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ADAMTS7 (NM_014272) Human Tagged ORF Clone
CAT＃: RG208574
- TrueORF®
ADAMTS7 (tGFP-tagged) - Human ADAM metallopeptidase with thrombospondin type 1 motif, 7 (ADAMTS7)
ORF Plasmid: DDK
"NM_014272" in other vectors (2)
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CNY 18,600.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADAM-TS 7; ADAM-TS7; ADAMTS-7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208574 representing NM_014272
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGCGGCCCCAGTCCCCGCAGCCCCGCGCCTTTGCTGCGCCCCCTCCTCCTGCTCCTCTGCGCTC TGGCTCCCGGCGCCCCCGGACCCGCACCAGGACGTGCAACCGAGGGCCGGGCGGCACTGGACATCGTGCA CCCGGTTCGAGTCGACGCGGGGGGCTCCTTCCTGTCCTACGAGCTGTGGCCCCGCGCACTGCGCAAGCGG GATGTATCTGTGCGCCGAGACGCGCCCGCCTTCTACGAGCTACAATACCGCGGGCGCGAGCTGCGCTTCA ACCTGACCGCCAATCAGCACCTGCTGGCGCCCGGCTTTGTGAGCGAGACGCGGCGGCGCGGCGGCCTGGG CCGCGCGCACATCCGGGCCCACACCCCGGCCTGCCATCTGCTTGGCGAGGTGCAGGACCCTGAGCTCGAG GGTGGCCTGGCGGCCATCAGCGCCTGCGACGGCCTGAAAGGTGTGTTCCAGCTCTCCAACGAGGACTACT TCATTGAGCCCCTGGACAGTGCCCCGGCCCGGCCTGGCCACGCCCAGCCCCATGTGGTGTACAAGCGTCA GGCCCCGGAGAGGCTGGCACAGCGGGGTGATTCCAGTGCTCCAAGCACCTGTGGAGTGCAAGTGTACCCA GAGCTGGAGTCTCGACGGGAGCGTTGGGAGCAGCGGCAGCAGTGGCGGCGGCCACGGCTGAGGCGTCTAC ACCAGCGGTCGGTCAGCAAAGAGAAGTGGGTGGAGACCCTGGTGGTAGCTGATGCCAAAATGGTGGAGTA CCACGGACAGCCGCAGGTTGAGAGCTATGTGCTGACCATCATGAACATGGTGGCTGGCCTGTTTCATGAC CCCAGCATTGGGAACCCCATCCACATCACCATTGTGCGCCTGGTCCTGCTGGAAGATGAGGAGGAGGACC TAAAGATCATGCACCATGCAGACAACACCCTGAAGAGCTTCTGCAAGTGGCAGAAAAGCATCAACATGAA GGGGGATGCCCATCCCCTGCACCATGACACTGCCATCCTGCTCACCAGAAAGGACCTGTGTGCAGCCATG AACCGGCCCTGTGAGACCCTGGGACTGTCCCATGTGGCGGGCATGTGCCAGCCGCACCGCAGCTGCAGCA TCAACGAGGACACGGGCCTGCCGCTGGCCTTCACTGTAGCCCACGAGCTCGGGCACAGTTTTGGCATTCA GCATGACGGAAGCGGCAATGACTGTGAGCCCGTTGGGAAACGACCTTTCATCATGTCTCCACAGCTCCTG TACGACGCCGCTCCCCTCACCTGGTCCCGCTGCAGCCGCCAGTATATCACCAGGTTCCTTGACCGTGGGT GGGGCCTGTGCCTGGACGACCCTCCTGCCAAGGACATTATCGACTTCCCCTCGGTGCCACCTGGCGTCCT CTATGATGTAAGCCACCAGTGCCGCCTCCAGTACGGGGCCTACTCTGCCTTCTGCGAGGACATGGATAAT