTCATTTGTCAAAAGA AAGAACAAGAGCCGGAGCCTGTAAAACCATGGAAAGAAGTTCCTAACAGACAAATAAAAGGCTGCTTAAA GGTAAAATCAAGTAAAGAAGAATCATCAGTAACATCAGAAGAAAATAACTTTGAGAATCCAAAGAATACA GATACCTATATCCCAACAATCATTTGTTCTCATGAGGACAAGGAAGATTTGGAAGCCAGTAATCGAAATG TAAAAGATGTAAACAGGGAACATGATGAACATAATGAAAAAGAATTAGAGTTGATGATAAATCAACACTT AATAAGAACCAGAAGTACTGCTTCCAGAGATGAAAGGAATACATTTTCAACAGATCCAGTCAATTTTCCA AATAAAGCAGAGGGGTTAGAGAAGAAGCAAATCCATGGTGAAATATGTACTGACTTGTTCCAAAGGTCTC TGTCTCCAAGTTCATCAGCAGAAAGCTCCGTAAAGGGAGATTTTTACTGCAATGAAAAATATTCCTCAGG AGATGACTGTACACATCAACCTTCAGAGGAAACTACTTCAAATATGGGAGAAATCAAGCCATCATTGGGA GATACTAGTAGTGATGAACTAGTGCAATTACATACTGGCAGCAAAGAAGTCCTGGATGATAATGCTAATC CAGCCCATGGCAATGGCACAATGCAAATACCTTGCCCCTCTTCAGATCAACTAATGGCAGGAAACCTTAA TAAAAAACATGAAGGAGGAGCTAAAAAAATTGAAGTAAAAGATTTGGGATGTTTACGAAGAGATTTCCAT TCAGATACGTCGGCATGTCTCAAAGAATCAACAGAAGAAGGATCTTCTAAGGAAGATTATTATGGCAATG GTAAGGATGATGAAGAACAAAGAATATATTTAGGTGTTAATGAAAAACAAAGAAAAAATTTCCAAACAAT CTTACATGACCAAGAAAGGAAGATGGGTAACCCTAAAATAAGTGTGGCAGGGATTGGAGCTAGTAACAGA GACCTGGCTACTCTGCTGAGCGAACATACCGCAATCCCCACCCGGGCAATCACAGCAGATGTGTCTCATT CACCAAGGACAAATTTAAGTTGGGAAGAAGCTGTGTTAACCCCAGAGCATCATCATTTGACTAGTGAAGG CAGCGCTTTAGGAGGGATAACTGGTCAAGTTTGTTCATCAAGAACTGGAAATGTTTTGAGGAATGATTAT CTTTTCCAAGTTGAAGAAAAATCAGGTGGGATTAATTCTGAAGATCAGGATAATAGCCCACAGCATAAAC AAAGTTGGAATGTTCTGGAAAGTCAGGGAAAATCAAGAGAGAATAAGACAAACATAACAGAGCATATCAA AGGACAAACAGATTGTGAAGACGTGTGGGGAAAAAGAGATAATACGAGGAGTTTGAAAGCTACTACAGAA GAATTGTTTACCTGCCAAGAAACAGTGTGCTGTGAACTGTCTTCTCTAGCTGATCATGGCATTACTGAGA AAGCAGAAGCTGGTACAGCCTATATAATTAAGACAACATCAGAAAGTACTCCAGAAAGCATGTCTGCTAG AGAAAAAGCAATAATTGCTAAGCTACCTCAAGAGACAGCACGAAGTGACAGGCCCATCGAGGTAAAGGAA ACAGCGTTTGATCCACATGAAGGGAGAAATGATGATTCACATTATACCCTTTGTCAACGAGATACAGTAG GTGTAATCTATGACAATGATTTTGAAAAGGAATCACGTTTAGGTATTTGTAATGTACGTGTAGATGAAAT GGAGAAGGAAGAAACCATGTCTATGTACAATCCTAGGAAGACACATGACAGGGAGAAATGTGGCACTGGA AATATAACATCTGTGGAAGAATCCTCATGGGTCATTACAGAATATCAAAAAGCAACTTCAAAACTGGATT TACAGTTGGGAATGTTACCAACAGACAAAACTGTATTTTCAGAAAACAGAGATCATAGGCAGGTTCAAGA ATTATCAAAGAAAACAGACTCGGATGCCATTGTGCATTCTGCTTTTAACTCAGACACTAATAGAGCTCCT CAGAATAGCTCTCCTTTTTCCAAACATCATACTGAAATTTCAGTGTCAACTAATGAGCAGGCAATTGCTG TAGAGAATGCAGTTACTACCATGGCTAGCCAACCTATTTCTACGAAATCAGAAAATATTTGTAATTCAAC AAGAGAAATCCAGGGTATTGAGAAGCACCCTTATCCTGAGTCTAAACCTGAAGAAGTTTCCAGAAGTTCA GGAATAGTGACATCAGGTAGTAGAAAAGAAAGATGCATAGGCCAGATTTTCCAAACAGAAGAGTATAGTG TGGAAAAATCTCTAGGGCCAATGATTTTAATCAACAAACCTCTTGAGAATATGGAAGAAGCAAGGCATGA AAATGAAGGATTAGTAAGCTCTGGGCAATCACTATACACTTCAGGTGAAAAGGAATCTGACAGCTCTGCT TCTACTAGTCTTCCTGTTGAGGAAAGTCAAGCTCAAGGCAACGAATCTTTGTTTTCAAAATATACCAACT CTAAAATACCTTATTTCCTTTTGTTTCTGATATTTCTTATAACTGTCTACCATTATGACTTAATGATTGG CTTGACATTCTACGTTTTGTCATTGTCCTGGCTATCCTGGGAAGAGGGTAGACAAAAAGAGTCTGTCAAA AAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215004 representing NM_002711
