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PIM3 (NM_001001852) Human Tagged ORF Clone
CAT＃: RG212690
- TrueORF®
PIM3 (tGFP-tagged) - Human pim-3 oncogene (PIM3)
ORF Plasmid: DDK
"NM_001001852" in other vectors (6)
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CNY 5,200.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | pim-3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212690 representing NM_001001852
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTCTCCAAGTTCGGCTCCCTGGCGCACCTCTGCGGGCCCGGCGGCGTGGACCACCTCCCGGTGA AGATCCTGCAGCCAGCCAAGGCGGACAAGGAGAGCTTCGAGAAGGCGTACCAGGTGGGCGCCGTGCTGGG TAGCGGCGGCTTCGGCACGGTCTACGCGGGTAGCCGCATCGCCGACGGGCTCCCGGTGGCTGTGAAGCAC GTGGTGAAGGAGCGGGTGACCGAGTGGGGCAGCCTGGGCGGCGCGACCGTGCCCCTGGAGGTGGTGCTGC TGCGCAAGGTGGGCGCGGCGGGCGGCGCGCGCGGCGTCATCCGCCTGCTGGACTGGTTCGAGCGGCCCGA CGGCTTCCTGCTGGTGCTGGAGCGGCCCGAGCCGGCGCAGGACCTCTTCGACTTTATCACGGAGCGCGGC GCCCTGGACGAGCCGCTGGCGCGCCGCTTCTTCGCGCAGGTGCTGGCCGCCGTGCGCCACTGCCACAGCT GCGGGGTCGTGCACCGCGACATTAAGGACGAAAATCTGCTTGTGGACCTGCGCTCCGGAGAGCTCAAGCT CATCGACTTCGGTTCGGGTGCGCTGCTCAAGGACACGGTCTACACCGACTTCGACGGCACCCGAGTGTAC AGCCCCCCGGAGTGGATCCGCTACCACCGCTACCACGGGCGCTCGGCCACCGTGTGGTCGCTGGGCGTGC TTCTCTACGATATGGTGTGTGGGGACATCCCCTTCGAGCAGGACGAGGAGATCCTCCGAGGCCGCCTGCT CTTCCGGAGGAGGGTCTCTCCAGAGTGCCAGCAGCTGATCCGGTGGTGCCTGTCCCTGCGGCCCTCAGAG CGGCCGTCGCTGGATCAGATTGCGGCCCATCCCTGGATGCTGGGGGCTGACGGGGGCGCCCCGGAGAGCT GTGACCTGCGGCTGTGCACCCTCGACCCTGATGACGTGGCCAGCACCACGTCCAGCAGCGAGAGCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212690 representing NM_001001852
Red=Cloning site Green=Tags(s) MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKH VVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERG ALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVY SPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSE RPSLDQIAAHPWMLGADGGAPESCDLRLCTLDPDDVASTTSSSESL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001852 |
| ORF Size | 978 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001852.2, NP_001001852.1 |
| RefSeq Size | 2392 bp |
| RefSeq ORF | 981 bp |
| Locus ID | 415116 |
| UniProt ID | Q86V86 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212690 | PIM3 (Myc-DDK-tagged)-Human pim-3 oncogene (PIM3) |
CNY 3,600.00 |
|
| RC212690L1 | Lenti ORF clone of Human pim-3 oncogene (PIM3), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC212690L2 | Lenti ORF clone of Human pim-3 oncogene (PIM3), mGFP tagged |
CNY 6,000.00 |
|
| RC212690L3 | Lenti ORF clone of Human pim-3 oncogene (PIM3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212690L4 | Lenti ORF clone of Human pim-3 oncogene (PIM3), mGFP tagged |
CNY 5,890.00 |
|
| SC300319 | PIM3 (untagged)-Human pim-3 oncogene (PIM3) |
CNY 3,600.00 |