GTCTGCCACACACTCTGGTGCTCTGTGGGGACCACCTGTCACTCCAAGCTGGATGCAGCTGTGGACGGCA CCCGGTGTGGGGAGAATAAGTGGTGTCTCAGTGGGGAGTGCGTACCCGTGGGCTTCCGGCCCGAGGCCGT GGATGGTGGCTGGTCTGGCTGGAGCGCCTGGTCCATCTGCTCACGGAGCTGTGGCATGGGCGTACAGAGC GCCGAGCGGCAGTGCACGCAGCCTACGCCCAAATACAAAGGCAGATACTGTGTGGGTGAGCGCAAGCGCT TCCGCCTCTGCAACCTGCAGGCCTGCCCTGCTGGCCGCCCCTCCTTCCGCCACGTCCAGTGCAGCCACTT TGACGCTATGCTCTACAAGGGCCAGCTGCACACATGGGTGCCCGTGGTCAATGACGTGAACCCCTGCGAG CTGCACTGCCGGCCCGCGAATGAGTACTTTGCCGAGAAGCTGCGGGACGCCGTGGTCGATGGCACCCCCT GCTACCAGGTCCGAGCCAGCCGGGACCTCTGCATCAACGGCATCTGTAAGAACGTGGGCTGTGACTTCGA GATTGACTCCGGTGCTATGGAGGACCGCTGTGGTGTGTGCCACGGCAACGGCTCCACCTGCCACACCGTG AGCGGGACCTTCGAGGAGGCCGAGGGCCTGGGGTATGTGGATGTGGGGCTGATCCCAGCGGGCGCACGCG AGATCCGCATCCAAGAGGTTGCCGAGGCTGCCAACTTCCTGGCACTGCGGAGCGAGGACCCGGAGAAGTA CTTCCTCAATGGTGGCTGGACCATCCAGTGGAACGGGGACTACCAGGTGGCAGGGACCACCTTCACATAC GCACGCAGGGGCAACTGGGAGAACCTCACGTCCCCGGGTCCCACCAAGGAGCCTGTCTGGATCCAGCTGC TGTTCCAGGAGAGCAACCCTGGGGTGCACTACGAGTACACCATCCACAGGGAGGCAGGTGGCCACGACGA GGTCCCGCCGCCCGTGTTCTCCTGGCATTATGGGCCCTGGACCAAGTGCACAGTCACCTGCGGCAGAGGT GTGCAGAGACAGAATGTGTACTGCTTGGAGCGGCAGGCAGGGCCCGTGGACGAGGAGCACTGTGACCCCC TGGGCCGGCCTGATGACCAACAGAGGAAGTGCAGCGAGCAGCCCTGCCCTGCCAGGTGGTGGGCAGGTGA GTGGCAGCTGTGCTCCAGCTCCTGCGGGCCTGGGGGCCTCTCCCGCCGGGCCGTGCTCTGCATCCGCAGC GTGGGGCTGGATGAGCAGAGCGCCCTGGAGCCACCCGCCTGTGAACACCTTCCCCGGCCCCCTACTGAAA CCCCTTGCAACCGCCATGTACCCTGTCCGGCCACCTGGGCTGTGGGGAACTGGTCTCAGTGCTCAGTGAC ATGTGGGGAGGGCACTCAGCGCCGAAATGTCCTCTGCACCAATGACACCGGTGTCCCCTGTGACGAGGCC CAGCAGCCAGCCAGCGAAGTCACCTGCTCTCTGCCACTCTGTCGGTGGCCCCTGGGCACACTGGGCCCTG AAGGCTCAGGCAGCGGCTCCTCCAGCCACGAGCTCTTCAACGAGGCTGACTTCATCCCGCACCACCTGGC CCCACGCCCTTCACCCGCCTCATCACCCAAGCCAGGCACCATGGGCAACGCCATTGAGGAGGAGGCTCCA GAGCTGGACCTGCCGGGGCCCGTGTTTGTGGACGACTTCTACTACGACTACAATTTCATCAATTTCCACG AGGATCTGTCCTACGGGCCCTCTGAGGAGCCCGATCTAGACCTGGCGGGGACAGGGGACCGGACACCCCC ACCACACAGCCATCCTGCTGCGCCCTCCACGGGTAGCCCTGTGCCTGCCACAGAGCCTCCTGCAGCCAAG GAGGAGGGGGTACTGGGACCTTGGTCCCCGAGCCCTTGGCCTAGCCAGGCCGGCCGCTCCCCACCCCCAC CCTCAGAGCAGACCCCTGGGAACCCTTTGATCAATTTCCTGCCTGAGGAAGACACCCCCATAGGGGCCCC