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PHEX (NM_000444) Human Tagged ORF Clone
CAT＃: RG211248
- TrueORF®
PHEX (tGFP-tagged) - Human phosphate regulating endopeptidase homolog, X-linked (PHEX)
ORF Plasmid: DDK
"NM_000444" in other vectors (6)
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CNY 4,370.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HPDR; HPDR1; HYP; HYP1; LXHR; PEX; XLH |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211248 representing NM_000444
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGCAGAAACAGGGAGCAGCGTGGAGACTGGAAAGAAGGCCAACAGAGGCACTCGAATTGCCCTGG TCGTGTTTGTCGGTGGCACCCTAGTTCTGGGCACGATCCTCTTTCTAGTGAGTCAAGGTCTCTTAAGTCT CCAAGCTAAACAGGAGTACTGCCTGAAGCCAGAATGCATCGAAGCGGCTGCTGCCATCTTAAGTAAAGTA AATCTGTCTGTGGATCCTTGTGATAATTTCTTCCGGTTCGCTTGTGATGGCTGGATAAGCAATAATCCAA TTCCCGAAGATATGCCAAGCTATGGGGTTTATCCTTGGCTGAGACATAATGTTGACCTCAAGTTGAAGGA ACTTTTGGAGAAATCAATCAGTAGAAGGCGGGACACCGAAGCCATACAGAAAGCCAAAATCCTTTATTCA TCCTGCATGAATGAGAAAGCGATTGAAAAAGCAGATGCCAAGCCACTGCTACACATCCTACGGCATTCAC CTTTCCGCTGGCCCGTGCTTGAATCTAATATTGGCCCTGAAGGGGTTTGGTCAGAGAGAAAGTTCAGCCT TCTGCAGACACTTGCAACGTTTCGTGGTCAATACAGCAATTCTGTGTTCATCCGTTTGTATGTGTCCCCT GATGACAAAGCATCCAATGAACATATCTTGAAGCTGGACCAAGCAACACTCTCCCTGGCCGTGAGGGAAG ACTACCTTGATAACAGTACAGAAGCCAAGTCTTATCGGGATGCCCTTTACAAGTTCATGGTGGATACTGC CGTGCTTTTAGGAGCTAACAGTTCCAGAGCAGAGCATGACATGAAGTCAGTGCTCAGATTGGAAATTAAG ATAGCTGAGATAATGATTCCACATGAAAACCGAACCAGCGAGGCCATGTACAACAAAATGAACATTTCTG AACTGAGTGCTATGATTCCCCAGTTCGACTGGCTGGGCTACATCAAGAAGGTCATTGACACCAGACTCTA CCCCCATCTGAAAGACATCAGCCCCTCCGAGAATGTGGTGGTCCGCGTCCCGCAGTACTTTAAAGATTTG TTTAGGATATTAGGGTCTGAGAGAAAGAAGACCATTGCCAACTATTTGGTGTGGAGAATGGTTTATTCCA GAATTCCAAACCTTAGCAGGCGCTTTCAGTATAGATGGCTGGAATTCTCAAGGGTAATCCAGGGGACCAC AACTTTGCTGCCTCAATGGGACAAATGTGTAAACTTTATTGAAAGTGCCCTCCCTTATGTTGTTGGAAAG ATGTTTGTAGATGTGTACTTCCAGGAAGATAAGAAGGAAATGATGGAGGAATTGGTTGAGGGCGTTCGCT GGGCCTTTATTGACATGCTAGAGAAAGAAAATGAGTGGATGGATGCAGGAACGAAAAGGAAAGCCAAAGA AAAGGCGAGAGCTGTTTTGGCAAAAGTTGGCTATCCAGAGTTTATAATGAATGATACTCATGTTAATGAA GACCTCAAAGCTATCAAGTTTTCAGAAGCCGACTACTTTGGCAACGTCCTACAAACTCGCAAGTATTTAG CACAGTCTGATTTCTTCTGGCTAAGAAAAGCCGTTCCAAAAACAGAGTGGTTTACAAATCCGACGACTGT CAATGCCTTCTACAGTGCATCCACCAACCAGATCCGATTTCCAGCAGGAGAGCTCCAGAAGCCTTTCTTT TGGGGAACAGAATATCCTCGATCTCTGAGTTATGGTGCTATAGGAGTAATTGTCGGACATGAATTTACAC ATGGATTTGATAATAATGGTAGAAAATATGATAAAAATGGAAACCTGGATCCTTGGTGGTCTACTGAATC AGAAGAAAAGTTTAAGGAAAAAACAAAATGCATGATTAACCAGTATAGCAACTATTATTGGAAGAAAGCT GGCTTAAATGTCAAGGGGAAGAGGACCCTGGGAGAAAATATTGCTGATAATGGAGGCCTGCGGGAAGCTT TTAGGGCTTACAGGAAATGGATAAATGACAGAAGGCAGGGACTTGAGGAGCCTCTTCTACCAGGCATCAC ATTCACCAACAACCAGCTCTTCTTCCTGAGTTATGCTCATGTGAGGTGCAATTCCTACAGACCAGAAGCT GCCCGAGAACAAGTCCAAATTGGTGCTCACAGTCCCCCTCAGTTTAGGGTCAATGGTGCAATTAGTAACT TTGAAGAATTCCAGAAAGCTTTTAACTGTCCACCCAATTCCACGATGAACAGAGGCATGGACTCCTGCCG ACTCTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211248 representing NM_000444
