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RAP1GAP2 (NM_001100398) Human Tagged ORF Clone
CAT＃: RG214554
- TrueORF®
RAP1GAP2 (tGFP-tagged) - Human RAP1 GTPase activating protein 2 (RAP1GAP2), transcript variant 2
ORF Plasmid: DDK
"NM_001100398" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GARNL4; RAP1GA3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214554 representing NM_001100398
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTGGCCGGAAGCGCAGTGTCTCCTTTGGGGGCTTCGGATGGATCGACAAGACCATGCTGGCAAGTC TGAAGGTCAAGAAGCAGGAGCTGGCCAACAGCTCGGATGCGACCCTCCCAGACCGGCCGCTCTCCCCTCC TCTCACGGCACCTCCCACCATGAAGTCGTCGGAGTTCTTTGAGATGCTGGAGAAAATGCAGGACGACTAT ATCCCATACCCCAGCATCGACGAGGTTGTGGAGAAGGGAGGCCCGTACCCTCAGGTCATCCTGCCACAGT TTGGGGGCTATTGGATCGAGGACCCGGAGAACGTGGGCACCCCAACATCGCTGGGGAGCAGCATCTGTGA GGAGGAGGAAGAGGACAACCTCAGCCCCAACACATTTGGCTACAAGCTCGAGTGCAAGGGTGAAGCCAGG GCCTACCGGAGGCACTTCCTGGGGAAGGATCATCTAAACTTTTACTGTACCGGCAGCAGCCTGGGGAACT TGATCCTGTCCGTCAAGTGCGAGGAAGCAGAGGGGATCGAGTACCTCCGGGTCATCCTTAGGTCCAAACT GAAGACGGTACATGAGCGGATCCCCTTGGCTGGACTGAGCAAGCTTCCCAGTGTCCCTCAGATTGCAAAG GCTTTCTGTGATGATGCAGTGGGACTGAGATTCAATCCTGTCCTGTACCCCAAGGCCTCCCAAATGATTG TGTCCTATGATGAGCATGAAGTCAACAACACATTCAAATTCGGAGTCATTTATCAAAAAGCCAGGCAGAC CCTGGAGGAGGAGCTATTTGGGAACAATGAGGAGAGCCCAGCTTTTAAGGAGTTCTTGGACCTGCTGGGG GACACGATCACACTGCAGGATTTCAAAGGTTTCCGAGGAGGCCTGGACGTGACCCACGGACAGACAGGGG TGGAATCAGTGTACACAACATTCCGGGACAGGGAGATCATGTTTCACGTTTCCACAAAGCTGCCATTTAC CGACGGAGACGCCCAGCAGCTCCAGAGAAAGAGACACATTGGAAATGACATCGTGGCCATCATCTTCCAA GAGGAAAACACGCCGTTTGTCCCAGACATGATAGCCTCCAATTTCTTACATGCCTACATCGTCGTGCAGG TCGAGACCCCAGGCACAGAGACCCCATCCTACAAGGTCTCTGTCACTGCGCGGGAAGATGTGCCCACCTT TGGTCCACCTCTGCCCAGTCCCCCCGTTTTCCAGAAGGGCCCGGAATTCAGGGAGTTTCTGCTCACCAAG CTCACCAATGCCGAGAACGCCTGCTGCAAGTCGGACAAGTTTGCAAAGCTGGAGGACCGGACCAGGGCTG CCCTCCTGGACAACCTTCACGATGAGCTCCACGCCCACACACAGGCCATGCTGGGACTGGGCCCAGAGGA GGACAAGTTTGAGAATGGAGGCCACGGGGGGTTCCTGGAGTCTTTTAAGAGGGCCATCCGCGTACGCAGC CACTCCATGGAGACCATGGTGGGCGGCCAGAAGAAGTCGCACAGTGGGGGCATCCCTGGCAGCCTCAGCG GGGGCATCTCCCACAACAGCATGGAGGTCACCAAGACCACCTTCTCGCCTCCAGTGGTGGCGGCAACGGT GAAGAACCAGTCACGGAGTCCCATCAAGCGACGCTCGGGGCTCTTCCCCCGCCTGCACACGGGCTCAGAA GGCCAGGGCGACAGCCGGGCACGATGTGACAGCACATCCAGCACACCCAAGACCCCAGATGGTGGACACT CCTCTCAGGAGATAAAGTCTGAGACCTCATCCAATCCCAGCTCTCCGGAAATCTGCCCCAACAAGGAGAA