AGATCTTGGGCTCCCCAGCCTGTCCTGGCCCAGGGTTTCCACTGATGGCCTGCAGACACCTGCCACCCCT GAGAGCCAAAATGATTTCCCAGTTGGCAAGGACAGCCAGAGCCAGCTGCCCCCTCCATGGCGGGACAGGA CCAATGAGGTTTTCAAGGATGATGAGGAACCCAAGGGCCGCGGAGCACCCCACCTGCCCCCGAGACCCAG CTCCACGCTGCCCCCTTTGTCCCCTGTTGGCAGCACCCACTCCTCTCCTAGTCCTGACGTGGCGGAGCTG TGGACAGGAGGCACAGTGGCCTGGGAGCCAGCTCTGGAGGGTGGCCTGGGGCCTGTGGACAGTGAACTGT GGCCCACTGTTGGGGTGGCTTCTCTCCTTCCTCCTCCCATAGCCCCTCTGCCAGAGATGAAGGTCAGGGA CAGTTCCCTGGAGCCGGGGACTCCCTCCTTCCCAACCCCAGGACCAGGCTCATGGGACCTGCAGACTGTG GCAGTGTGGGGGACCTTCCTCCCCACAACCCTGACTGGCCTCGGGCACATGCCTGAGCCTGCCCTGAACC CAGGACCCAAGGGTCAGCCTGAGTCCCTCAGCCCTGAGGTGCCCCTGAGCTCTAGGCTGCTGTCCACACC AGCTTGGGACAGCCCCGCCAACAGCCACAGAGTCCCTGAGACCCAGCCGCTGGCTCCCAGCCTGGCTGAA GCGGGGCCCCCCGCGGACCCGTTGGTTGTCAGGAACGCCGGCTGGCAAGCGGGAAACTGGAGCGAGTGCT CTACCACCTGTGGCCTGGGTGCGGTCTGGAGGCCGGTGCGCTGTAGCTCCGGCCGGGATGAGGACTGCGC CCCCGCTGGCCGGCCCCAGCCTGCCCGCCGCTGCCACCTGCGGCCCTGTGCCACCTGGCACTCAGGCAAC TGGAGTAAGTGCTCCCGCAGCTGCGGCGGAGGTTCCTCAGTGCGGGACGTGCAGTGTGTGGACACACGGG ACCTCCGGCCACTGCGGCCCTTCCATTGTCAGCCCGGGCCTGCCAAGCCGCCTGCGCACCGGCCCTGCGG GGCCCAGCCCTGCCTCAGCTGGTACACATCTTCCTGGAGGGAGTGCTCCGAGGCCTGTGGCGGTGGTGAG CAGCAGCGTCTAGTGACCTGCCCGGAGCCAGGCCTCTGCGAGGAGGCGCTGAGACCCAACACCACCCGGC CCTGCAACACCCACCCCTGCACGCAGTGGGTGGTGGGGCCCTGGGGCCAGTGCTCAGGCCCCTGTGGTGG TGGTGTCCAGCGGCGCCTGGTCAAGTGTGTCAACACCCAGACAGGGCTGCCCGAGGAAGACAGTGACCAG TGTGGCCACGAGGCCTGGCCTGAGAGCTCCCGGCCGTGTGGCACCGAGGATTGTGAGCCCGTCGAGCCTC CCCGCTGTGAGCGGGACCGCCTGTCCTTCGGGTTCTGCGAGACGCTGCGCCTACTGGGCCGCTGCCAGCT GCCCACCATCCGCACCCAGTGCTGCCGCTCGTGCTCTCCGCCCAGCCACGGCGCCCCCTCCCGAGGCCAT CAGCGGGTTGCCCGCCGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208574 representing NM_014272
Red=Cloning site Green=Tags(s) MPGGPSPRSPAPLLRPLLLLLCALAPGAPGPAPGRATEGRAALDIVHPVRVDAGGSFLSYELWPRALRKR DVSVRRDAPAFYELQYRGRELRFNLTANQHLLAPGFVSETRRRGGLGRAHIRAHTPACHLLGEVQDPELE GGLAAISACDGLKGVFQLSNEDYFIEPLDSAPARPGHAQPHVVYKRQAPERLAQRGDSSAPSTCGVQVYP ELESRRERWEQRQQWRRPRLRRLHQRSVSKEKWVETLVVADAKMVEYHGQPQVESYVLTIMNMVAGLFHD PSIGNPIHITIVRLVLLEDEEEDLKIMHHADNTLKSFCKWQKSINMKGDAHPLHHDTAILLTRKDLCAAM