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SMG7 (NM_173156) Human Tagged ORF Clone
CAT＃: RG212486
- TrueORF®
SMG7 (tGFP-tagged) - Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 1
ORF Plasmid: DDK
"NM_173156" in other vectors (4)
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CNY 12,450.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C1orf16; EST1C; SGA56M |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212486 representing NM_173156
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCTGCAGAGCGCGCAGTACCTCCGGCAGGCAGAAGTCCTGAAGGCTGACATGACAGATTCTAAGC TGGGTCCAGCTGAAGTCTGGACATCCAGGCAGGCTCTGCAGGACCTGTACCAGAAAATGCTAGTTACCGA TTTGGAATACGCTTTAGACAAGAAAGTAGAACAGGATCTCTGGAATCACGCCTTTAAGAATCAGATCACA ACACTGCAAGGCCAGGCAAAGAATCGAGCAAATCCGAATCGGAGTGAAGTTCAGGCAAACCTTTCTCTGT TCCTAGAGGCAGCTAGTGGCTTCTATACTCAGTTATTACAAGAACTGTGTACAGTATTTAATGTAGATTT ACCATGCCGTGTGAAGTCTTCCCAATTGGGAATTATCAGCAATAAACAGACGCATACCAGCGCCATAGTG AAGCCACAGTCTAGCTCCTGTTCCTATATCTGCCAGCACTGCCTCGTCCACCTTGGAGACATTGCTCGAT ACAGAAACCAGACCAGCCAGGCAGAGTCCTACTATAGGCATGCAGCTCAGCTTGTCCCCTCCAATGGTCA GCCTTATAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCATCTGACCACAATTTTCTACTACTGC AGAAGCATTGCTGTGAAGTTCCCTTTCCCAGCTGCCTCCACTAATCTGCAAAAAGCACTTTCTAAAGCAC TGGAAAGCCGAGATGAGGTGAAAACCAAGTGGGGTGTTTCTGACTTCATCAAGGCCTTTATTAAATTCCA CGGTCATGTGTACCTGAGTAAGAGCTTGGAAAAGTTGAGCCCTCTTCGAGAGAAATTGGAAGAACAGTTT AAGAGGCTGCTATTCCAAAAAGCTTTCAACTCTCAGCAGTTAGTTCATGTCACTGTCATTAACCTGTTTC AACTTCATCACCTTCGTGACTTTAGCAATGAAACCGAGCAGCACACTTATAGCCAAGATGAGCAGCTATG TTGGACACAGTTGCTGGCCCTCTTTATGTCTTTTCTTGGCATCCTGTGCAAGTGTCCTCTACAGAATGAG TCTCAGGAGGAGTCCTACAATGCCTATCCTCTTCCAGCAGTCAAGGTCTCCATGGACTGGCTAAGACTCA GACCCAGGGTCTTTCAGGAGGCAGTGGTGGATGAAAGACAGTACATTTGGCCCTGGTTGATTTCTCTTCT GAATAGTTTCCATCCCCATGAAGAGGACCTCTCAAGTATTAGTGCGACACCACTTCCAGAGGAGTTTGAA TTACAAGGATTTTTGGCATTGAGACCTTCTTTCAGGAACTTGGATTTTTCCAAAGGTCACCAGGGTATTA CAGGGGACAAAGAAGGCCAGCAACGACGAATACGACAGCAACGCTTGATCTCTATAGGCAAATGGATTGC TGATAATCAGCCAAGGCTGATTCAGTGTGAAAATGAGGTAGGGAAATTGTTGTTTATCACAGAAATCCCA GAATTAATACTGGAAGACCCCAGTGAAGCCAAAGAGAACCTCATTCTGCAAGAAACATCTGTGATAGAGT CGCTGGCTGCAGATGGGAGCCCAGGGCTAAAATCAGTGCTATCTACAAGCCGAAATTTAAGCAACAACTG TGACACAGGAGAGAAGCCAGTGGTTACCTTCAAAGAAAACATTAAGACACGAGAAGTGAACAGAGACCAA GGAAGAAGTTTTCCTCCCAAAGAGGTGAGAAGGGACTATAGCAAAGGAATAACTGTAACTAAGAATGATG GAAAGAAGGACAACAACAAGAGGAAAACTGAAACCAAGAAATGCACCTTAGAAAAGTTACAGGAAACAGG AAAGCAGAATGTGGCAGTGCAGGTAAAATCCCAGACAGAACTAAGAAAGACTCCAGTGTCTGAAGCCAGA AAAACACCTGTAACTCAAACCCCAACTCAAGCAAGTAACTCCCAGTTCATCCCCATTCATCACCCTGGAG CCTTCCCTCCTCTTCCCAGCAGGCCAGGGTTTCCGCCCCCAACATATGTTATCCCCCCGCCTGTGGCATT TTCTATGGGCTCAGGTTACACCTTCCCAGCTGGTGTTTCTGTCCCAGGAACCTTTCTTCAGCCTACAGCT CACTCTCCAGCAGGAAACCAGGTGCAAGCTGGGAAACAGTCCCACATTCCTTACAGCCAGCAACGGCCCT CTGGACCAGGGCCAATGAACCAGGGACCTCAACAATCACAGCCACCTTCCCAGCAACCCCTTACATCTTT ACCAGCTCAGCCAACAGCACAGTCTACAAGCCAGCTGCAGGTTCAAGCTCTAACTCAGCAACAACAATCC CCTACAAAAGCTGTGCCGGCTTTGGGGAAAAGCCCGCCTCACCACTCTGGATTCCAGCAGTATCAACAGG