DNA Polymerase beta (POLB) (NM_002690) Human Tagged ORF Clone
CAT#: RG210765
- TrueORF®
POLB (tGFP-tagged) - Human polymerase (DNA directed), beta (POLB)
ORF Plasmid: DDK
"NM_002690" in other vectors (7)
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CNY 5,256.00
Cited in 4 publications. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210765 representing NM_002690
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCAAACGGAAGGCGCCGCAGGAGACTCTCAACGGGGGAATCACCGACATGCTCACAGAACTCGCAA ACTTTGAGAAGAACGTGAGCCAAGCTATCCACAAGTACAATGCTTACAGAAAAGCAGCATCTGTTATAGC AAAATACCCACACAAAATAAAGAGTGGAGCTGAAGCTAAGAAATTGCCTGGAGTAGGAACAAAAATTGCT GAAAAGATTGATGAGTTTTTAGCAACTGGAAAATTACGTAAACTGGAAAAGATTCGGCAGGATGATACGA GTTCATCCATCAATTTCCTGACTCGAGTTAGTGGCATTGGTCCATCTGCTGCAAGGAAGTTTGTAGATGA AGGAATTAAAACACTAGAAGATCTCAGAAAAAATGAAGATAAATTGAACCATCATCAGCGAATTGGGCTG AAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGAAGAGATGTTACAAATGCAAGATATTGTACTAA ATGAAGTTAAAAAAGTGGATTCTGAATACATTGCTACAGTCTGTGGCAGTTTCAGAAGAGGTGCAGAGTC CAGTGGTGACATGGATGTTCTCCTGACCCATCCCAGCTTCACTTCAGAATCAACCAAACAGCCAAAACTG TTACATCAGGTTGTGGAGCAGTTACAAAAGGTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAA AGTTCATGGGTGTTTGCCAGCTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATAT CAGGTTGATACCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATATTTTCAATAAG AATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACCATCCGTCCCTTGGGAGTCA CTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAAGACATCTTTGATTACATCCAGTGGAAATA CCGGGAACCCAAGGACCGGAGCGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210765 representing NM_002690
Red=Cloning site Green=Tags(s) MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIA EKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGL KYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKL LHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNK NMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002690 |
ORF Size | 1005 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002690.3 |
RefSeq Size | 1259 bp |
RefSeq ORF | 1008 bp |
Locus ID | 5423 |
UniProt ID | P06746 |
Domains | POLXc, HhH1 |
Protein Families | Druggable Genome |
Protein Pathways | Base excision repair |
Gene Summary | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011] |
Citations (4)
The use of this cDNA Clones has been cited in the following citations: |
---|
Monitoring DNA polymerase β mitochondrial localization and dynamics.
,null,
DNA repair
,PubMed ID 35717762
[POLB]
|
Lysines in the lyase active site of DNA polymerase β destabilize non-specific DNA binding, facilitating searching and DNA gap recognition
,Howard, MJ;Horton, JK;Zhao, ML;Wilson, SH;,
J. Biol. Chem.
,PubMed ID 32647014
[POLB]
|
DNA Polymerase Beta Participates in Mitochondrial DNA Repair
,null,
Molecular and Cellular Biology
,PubMed ID 28559431
[POLB]
|
Role of the oxidized form of XRCC1 in protection against extreme oxidative stress
,Horton, JK;Seddon, HJ;Zhao, ML;Gassman, NR;Janoshazi, AK;Stefanick, DF;Wilson, SH;,
Free Radic. Biol. Med
,PubMed ID 28179111
[POLB]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210765 | POLB (Myc-DDK-tagged)-Human polymerase (DNA directed), beta (POLB) |
CNY 3,656.00 |
|
RC210765L1 | Lenti ORF clone of Human polymerase (DNA directed), beta (POLB), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC210765L2 | Lenti ORF clone of Human polymerase (DNA directed), beta (POLB), mGFP tagged |
CNY 5,890.00 |
|
RC210765L3 | Lenti ORF clone of Human polymerase (DNA directed), beta (POLB), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210765L4 | Lenti ORF clone of Human polymerase (DNA directed), beta (POLB), mGFP tagged |
CNY 5,890.00 |
|
SC118518 | POLB (untagged)-Human polymerase (DNA directed), beta (POLB) |
CNY 3,656.00 |
|
SC322059 | POLB (untagged)-Human polymerase (DNA directed), beta (POLB) |
CNY 3,656.00 |
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