NRPCETLGLSHVAGMCQPHRSCSINEDTGLPLAFTVAHELGHSFGIQHDGSGNDCEPVGKRPFIMSPQLL YDAAPLTWSRCSRQYITRFLDRGWGLCLDDPPAKDIIDFPSVPPGVLYDVSHQCRLQYGAYSAFCEDMDN VCHTLWCSVGTTCHSKLDAAVDGTRCGENKWCLSGECVPVGFRPEAVDGGWSGWSAWSICSRSCGMGVQS AERQCTQPTPKYKGRYCVGERKRFRLCNLQACPAGRPSFRHVQCSHFDAMLYKGQLHTWVPVVNDVNPCE LHCRPANEYFAEKLRDAVVDGTPCYQVRASRDLCINGICKNVGCDFEIDSGAMEDRCGVCHGNGSTCHTV SGTFEEAEGLGYVDVGLIPAGAREIRIQEVAEAANFLALRSEDPEKYFLNGGWTIQWNGDYQVAGTTFTY ARRGNWENLTSPGPTKEPVWIQLLFQESNPGVHYEYTIHREAGGHDEVPPPVFSWHYGPWTKCTVTCGRG VQRQNVYCLERQAGPVDEEHCDPLGRPDDQQRKCSEQPCPARWWAGEWQLCSSSCGPGGLSRRAVLCIRS VGLDEQSALEPPACEHLPRPPTETPCNRHVPCPATWAVGNWSQCSVTCGEGTQRRNVLCTNDTGVPCDEA QQPASEVTCSLPLCRWPLGTLGPEGSGSGSSSHELFNEADFIPHHLAPRPSPASSPKPGTMGNAIEEEAP ELDLPGPVFVDDFYYDYNFINFHEDLSYGPSEEPDLDLAGTGDRTPPPHSHPAAPSTGSPVPATEPPAAK EEGVLGPWSPSPWPSQAGRSPPPPSEQTPGNPLINFLPEEDTPIGAPDLGLPSLSWPRVSTDGLQTPATP ESQNDFPVGKDSQSQLPPPWRDRTNEVFKDDEEPKGRGAPHLPPRPSSTLPPLSPVGSTHSSPSPDVAEL WTGGTVAWEPALEGGLGPVDSELWPTVGVASLLPPPIAPLPEMKVRDSSLEPGTPSFPTPGPGSWDLQTV AVWGTFLPTTLTGLGHMPEPALNPGPKGQPESLSPEVPLSSRLLSTPAWDSPANSHRVPETQPLAPSLAE AGPPADPLVVRNAGWQAGNWSECSTTCGLGAVWRPVRCSSGRDEDCAPAGRPQPARRCHLRPCATWHSGN WSKCSRSCGGGSSVRDVQCVDTRDLRPLRPFHCQPGPAKPPAHRPCGAQPCLSWYTSSWRECSEACGGGE QQRLVTCPEPGLCEEALRPNTTRPCNTHPCTQWVVGPWGQCSGPCGGGVQRRLVKCVNTQTGLPEEDSDQ CGHEAWPESSRPCGTEDCEPVEPPRCERDRLSFGFCETLRLLGRCQLPTIRTQCCRSCSPPSHGAPSRGH QRVARR TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014272 |
| ORF Size | 5058 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014272.3, NP_055087.2 |
| RefSeq Size | 5537 bp |
| RefSeq ORF | 5061 bp |
| Locus ID | 11173 |
| UniProt ID | Q9UKP4 |
| Protein Families | Druggable Genome, Secreted Protein |
| Gene Summary | The protein encoded by this gene is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. Members of this family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs and may regulate vascular smooth muscle cell (VSMC) migration. Mutations in this gene may be associated with susceptibility to coronary artery disease. [provided by RefSeq, Feb 2016] |
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