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EGR3 (NM_004430) Human Tagged ORF Clone
CAT＃: RG211140
- TrueORF®
EGR3 (tGFP-tagged) - Human early growth response 3 (EGR3), transcript variant 1
ORF Plasmid: DDK
"NM_004430" in other vectors (6)
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CNY 4,370.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | EGR-3; PILOT |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211140 representing NM_004430
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGGCAAACTCGCCGAGAAGCTGCCGGTGACCATGAGCAGTTTGCTAAACCAACTGCCTGACAATC TGTACCCCGAGGAGATCCCCAGCGCGCTCAACCTCTTCTCCGGCAGCAGCGACTCGGTAGTCCATTACAA TCAGATGGCTACAGAGAATGTAATGGACATCGGTCTGACCAACGAGAAGCCCAACCCGGAACTCTCTTAC TCCGGCTCCTTCCAGCCAGCCCCCGGCAACAAGACCGTGACCTACTTGGGAAAGTTCGCCTTCGACTCCC CTTCCAACTGGTGCCAGGACAACATCATTAGCCTCATGAGCGCCGGCATCTTGGGGGTGCCCCCGGCTTC AGGGGCGCTCAGCACGCAGACGTCCACGGCCAGCATGGTGCAGCCACCGCAGGGTGACGTGGAGGCCATG TATCCCGCGCTACCCCCCTACTCCAACTGCGGCGACCTCTACTCAGAGCCCGTGTCTTTCCACGACCCCC AGGGCAATCCCGGGCTCGCCTATTCCCCCCAGGATTACCAATCGGCCAAGCCGGCGTTGGACAGCAATCT CTTCCCCATGATTCCTGACTACAACCTCTACCACCACCCCAACGACATGGGCTCCATTCCGGAGCACAAG CCCTTCCAGGGCATGGACCCCATCCGGGTCAACCCGCCCCCTATTACCCCTCTGGAGACCATCAAGGCAT TCAAAGACAAGCAGATCCACCCGGGCTTTGGCAGCCTGCCCCAGCCGCCGCTCACCCTCAAGCCCATCCG GCCCCGCAAGTACCCCAACCGGCCTAGCAAGACACCGCTCCACGAACGGCCCCACGCGTGCCCGGCCGAG GGCTGCGACCGCCGTTTCAGCCGTTCGGACGAGCTGACCCGGCACCTGCGCATCCACACGGGCCACAAGC CCTTCCAGTGCCGGATCTGCATGCGGAGCTTCAGCCGCAGCGACCACCTCACCACTCACATCCGCACTCA TACGGGCGAGAAGCCCTTTGCCTGCGAGTTCTGCGGGCGCAAGTTTGCGCGCAGCGACGAGCGCAAGCGC CACGCCAAGATCCACCTCAAGCAAAAGGAGAAGAAGGCGGAGAAGGGCGGTGCACCCTCTGCATCCTCGG CGCCCCCCGTGTCGCTGGCCCCCGTGGTCACCACCTGCGCC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211140 representing NM_004430
Red=Cloning site Green=Tags(s) MTGKLAEKLPVTMSSLLNQLPDNLYPEEIPSALNLFSGSSDSVVHYNQMATENVMDIGLTNEKPNPELSY SGSFQPAPGNKTVTYLGKFAFDSPSNWCQDNIISLMSAGILGVPPASGALSTQTSTASMVQPPQGDVEAM YPALPPYSNCGDLYSEPVSFHDPQGNPGLAYSPQDYQSAKPALDSNLFPMIPDYNLYHHPNDMGSIPEHK PFQGMDPIRVNPPPITPLETIKAFKDKQIHPGFGSLPQPPLTLKPIRPRKYPNRPSKTPLHERPHACPAE GCDRRFSRSDELTRHLRIHTGHKPFQCRICMRSFSRSDHLTTHIRTHTGEKPFACEFCGRKFARSDERKR HAKIHLKQKEKKAEKGGAPSASSAPPVSLAPVVTTCA SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004430 |
| ORF Size | 1161 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004430.3 |
| RefSeq Size | 4342 bp |
| RefSeq ORF | 1164 bp |
| Locus ID | 1960 |
| UniProt ID | Q06889 |
| Gene Summary | This gene encodes a transcriptional regulator that belongs to the EGR family of C2H2-type zinc-finger proteins. It is an immediate-early growth response gene which is induced by mitogenic stimulation. The protein encoded by this gene participates in the transcriptional regulation of genes in controling biological rhythm. It may also play a role in a wide variety of processes including muscle development, lymphocyte development, endothelial cell growth and migration, and neuronal development. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Identification of proximal biomarkers of PKC agonism and evaluation of their role in HIV reactivation
,Vemula, SV;Maxwell, JW;Nefedov, A;Wan, BL;Steve, J;Newhard, W;Sanchez, RI;Tellers, D;Barnard, RJ;Blair, W;Hazuda, D;Webber, AL;Howell, BJ;,
Antiviral Res.
,PubMed ID 27889530
[Egr3]
|
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211140 | EGR3 (Myc-DDK-tagged)-Human early growth response 3 (EGR3), transcript variant 1 |
CNY 5,488.00 |
|
| RC211140L1 | Lenti ORF clone of Human early growth response 3 (EGR3), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC211140L2 | Lenti ORF clone of Human early growth response 3 (EGR3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC211140L3 | Lenti ORF clone of Human early growth response 3 (EGR3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211140L4 | Lenti ORF clone of Human early growth response 3 (EGR3), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| SC303482 | EGR3 (untagged)-Human early growth response 3 (EGR3), transcript variant 1 |
CNY 5,488.00 |
