LHX2 (NM_004789) Human Tagged ORF Clone

CAT#: RG210786

  • TrueORF®

LHX2 (tGFP-tagged) - Human LIM homeobox 2 (LHX2)

ORF Plasmid: DDK tGFP



  "NM_004789" in other vectors (6)

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CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


LHX2 mouse monoclonal antibody,clone OTI5C7
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms hLhx2; LH2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG210786 representing NM_004789
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTTCCACAGTCTGTCGGGCCCCGAGGTGCACGGGGTCATCGACGAGATGGACCGCAGGGCCAAGA
GCGAGGCTCCCGCCATCAGCTCCGCCATCGACCGCGGCGACACCGAGACGACCATGCCGTCCATCAGCAG
TGACCGCGCCGCGCTGTGCGCCGGCTGCGGGGGCAAGATCTCGGACCGCTACTACCTGCTGGCGGTGGAC
AAGCAGTGGCACATGCGCTGCCTCAAGTGCTGCGAGTGCAAGCTCAACCTGGAGTCGGAGCTCACCTGTT
TCAGCAAGGACGGTAGCATCTACTGCAAGGAAGACTACTACAGGCGCTTCTCTGTGCAGCGCTGCGCCCG
CTGCCACCTGGGCATCTCGGCCTCGGAGATGGTGATGCGCGCTCGGGACTTGGTTTATCACCTCAACTGC
TTCACGTGCACCACGTGTAACAAGATGCTGACCACGGGCGACCACTTCGGCATGAAGGACAGCCTGGTCT
ACTGCCGCTTGCACTTCGAGGCGCTGCTGCAGGGCGAGTACCCCGCACACTTCAACCATGCCGACGTGGC
AGCGGCGGCCGCTGCAGCCGCGGCGGCCAAGAGCGCGGGGCTGGGCGCAGCAGGGGCCAACCCTCTGGGT
CTTCCCTACTACAATGGCGTGGGCACTGTGCAGAAGGGGCGGCCGAGGAAACGTAAGAGCCCGGGCCCCG
GTGCGGATCTGGCGGCCTACAACGCTGCGCTAAGCTGCAACGAAAACGACGCAGAGCACCTGGACCGTGA
CCAGCCATACCCGAGCAGCCAGAAGACCAAGCGCATGCGCACGTCCTTCAAGCACCACCAGCTTCGGACC
ATGAAGTCTTACTTTGCCATTAACCACAACCCCGACGCCAAGGACTTGAAGCAGCTCGCGCAAAAGACGG
GCCTCACCAAGCGGGTCCTCCAGGTCTGGTTCCAGAACGCCCGAGCCAAGTTCAGGCGCAACCTCTTACG
GCAGGAAAACACGGGCGTGGACAAGTCGACAGACGCGGCGCTGCAGACAGGGACGCCATCGGGCCCGGCC
TCGGAGCTCTCCAACGCCTCGCTCAGCCCCTCCAGCACGCCCACCACCCTGACAGACTTGACTAGCCCCA
CCCTGCCAACTGTGACGTCCGTCTTAACTTCTGTGCCTGGCAACCTGGAGGGCCATGAGCCTCACAGCCC
CTCACAAACGACTCTTACCAACCTTTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG210786 representing NM_004789
Red=Cloning site Green=Tags(s)

MLFHSLSGPEVHGVIDEMDRRAKSEAPAISSAIDRGDTETTMPSISSDRAALCAGCGGKISDRYYLLAVD
KQWHMRCLKCCECKLNLESELTCFSKDGSIYCKEDYYRRFSVQRCARCHLGISASEMVMRARDLVYHLNC
FTCTTCNKMLTTGDHFGMKDSLVYCRLHFEALLQGEYPAHFNHADVAAAAAAAAAAKSAGLGAAGANPLG
LPYYNGVGTVQKGRPRKRKSPGPGADLAAYNAALSCNENDAEHLDRDQPYPSSQKTKRMRTSFKHHQLRT
MKSYFAINHNPDAKDLKQLAQKTGLTKRVLQVWFQNARAKFRRNLLRQENTGVDKSTDAALQTGTPSGPA
SELSNASLSPSSTPTTLTDLTSPTLPTVTSVLTSVPGNLEGHEPHSPSQTTLTNLF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004789
ORF Size 1218 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004789.4
RefSeq Size 2416 bp
RefSeq ORF 1221 bp
Locus ID 9355
UniProt ID P50458
Domains homeobox, LIM
Protein Families Transcription Factors
Gene Summary This gene encodes a protein belonging to a large protein family, members of which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein may function as a transcriptional regulator. The protein can recapitulate or rescue phenotypes in Drosophila caused by a related protein, suggesting conservation of function during evolution. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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