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VRK1 (NM_003384) Human Tagged ORF Clone
CAT＃: RG210764
- TrueORF®
VRK1 (tGFP-tagged) - Human vaccinia related kinase 1 (VRK1)
ORF Plasmid: DDK
"NM_003384" in other vectors (7)
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CNY 5,256.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PCH1; PCH1A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210764 representing NM_003384
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGTGTAAAAGCAGCTCAAGCTGGAAGACAGAGCTCTGCAAAGAGACATCTTGCAGAACAATTTG CAGTTGGAGAGATAATAACTGACATGGCAAAAAAGGAATGGAAAGTAGGATTACCCATTGGCCAAGGAGG CTTTGGCTGTATATATCTTGCTGATATGAATTCTTCAGAGTCAGTTGGCAGTGATGCACCTTGTGTTGTA AAAGTGGAACCCAGTGACAATGGACCTCTTTTTACTGAATTAAAGTTCTACCAACGAGCTGCAAAACCAG AGCAAATTCAGAAATGGATTCGTACCCGTAAGCTGAAGTACCTGGGTGTTCCTAAGTATTGGGGGTCTGG TCTACATGACAAAAATGGAAAAAGTTACAGGTTTATGATAATGGATCGCTTTGGGAGTGACCTTCAGAAA ATATATGAAGCAAATGCCAAAAGGTTTTCTCGGAAAACTGTCTTGCAGCTAAGCTTAAGAATTCTGGATA TTCTGGAATATATTCACGAGCATGAGTATGTGCATGGAGATATCAAGGCCTCAAATCTTCTTCTGAACTA CAAGAATCCTGACCAGGTGTACTTGGTAGATTATGGCCTTGCTTATCGGTACTGCCCAGAAGGAGTTCAT AAAGAATACAAAGAAGACCCCAAAAGATGTCACGATGGCACTATTGAATTCACGAGCATCGATGCACACA ATGGCGTGGCCCCATCAAGACGTGGTGATTTGGAAATACTTGGTTATTGCATGATCCAATGGCTTACTGG CCATCTTCCTTGGGAGGATAATTTGAAAGATCCTAAATATGTTAGAGATTCCAAAATTAGATACAGAGAA AATATTGCAAGTTTGATGGACAAATGTTTTCCTGAGAAAAACAAACCAGGTGAAATTGCCAAATACATGG AAACAGTGAAATTACTAGACTACACTGAAAAACCTCTTTATGAAAATTTACGTGACATTCTTTTGCAAGG ACTAAAAGCTATAGGAAGTAAGGATGATGGCAAATTGGACCTCAGTGTTGTGGAGAATGGAGGTTTGAAA GCAAAAACAATAACAAAGAAGCGAAAGAAAGAAATTGAAGAAAGCAAGGAACCTGGTGTTGAAGATACGG AATGGTCAAACACACAGACAGAGGAGGCCATACAGACCCGTTCAAGAACCAGAAAGAGAGTCCAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210764 representing NM_003384
Red=Cloning site Green=Tags(s) MPRVKAAQAGRQSSAKRHLAEQFAVGEIITDMAKKEWKVGLPIGQGGFGCIYLADMNSSESVGSDAPCVV KVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTRKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQK IYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLNYKNPDQVYLVDYGLAYRYCPEGVH KEYKEDPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLTGHLPWEDNLKDPKYVRDSKIRYRE NIASLMDKCFPEKNKPGEIAKYMETVKLLDYTEKPLYENLRDILLQGLKAIGSKDDGKLDLSVVENGGLK AKTITKKRKKEIEESKEPGVEDTEWSNTQTEEAIQTRSRTRKRVQK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003384 |
| ORF Size | 1188 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003384.3 |
| RefSeq Size | 1720 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 7443 |
| UniProt ID | Q99986 |
| Domains | pkinase, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210764 | VRK1 (Myc-DDK-tagged)-Human vaccinia related kinase 1 (VRK1) |
CNY 3,656.00 |
|
| RC210764L1 | Lenti ORF clone of Human vaccinia related kinase 1 (VRK1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210764L2 | Lenti ORF clone of Human vaccinia related kinase 1 (VRK1), mGFP tagged |
CNY 5,890.00 |
|
| RC210764L3 | Lenti ORF clone of Human vaccinia related kinase 1 (VRK1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210764L4 | Lenti ORF clone of Human vaccinia related kinase 1 (VRK1), mGFP tagged |
CNY 5,890.00 |
|
| SC117994 | VRK1 (untagged)-Human vaccinia related kinase 1 (VRK1) |
CNY 3,656.00 |
|
| SC323490 | VRK1 (untagged)-Kinase deficient mutant (K71M) of Human vaccinia related kinase 1 (VRK1) |
CNY 3,656.00 |
