CST8 (NM_005492) Human Tagged ORF Clone
CAT#: RG210130
- TrueORF®
CST8 (tGFP-tagged) - Human cystatin 8 (cystatin-related epididymal specific) (CST8)
ORF Plasmid: DDK
"NM_005492" in other vectors (6)
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CNY 2,800.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CRES; CTES5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210130 representing NM_005492
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCAGGTGCCGGTGGCTCTCCCTGATCCTCCTCACCATTCCCCTGGCCCTGGTGGCCAGGAAAGACC CAAAAAAGAATGAGACGGGGGTGCTGAGGAAATTAAAACCCGTCAATGCCTCAAATGCCAACGTGAAGCA GTGTCTGTGGTTTGCCATGCAAGAATACAACAAAGAGAGCGAGGACAAGTATGTCTTCCTGGTGGTCAAG ACACTGCAAGCCCAGCTTCAGGTCACAAATCTTCTGGAATACCTTATTGATGTAGAAATTGCCCGCAGCG ATTGCAGAAAGCCTTTAAGCACTAATGAAATCTGCGCCATTCAAGAAAACTCCAAGCTGAAAAGGAAATT AAGCTGCAGCTTTTTGGTAGGAGCACTTCCCTGGAATGGTGAATTCACTGTGATGGAGAAAAAGTGTGAA GATGCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210130 representing NM_005492
Red=Cloning site Green=Tags(s) MPRCRWLSLILLTIPLALVARKDPKKNETGVLRKLKPVNASNANVKQCLWFAMQEYNKESEDKYVFLVVK TLQAQLQVTNLLEYLIDVEIARSDCRKPLSTNEICAIQENSKLKRKLSCSFLVGALPWNGEFTVMEKKCE DA TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005492 |
ORF Size | 426 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005492.1 |
RefSeq Size | 895 bp |
RefSeq ORF | 429 bp |
Locus ID | 10047 |
UniProt ID | O60676 |
Protein Families | Secreted Protein |
Gene Summary | The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a protein similar to type 2 cystatins. The encoded protein exhibits highly tissue-specific expression in the reproductive tract, suggesting implicit roles in reproduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210130 | CST8 (Myc-DDK-tagged)-Human cystatin 8 (cystatin-related epididymal specific) (CST8) |
CNY 1,200.00 |
|
RC210130L1 | Lenti ORF clone of Human cystatin 8 (cystatin-related epididymal specific) (CST8), Myc-DDK-tagged |
CNY 3,600.00 |
|
RC210130L2 | Lenti ORF clone of Human cystatin 8 (cystatin-related epididymal specific) (CST8), mGFP tagged |
CNY 5,890.00 |
|
RC210130L3 | Lenti ORF clone of Human cystatin 8 (cystatin-related epididymal specific) (CST8), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210130L4 | Lenti ORF clone of Human cystatin 8 (cystatin-related epididymal specific) (CST8), mGFP tagged |
CNY 5,890.00 |
|
SC303654 | CST8 (untagged)-Human cystatin 8 (cystatin-related epididymal specific) (CST8) |
CNY 3,990.00 |