GCCCTTCATGAAGTTGAAGGAAAACGGCCGTGCCATCTCCCGCTCCTCCTCCAGCACCAGCAGCGTCAGC AGCACTGCAGGGGAGGGCGAGGCCATGGAGGAGGGCGACAGTGGGGGCAGCCAGCCGTCCACGACCTCAC CCTTCAAGCAGGAGGTGTTTGTCTACAGCCCGTCCCCGAGCAGCGAGAGCCCCAGCCTGGGGGCAGCTGC CACCCCGATCATCATGAGCCGGAGTCCCACAGATGCCAAAAGCAGAAACTCCCCGAGATCGAACCTGAAA TTCCGCTTTGACAAGCTCAGCCATGCCAGCTCTGGTGCGGGTCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214554 representing NM_001100398
Red=Cloning site Green=Tags(s) MFGRKRSVSFGGFGWIDKTMLASLKVKKQELANSSDATLPDRPLSPPLTAPPTMKSSEFFEMLEKMQDDY IPYPSIDEVVEKGGPYPQVILPQFGGYWIEDPENVGTPTSLGSSICEEEEEDNLSPNTFGYKLECKGEAR AYRRHFLGKDHLNFYCTGSSLGNLILSVKCEEAEGIEYLRVILRSKLKTVHERIPLAGLSKLPSVPQIAK AFCDDAVGLRFNPVLYPKASQMIVSYDEHEVNNTFKFGVIYQKARQTLEEELFGNNEESPAFKEFLDLLG DTITLQDFKGFRGGLDVTHGQTGVESVYTTFRDREIMFHVSTKLPFTDGDAQQLQRKRHIGNDIVAIIFQ EENTPFVPDMIASNFLHAYIVVQVETPGTETPSYKVSVTAREDVPTFGPPLPSPPVFQKGPEFREFLLTK LTNAENACCKSDKFAKLEDRTRAALLDNLHDELHAHTQAMLGLGPEEDKFENGGHGGFLESFKRAIRVRS HSMETMVGGQKKSHSGGIPGSLSGGISHNSMEVTKTTFSPPVVAATVKNQSRSPIKRRSGLFPRLHTGSE GQGDSRARCDSTSSTPKTPDGGHSSQEIKSETSSNPSSPEICPNKEKPFMKLKENGRAISRSSSSTSSVS STAGEGEAMEEGDSGGSQPSTTSPFKQEVFVYSPSPSSESPSLGAAATPIIMSRSPTDAKSRNSPRSNLK FRFDKLSHASSGAGH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001100398 |
| ORF Size | 2145 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001100398.2 |
| RefSeq Size | 6635 bp |
| RefSeq ORF | 2148 bp |
| Locus ID | 23108 |
| UniProt ID | Q684P5 |
| Gene Summary | This gene encodes a GTPase-activating protein that activates the small guanine-nucleotide-binding protein Rap1 in platelets. The protein interacts with synaptotagmin-like protein 1 and Rab27 and regulates secretion of dense granules from platelets at sites of endothelial damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214554 | RAP1GAP2 (Myc-DDK-tagged)-Human RAP1 GTPase activating protein 2 (RAP1GAP2), transcript variant 2 |
CNY 5,800.00 |
|
| RC214554L3 | Lenti-ORF clone of RAP1GAP2 (Myc-DDK-tagged)-Human RAP1 GTPase activating protein 2 (RAP1GAP2), transcript variant 2 |
CNY 7,700.00 |
|
| RC214554L4 | Lenti-ORF clone of RAP1GAP2 (mGFP-tagged)-Human RAP1 GTPase activating protein 2 (RAP1GAP2), transcript variant 2 |
CNY 7,700.00 |
|
| SC316825 | RAP1GAP2 (untagged)-Human RAP1 GTPase activating protein 2 (RAP1GAP2), transcript variant 2 |
CNY 13,780.00 |
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