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SLC9A3 (NM_004174) Human Tagged ORF Clone
CAT＃: RG210976
- TrueORF®
SLC9A3 (tGFP-tagged) - Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3)
ORF Plasmid: DDK
"NM_004174" in other vectors (6)
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CNY 7,712.00
CNY 7,890.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DIAR8; NHE-3; NHE3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210976 representing NM_004174
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGACTCGGGGCCCGGGGCCCCGACCGGGGGCTGCTGCTGGCGCTGGCGCTGGGCGGGCTGGCGC GGGCCGGGGGCGTCGAGGTGGAGCCCGGCGGCGCGCACGGCGAGAGCGGGGGCTTCCAGGTGGTCACCTT CGAGTGGGCCCACGTGCAGGATCCCTACGTCATCGCGCTCTGGATCCTCGTGGCCAGCTTGGCCAAGATC GGGTTCCACCTGTCCCACAAGGTCACCAGCGTGGTTCCCGAGAGCGCCCTGCTCATCGTGCTGGGCCTGG TGCTGGGCGGCATCGTCTGGGCGGCCGACCACATCGCGTCCTTCACACTGACGCCCACCGTCTTCTTCTT CTACCTGCTGCCCCCCATCGTGCTGGACGCCGGCTACTTCATGCCCAACCGCCTCTTCTTCGGCAACCTG GGGACCATCCTGTTGTACGCCGTCGTGGGTACCGTGTGGAACGCGGCCACCACCGGGCTGTCCCTCTACG GCGTCTTCCTCAGTGGGCTCATGGGCGACCTGCAGATTGGGCTGCTGGACTTCCTCCTGTTTGGCAGCCT CATGGCGGCTGTGGACCCGGTGGCCGTCCTGGCCGTGTTTGAGGAGGTCCATGTCAACGAGGTCCTGTTC ATCATCGTCTTCGGGGAGTCGCTGCTGAACGACGCAGTCACCGTGGTTCTGTACAATGTGTTTGAATCTT TCGTGGCGCTGGGAGGTGACAACGTGACTGGCGTGGACTGCGTGAAGGGCATAGTGTCCTTCTTCGTGGT GAGCCTGGGGGGCACGCTGGTGGGGGTGGTCTTCGCCTTCCTGCTGTCGCTGGTGACGCGCTTCACCAAG CATGTGCGTATCATCGAGCCCGGCTTCGTGTTCATCATCTCCTACCTGTCCTACCTGACGTCCGAGATGC TGTCGCTGTCGGCCATCCTCGCCATCACCTTCTGTGGCATCTGCTGTCAGAAGTATGTGAAGGCCAACAT CTCGGAGCAGTCGGCCACCACCGTGCGCTACACCATGAAGATGCTGGCCAGCAGCGCCGAGACCATCATC TTCATGTTCCTGGGTATCTCGGCCGTGAACCCGTTCATCTGGACCTGGAACACGGCCTTCGTGCTCCTGA CGCTGGTCTTCATCTCCGTGTACCGGGCCATCGGTGTGGTCCTGCAGACCTGGCTTCTGAACCGCTACCG CATGGTGCAGCTGGAGCCCATTGACCAGGTGGTCCTGTCCTACGGGGGCCTGCGCGGGGCCGTGGCCTTT GCCCTGGTGGTGCTTCTGGATGGAGACAAGGTCAAGGAGAAGAACCTGTTCGTCAGCACCACCATCATCG TAGTGTTCTTCACCGTCATCTTCCAGGGCCTGACCATCAAGCCTCTGGTGCAGTGGCTGAAGGTGAAGAG GAGCGAGCACCGGGAACCTCGGCTCAACGAGAAGCTGCACGGCCGCGCTTTCGACCACATCCTCTCGGCC ATCGAGGACATATCCGGACAGATCGGGCACAATTATCTCAGAGACAAGTGGTCCCACTTCGACAGGAAGT TCCTCAGCAGGGTCCTCATGAGACGGTCGGCCCAGAAGTCTCGAGACCGGATCCTGAATGTCTTCCACGA GCTGAACCTGAAGGATGCCATCAGCTACGTGGCTGAGGGAGAGCGCCGCGGGTCCCTGGCCTTCATCCGC TCCCCCAGCACCGACAACGTGGTCAACGTGGACTTCACGCCACGATCGTCCACCGTGGAGGCCTCTGTCT CCTACCTCCTGAGAGAAAATGTCAGCGCTGTCTGCCTGGACATGCAGTCTCTGGAGCAGCGACGGCGGAG CATCCGGGACGCGGAGGACATGGTCACGCACCACACGCTACAGCAGTACCTGTACAAGCCGCGGCAGGAG TACAAGCATCTGTACAGCCGACACGAGCTCACGCCCACGGAGGACGAGAAACAGGACCGGGAAATCTTCC ACAGGACCATGCGGAAGCGCCTGGAGTCCTTCAAGTCGACCAAGCTGGGGCTCAACCAGAACAAGAAGGC AGCCAAGCTGTACAAGCGGGAGCGTGCCCAGAAGCGGAGAAACAGCAGCATCCCCAATGGGAAGCTGCCC ATGGAGAGCCCTGCGCAGAATTTCACCATCAAGGAGAAAGACTTGGAACTTTCAGACACCGAGGAGCCCC CCAACTATGATGAGGAGATGAGTGGGGGGATCGAGTTCCTGGCTAGTGTCACCAAGGACACAGCGTCCGA CTCCCCTGCAGGAATTGACAACCCTGTGTTTTCTCCGGACGAGGCCCTGGACCGCAGCCTCCTGGCCAGG CTGCCGCCCTGGCTGTCTCCCGGGGAGACGGTGGTCCCCTCGCAGAGGGCCCGCACGCAGATTCCCTACT CTCCCGGCACCTTCCGCCGCCTGATGCCCTTCCGCCTCAGCAGCAAGTCCGTGGACTCCTTCCTGCAGGC AGACGGCCCCGAGGAGCGGCCCCCCGCCGCCCTCCCCGAGTCCACACACATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210976 representing NM_004174