Red=Cloning site Green=Tags(s) MEAETGSSVETGKKANRGTRIALVVFVGGTLVLGTILFLVSQGLLSLQAKQEYCLKPECIEAAAAILSKV NLSVDPCDNFFRFACDGWISNNPIPEDMPSYGVYPWLRHNVDLKLKELLEKSISRRRDTEAIQKAKILYS SCMNEKAIEKADAKPLLHILRHSPFRWPVLESNIGPEGVWSERKFSLLQTLATFRGQYSNSVFIRLYVSP DDKASNEHILKLDQATLSLAVREDYLDNSTEAKSYRDALYKFMVDTAVLLGANSSRAEHDMKSVLRLEIK IAEIMIPHENRTSEAMYNKMNISELSAMIPQFDWLGYIKKVIDTRLYPHLKDISPSENVVVRVPQYFKDL FRILGSERKKTIANYLVWRMVYSRIPNLSRRFQYRWLEFSRVIQGTTTLLPQWDKCVNFIESALPYVVGK MFVDVYFQEDKKEMMEELVEGVRWAFIDMLEKENEWMDAGTKRKAKEKARAVLAKVGYPEFIMNDTHVNE DLKAIKFSEADYFGNVLQTRKYLAQSDFFWLRKAVPKTEWFTNPTTVNAFYSASTNQIRFPAGELQKPFF WGTEYPRSLSYGAIGVIVGHEFTHGFDNNGRKYDKNGNLDPWWSTESEEKFKEKTKCMINQYSNYYWKKA GLNVKGKRTLGENIADNGGLREAFRAYRKWINDRRQGLEEPLLPGITFTNNQLFFLSYAHVRCNSYRPEA AREQVQIGAHSPPQFRVNGAISNFEEFQKAFNCPPNSTMNRGMDSCRLW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000444 |
| ORF Size | 2247 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_000444.6 |
| RefSeq Size | 2877 bp |
| RefSeq ORF | 2250 bp |
| Locus ID | 5251 |
| UniProt ID | P78562 |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| Gene Summary | The protein encoded by this gene is a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein is thought to be involved in bone and dentin mineralization and renal phosphate reabsorption. Mutations in this gene cause X-linked hypophosphatemic rickets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211248 | PHEX (Myc-DDK-tagged)-Human phosphate regulating endopeptidase homolog, X-linked (PHEX) |
CNY 8,232.00 |
|
| RC211248L1 | Lenti-ORF clone of PHEX (Myc-DDK-tagged)-Human phosphate regulating endopeptidase homolog, X-linked (PHEX) |
CNY 10,632.00 |
|
| RC211248L2 | Lenti-ORF clone of PHEX (mGFP-tagged)-Human phosphate regulating endopeptidase homolog, X-linked (PHEX) |
CNY 5,890.00 |
|
| RC211248L3 | Lenti-ORF clone of PHEX (Myc-DDK-tagged)-Human phosphate regulating endopeptidase homolog, X-linked (PHEX) |
CNY 5,890.00 |
|
| RC211248L4 | Lenti-ORF clone of PHEX (mGFP-tagged)-Human phosphate regulating endopeptidase homolog, X-linked (PHEX) |
CNY 5,890.00 |
|
| SC123993 | PHEX (untagged)-Human phosphate regulating endopeptidase homolog, X-linked (PHEX) |
CNY 8,240.00 |
