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PRCP (NM_199418) Human Tagged ORF Clone
CAT＃: RG212658
- TrueORF®
PRCP (tGFP-tagged) - Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 2
ORF Plasmid: DDK
"NM_199418" in other vectors (6)
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CNY 4,940.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HUMPCP; PCP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212658 representing NM_199418
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGCCGAGCCCTCCTGCTCCTGCTTCTGTCTTTTCTGGCGCCCTGGGCCACCATAGCCCTCCGGC CGGCCTTAAGGGCCCTCGGCAGCCTACACTTGCCAACCAACCCCACATCCCTCCCGGCTGTAGCCAAGAA CTATTCGGTTCTCTACTTCCAACAGAAGGCCCTTGCTGCTGGGCAGTTGCACATATGTATTATCCAACTA AATCATTACAAGACTCCTCTGGTTGATCATTTTGGATTTAATACTGTGAAAACTTTTAATCAGCGGTACC TAGTAGCTGATAAATACTGGAAGAAAAATGGTGGATCAATACTTTTCTACACTGGTAATGAAGGGGACAT TATCTGGTTTTGTAATAACACGGGGTTCATGTGGGATGTGGCTGAGGAACTGAAAGCTATGTTGGTGTTT GCTGAACATCGATACTATGGAGAGTCTCTCCCCTTTGGTGACAACTCATTCAAGGATTCCAGACACTTGA ATTTCCTGACATCAGAACAAGCTCTGGCTGATTTTGCAGAGTTAATCAAACACTTGAAAAGAACAATCCC AGGAGCTGAAAATCAACCTGTCATTGCCATAGGAGGCTCCTATGGTGGCATGCTTGCCGCCTGGTTTAGG ATGAAATATCCTCATATGGTAGTTGGAGCTCTTGCAGCTTCTGCCCCTATCTGGCAGTTTGAGGATTTAG TACCTTGTGGTGTATTTATGAAGATCGTAACTACAGATTTTAGGAAAAGCGGTCCACATTGTTCAGAGAG CATCCACAGGTCCTGGGATGCCATTAATCGACTCTCAAATACTGGCAGTGGTTTGCAGTGGCTTACTGGA GCCCTTCACTTATGCAGCCCATTAACTTCTCAGGACATCCAACATTTGAAAGACTGGATCTCTGAAACCT GGGTGAATCTGGCAATGGTGGACTATCCTTATGCCTCTAACTTTTTACAGCCTTTGCCTGCTTGGCCTAT CAAGGTAGTGTGCCAGTATTTGAAAAATCCCAATGTATCTGATTCACTGCTGCTGCAGAATATTTTCCAA GCTCTGAATGTATATTACAATTATTCGGGCCAGGTGAAATGCCTGAATATTTCAGAGACAGCAACTAGCA GTCTGGGAACACTGGGTTGGAGCTATCAGGCCTGCACAGAAGTAGTCATGCCCTTTTGTACTAATGGTGT CGATGACATGTTTGAACCTCACTCATGGAACTTAAAGGAACTTTCTGATGACTGTTTTCAACAGTGGGGT GTGAGACCAAGGCCCTCCTGGATCACTACTATGTATGGAGGCAAAAACATTAGTTCACACACAAACATTG TTTTCAGCAATGGTGAACTAGACCCCTGGTCAGGAGGTGGAGTAACTAAGGATATCACAGACACTCTGGT TGCAGTCACCATCTCAGAGGGGGCCCACCACTTAGATCTCCGCACCAAGAATGCCTTGGATCCTATGTCT GTGCTGTTAGCCCGCTCCTTGGAAGTTAGACATATGAAGAATTGGATCAGAGATTTCTATGACAGTGCGG GAAAGCAGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212658 representing NM_199418
Red=Cloning site Green=Tags(s) MGRRALLLLLLSFLAPWATIALRPALRALGSLHLPTNPTSLPAVAKNYSVLYFQQKALAAGQLHICIIQL NHYKTPLVDHFGFNTVKTFNQRYLVADKYWKKNGGSILFYTGNEGDIIWFCNNTGFMWDVAEELKAMLVF AEHRYYGESLPFGDNSFKDSRHLNFLTSEQALADFAELIKHLKRTIPGAENQPVIAIGGSYGGMLAAWFR MKYPHMVVGALAASAPIWQFEDLVPCGVFMKIVTTDFRKSGPHCSESIHRSWDAINRLSNTGSGLQWLTG ALHLCSPLTSQDIQHLKDWISETWVNLAMVDYPYASNFLQPLPAWPIKVVCQYLKNPNVSDSLLLQNIFQ ALNVYYNYSGQVKCLNISETATSSLGTLGWSYQACTEVVMPFCTNGVDDMFEPHSWNLKELSDDCFQQWG VRPRPSWITTMYGGKNISSHTNIVFSNGELDPWSGGGVTKDITDTLVAVTISEGAHHLDLRTKNALDPMS VLLARSLEVRHMKNWIRDFYDSAGKQH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199418 |
| ORF Size | 1551 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199418.3 |
| RefSeq Size | 2224 bp |
| RefSeq ORF | 1554 bp |
| Locus ID | 5547 |
| UniProt ID | P42785 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Gene Summary | This gene encodes a member of the peptidase S28 family of serine exopeptidases. The encoded preproprotein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. A pseudogene of this gene has been identified on chromosome 2. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212658 | PRCP (Myc-DDK-tagged)-Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 2 |
CNY 3,848.00 |
|
| RC212658L1 | Lenti ORF clone of Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 2, Myc-DDK-tagged |
CNY 6,248.00 |
|
| RC212658L2 | Lenti ORF clone of Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 2, mGFP tagged |
CNY 6,370.00 |
|
| RC212658L3 | Lenti ORF clone of Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 2, Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC212658L4 | Lenti ORF clone of Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 2, mGFP tagged |
CNY 6,370.00 |
|
| SC128292 | PRCP (untagged)-Human prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 2 |
CNY 3,856.00 |