Red=Cloning site Green=Tags(s) MWGLGARGPDRGLLLALALGGLARAGGVEVEPGGAHGESGGFQVVTFEWAHVQDPYVIALWILVASLAKI GFHLSHKVTSVVPESALLIVLGLVLGGIVWAADHIASFTLTPTVFFFYLLPPIVLDAGYFMPNRLFFGNL GTILLYAVVGTVWNAATTGLSLYGVFLSGLMGDLQIGLLDFLLFGSLMAAVDPVAVLAVFEEVHVNEVLF IIVFGESLLNDAVTVVLYNVFESFVALGGDNVTGVDCVKGIVSFFVVSLGGTLVGVVFAFLLSLVTRFTK HVRIIEPGFVFIISYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYTMKMLASSAETII FMFLGISAVNPFIWTWNTAFVLLTLVFISVYRAIGVVLQTWLLNRYRMVQLEPIDQVVLSYGGLRGAVAF ALVVLLDGDKVKEKNLFVSTTIIVVFFTVIFQGLTIKPLVQWLKVKRSEHREPRLNEKLHGRAFDHILSA IEDISGQIGHNYLRDKWSHFDRKFLSRVLMRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIR SPSTDNVVNVDFTPRSSTVEASVSYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQE YKHLYSRHELTPTEDEKQDREIFHRTMRKRLESFKSTKLGLNQNKKAAKLYKRERAQKRRNSSIPNGKLP MESPAQNFTIKEKDLELSDTEEPPNYDEEMSGGIEFLASVTKDTASDSPAGIDNPVFSPDEALDRSLLAR LPPWLSPGETVVPSQRARTQIPYSPGTFRRLMPFRLSSKSVDSFLQADGPEERPPAALPESTHM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004174 |
| ORF Size | 2502 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004174.1, NP_004165.1 |
| RefSeq Size | 2584 bp |
| RefSeq ORF | 2505 bp |
| Locus ID | 6550 |
| UniProt ID | P48764 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | The protein encoded by this gene is an epithelial brush border Na/H exchanger that uses an inward sodium ion gradient to expel acids from the cell. Defects in this gene are a cause of congenital secretory sodium diarrhea. Pseudogenes of this gene exist on chromosomes 10 and 22. [provided by RefSeq, Mar 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Phospho-NHE3 forms membrane patches and interacts with beta-actin to sense and maintain constant direction during cell migration
,Perike, S;Ozkucur, N;Sharma, P;Staroske, W;Bläsche, R;Barth, K;Funk, RH;,
Exp. Cell Res.
,PubMed ID 24657527
[SLC9A3]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210976 | SLC9A3 (Myc-DDK-tagged)-Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3) |
CNY 6,112.00 |
|
| RC210976L1 | Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3), Myc-DDK-tagged |
CNY 8,512.00 |
|
| RC210976L2 | Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3), mGFP tagged |
CNY 9,030.00 |
|
| RC210976L3 | Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC210976L4 | Lenti ORF clone of Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3), mGFP tagged |
CNY 8,512.00 |
|
| SC303446 | SLC9A3 (untagged)-Human solute carrier family 9 (sodium/hydrogen exchanger), member 3 (SLC9A3) |
CNY 6,120.00 |