CAGATGCCTCCAAACAGCTGTGGAATCCCCCTCAGGTTCAAGGCCCATTAGGGAAAATTATGCCTGTGAA ACAGCCCTACTACCTTCAGACCCAAGACCCCATAAAACTGTTTGAGCCGTCATTGCAACCTCCTGTAATG CAGCAGCAGCCTCTAGAAAAAAAAATGAAGCCTTTTCCCATGGAGCCATATAACCATAATCCCTCAGAAG TCAAGGTCCCAGAATTCTACTGGGATTCTTCCTACAGCATGGCTGATAACAGATCTGTAATGGCACAGCA AGCAAACATAGACCGCAGGGGCAAACGGTCACCAGGAGTCTTCCGTCCAGAGCAGGATCCTGTACCCAGA ATGCCGTTTGAGAAATCCTTATTGGAGAAGCCCTCAGAGCTCATGTCACATTCATCCTCTTTCCTGTCCC TCACCGGATTCTCTCTCAATCAGGAAAGATACCCAAATAATAGTATGTTCAATGAGGTATATGGGAAAAA CCTGACATCCAGCTCCAAAGCAGAACTCAGTCCCTCAATGGCCCCCCAGGAAACATCTCTGTATTCCCTT TTTGAAGGGACTCCGTGGTCTCCATCACTTCCTGCCAGTTCAGATCATTCAACACCAGCCAGCCAGTCTC CTCATTCCTCTAACCCAAGCAGCCTACCCAGCTCTCCTCCAACACACAACCATAATTCTGTTCCATTCTC CAATTTTGGACCCATTGGGACTCCAGATAACAGGGATAGAAGGACTGCAGATCGGTGGAAAACTGATAAG CCAGCCATGGGTGGGTTTGGCATTGATTATCTCTCAGCAACGTCATCCTCTGAGAGCAGTTGGCATCAGG CCAGCACTCCGAGTGGCACCTGGACAGGCCATGGCCCTTCCATGGAGGATTCCTCTGCTGTCCTCATGGA AAGCCTAAAGTCTATCTGGTCCAGTTCCATGATGCATCCTGGACCTTCTGCTCTGGAGCAGCTGTTAATG CAGCAGAAGCAGAAACAGCAACGGGGACAAGGCACCATGAACCCTCCACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212486 representing NM_173156
Red=Cloning site Green=Tags(s) MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQIT TLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIV KPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYC RSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQF KRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNE SQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFE LQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIP ELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQ GRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEAR KTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTA HSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQS PTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVM QQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPR MPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSL FEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDK PAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLM QQKQKQQRGQGTMNPPH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173156 |
| ORF Size | 3411 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173156.3 |
| RefSeq Size | 5936 bp |
| RefSeq ORF | 3414 bp |
| Locus ID | 9887 |
| UniProt ID | Q92540 |
| Gene Summary | This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212486 | SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 1 |
CNY 11,310.00 |
|
| RC212486L3 | Lenti-ORF clone of SMG7 (Myc-DDK-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 1 |
CNY 13,210.00 |
|
| RC212486L4 | Lenti-ORF clone of SMG7 (mGFP-tagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 1 |
CNY 13,210.00 |
|
| SC315216 | SMG7 (untagged)-Human Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (SMG7), transcript variant 1 |
CNY 7,928.00 |
