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FBXO22 (NM_012170) Human Tagged ORF Clone
CAT＃: RG213115
- TrueORF®
FBXO22 (tGFP-tagged) - Human F-box protein 22 (FBXO22), transcript variant 2
ORF Plasmid: DDK
"NM_012170" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | FBX22; FISTC1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213115 representing NM_012170
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGGTAGGCTGCTGCGGCGAGTGCCGCGGCTCCTCCGTAGACCCGCGGAGCACCTTCGTGTTGA GTAACCTGGCGGAGGTGGTGGAGCGTGTGCTCACCTTCCTGCCCGCCAAGGCGTTGCTGCGGGTGGCCTG CGTGTGCCGCTTATGGAGGGAGTGTGTGCGCAGAGTATTGCGGACCCATCGGAGCGTAACCTGGATCTCC GCAGGCCTGGCGGAGGCCGGCCACCTGGAGGGGCATTGCTTGGTTCGCGTGGTAGCAGAGGAGCTTGAGA ATGTTCGCATCTTACCACATACAGTTCTTTACATGGCTGATTCAGAAACTTTCATTAGTCTGGAAGAGTG TCGTGGCCATAAGAGAGCAAGGAAAAGAACTAGTATGGAAACAGCACTTGCCCTTGAGAAGCTATTCCCC AAACAATGCCAAGTCCTTGGGATTGTGACCCCAGGAATTGTAGTGACTCCAATGGGATCAGGTAGCAATC GACCTCAGGAAATAGAAATTGGAGAATCTGGTTTTGCTTTATTATTCCCTCAAATTGAAGGAATAAAAAT ACAACCCTTTCATTTTATTAAGGATCCAAAGAATTTAACATTAGAAAGACATCAACTCACTGAAGTAGGT CTTTTAGATAACCCTGAACTTCGTGTGGTCCTTGTCTTTGGTTATAATTGCTGTAAGGTGGGAGCCAGTA ATTATCTGCAGCAAGTAGTCAGCACTTTCAGTGATATGAATATCATCTTGGCTGGAGGCCAGGTGGACAA CCTGTCATCACTGACTTCTGAAAAGTATGTCTTGTGTGCTTCTGATTTCGTCTGTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213115 representing NM_012170
Red=Cloning site Green=Tags(s) MEPVGCCGECRGSSVDPRSTFVLSNLAEVVERVLTFLPAKALLRVACVCRLWRECVRRVLRTHRSVTWIS AGLAEAGHLEGHCLVRVVAEELENVRILPHTVLYMADSETFISLEECRGHKRARKRTSMETALALEKLFP KQCQVLGIVTPGIVVTPMGSGSNRPQEIEIGESGFALLFPQIEGIKIQPFHFIKDPKNLTLERHQLTEVG LLDNPELRVVLVFGYNCCKVGASNYLQQVVSTFSDMNIILAGGQVDNLSSLTSEKYVLCASDFVCE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012170 |
| ORF Size | 828 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012170.3, NP_036302.1 |
| RefSeq Size | 1822 bp |
| RefSeq ORF | 831 bp |
| Locus ID | 26263 |
| UniProt ID | Q8NEZ5 |
| Domains | F-box |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and, as a transcriptional target of the tumor protein p53, is thought to be involved in degradation of specific proteins in response to p53 induction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213115 | FBXO22 (Myc-DDK-tagged)-Human F-box protein 22 (FBXO22), transcript variant 2 |
CNY 2,400.00 |
|
| RC213115L3 | Lenti ORF clone of Human F-box protein 22 (FBXO22), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213115L4 | Lenti ORF clone of Human F-box protein 22 (FBXO22), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC312773 | FBXO22 (untagged)-Human F-box protein 22 (FBXO22), transcript variant 2 |
CNY 6,270.00 |