Red=Cloning site Green=Tags(s) MEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSGTRRVSFADSF GFNLVSVKEFDCWELPSASTTFDLGTDIFHTEEYVLAPLFDLPSSKEDLMQQLQIQKAILESTESLLGST SIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKIVLVPPYQKDGSKVEFCIR YETSVGTFWSNNNGTNYTFICQKKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNT DTYIPTIICSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFP NKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLG DTSSDELVQLHTGSKEVLDDNANPAHGNGTMQIPCPSSDQLMAGNLNKKHEGGAKKIEVKDLGCLRRDFH SDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNR DLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDY LFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTE ELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKE TAFDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTG NITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDHRQVQELSKKTDSDAIVHSAFNSDTNRAP QNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSS GIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSA STSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVK KK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002711 |
| ORF Size | 3366 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002711.2, NP_002702.1 |
| RefSeq Size | 6779 bp |
| RefSeq ORF | 3369 bp |
| Locus ID | 5506 |
| UniProt ID | Q16821 |
| Protein Families | Druggable Genome, Phosphatase, Transmembrane |
| Protein Pathways | Insulin signaling pathway |
| Gene Summary | The glycogen-associated form of protein phosphatase-1 (PP1) derived from skeletal muscle is a heterodimer composed of a 37-kD catalytic subunit and a 124-kD targeting and regulatory subunit. This gene encodes the regulatory subunit which binds to muscle glycogen with high affinity, thereby enhancing dephosphorylation of glycogen-bound substrates for PP1 such as glycogen synthase and glycogen phosphorylase kinase. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215004 | PPP1R3A (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A) |
CNY 7,816.00 |
|
| RC215004L1 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A), Myc-DDK-tagged |
CNY 10,216.00 |
|
| RC215004L2 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A), mGFP tagged |
CNY 7,600.00 |
|
| RC215004L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A), Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC215004L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A), mGFP tagged |
CNY 7,600.00 |
|
| SC125330 | PPP1R3A (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3A (PPP1R3A) |
CNY 10,450.00 |
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