WARS2 (NM_201263) Human Tagged ORF Clone
CAT#: RG213391
- TrueORF®
WARS2 (tGFP-tagged) - Human tryptophanyl tRNA synthetase 2, mitochondrial (WARS2), nuclear gene encoding mitochondrial protein, transcript variant 2
ORF Plasmid: DDK
"NM_201263" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | mtTrpRS; NEMMLAS; TrpRS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG213391 representing NM_201263
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGCACTCAATGCGGAAAGCGCGTGAGCGCTGGAGCTTCATCCGGGCACTTCATAAGGGATCCG CAGCTGCTCCCGCTCTCCAGAAAGACAGCAAGAAGCGAGTATTTTCCGGCATTCAACCTACAGGAATCCT CCACCTGGGCAATTACCTGGGAGCCATTGAGAGCTGGGTGAGGTTACAGGATGAATATGACTCTGTATTA TACAGCATTGTTGACCTCCACTCCATTACTGTCCCCCAAGACCCAGCTGTCCTTCGGCAGAGCATCCTGG ACATGACTGCTGTTCTTCTTGCCTGTGGCATAAACCCGGAAAAAAGCATCCTTTTCCAACAATCTCAGGT GTCTGAACACACACAATTAAGTTGGATCCTTTCCTGCATGGTCAGACTACCTCGATTACAACATTTACAT CAGTGGAAGGCAAAGACTACCAAGCAGAAGCACGATGGCACGGTGGGCCTGCTCACATACCCAGTACTCC AGGCAGCCGACATTCTGTTGTACAAGTCCACACACGTTCCTGTTGGGGAGGATCAAGTCCAGCACATGGA ACTAGTTCAGGATCTAGCACAAGGTTTCAACAAGAAGTATGGGGAGTTCTTTCCAGTGCCCGAGTCCATT CTCAGTATGTGTGTGTTGGTGTTTTTAACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213391 representing NM_201263
Red=Cloning site Green=Tags(s) MALHSMRKARERWSFIRALHKGSAAAPALQKDSKKRVFSGIQPTGILHLGNYLGAIESWVRLQDEYDSVL YSIVDLHSITVPQDPAVLRQSILDMTAVLLACGINPEKSILFQQSQVSEHTQLSWILSCMVRLPRLQHLH QWKAKTTKQKHDGTVGLLTYPVLQAADILLYKSTHVPVGEDQVQHMELVQDLAQGFNKKYGEFFPVPESI LSMCVLVFLT TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_201263 |
ORF Size | 660 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_201263.2, NP_957715.1 |
RefSeq Size | 2835 bp |
RefSeq ORF | 663 bp |
Locus ID | 10352 |
UniProt ID | Q9UGM6 |
Protein Families | Druggable Genome |
Protein Pathways | Aminoacyl-tRNA biosynthesis, Tryptophan metabolism |
Gene Summary | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. This gene encodes the mitochondrial tryptophanyl-tRNA synthetase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213391 | WARS2 (Myc-DDK-tagged)-Human tryptophanyl tRNA synthetase 2, mitochondrial (WARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3,990.00 |
|
RC213391L3 | Lenti-ORF clone of WARS2 (Myc-DDK-tagged)-Human tryptophanyl tRNA synthetase 2, mitochondrial (WARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
RC213391L4 | Lenti-ORF clone of WARS2 (mGFP-tagged)-Human tryptophanyl tRNA synthetase 2, mitochondrial (WARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 5,890.00 |
|
SC308001 | WARS2 (untagged)-Human tryptophanyl tRNA synthetase 2, mitochondrial (WARS2), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 3,990.00 |