DUSP8 (NM_004420) Human Tagged ORF Clone
CAT#: RG208337
- TrueORF®
DUSP8 (tGFP-tagged) - Human dual specificity phosphatase 8 (DUSP8)
ORF Plasmid: DDK
"NM_004420" in other vectors (7)
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CNY 4,370.00
CNY 1,000.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C11orf81; HB5; HVH-5; HVH8 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208337 representing NM_004420
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGGGACCGGCTCCCGAGGAAGGTGATGGATGCCAAGAAGCTGGCCAGCCTGCTGCGGGGCGGGC CTGGGGGGCCGCTGGTCATCGACAGCCGCTCCTTCGTGGAGTACAACAGCTGGCATGTGCTCAGCTCCGT CAACATCTGCTGCTCCAAGCTGGTGAAGCGGCGGCTGCAGCAGGGCAAGGTGACCATTGCGGAGCTCATC CAGCCGGCTGCACGCAGCCAGGTGGAGGCTACGGAGCCACAGGACGTGGTGGTCTATGACCAGAGCACGC GGGACGCCAGCGTGCTGGCCGCAGACAGCTTCCTCTCCATCCTGCTGAGCAAGCTGGACGGCTGCTTCGA CAGCGTGGCCATCCTCACTGGGGGCTTCGCCACCTTCTCCTCCTGCTTCCCCGGCCTCTGCGAGGGCAAG CCTGCTGCCCTGCTACCCATGAGCCTCTCCCAGCCCTGCCTGCCTGTGCCCAGCGTGGGCCTCACCCGCA TCCTGCCTCACCTCTACCTGGGCTCGCAGAAGGACGTCCTAAACAAGGATCTGATGACGCAAAATGGAAT AAGCTACGTCCTCAACGCCAGCAACTCCTGCCCCAAGCCTGACTTCATCTGCGAGAGCCGCTTCATGCGG GTCCCCATCAACGACAACTACTGTGAAAAACTGCTGCCCTGGCTGGACAAGTCCATCGAGTTCATCGATA AAGCCAAGCTCTCCAGCTGCCAAGTCATCGTCCACTGTCTGGCTGGCATCTCCCGCTCTGCCACCATCGC CATCGCCTACATCATGAAGACCATGGGCATGTCCTCCGACGACGCCTACAGGTTCGTGAAGGACAGGCGC CCGTCCATCTCGCCCAACTTCAACTTCCTGGGCCAGCTGCTGGAGTACGAGCGCAGCCTGAAGCTGCTGG CCGCCCTGCAGGGCGACCCGGGCACCCCCTCAGGGACGCCGGAGCCTCCGCCCAGTCCTGCCGCCGGGGC CCCGCTGCCACGGCTGCCACCACCTACCTCAGAGAGCGCTGCCACAGGGAATGCGGCTGCCAGGGAGGGC GGCCTGAGCGCGGGCGGGGAGCCCCCCGCGCCCCCCACGCCCCCGGCGACCAGCGCACTGCAGCAGGGCC TGCGCGGCCTGCACCTCTCCTCGGACCGCCTGCAGGACACTAACCGCCTCAAGCGCTCCTTCTCCCTGGA CATCAAGTCTGCCTACGCCCCTAGCAGGCGGCCCGACGGCCCCGGGCCCCCCGACCCCGGCGAGGCCCCG AAGCTCTGCAAGCTGGACAGCCCGTCGGGGGCCGCGCTGGGCCTGTCCTCGCCCAGCCCGGACAGCCCGG ACGCCGCGCCTGAGGCGCGCCCACGGCCCCGCCGGCGGCCCCGGCCCCCCGCCGGCTCCCCCGCGCGCTC CCCCGCGCACAGCCTCGGCCTGAACTTCGGCGATGCGGCCCGGCAGACTCCGCGGCACGGCCTCTCGGCC CTGTCGGCGCCCGGGCTGCCCGGCCCTGGCCAGCCGGCCGGCCCCGGGGCCTGGGCACCGCCGCTCGACT CCCCAGGCACGCCGTCGCCCGACGGGCCCTGGTGCTTCAGCCCCGAGGGCGCACAGGGGGCGGGCGGGGT GCTGTTTGCGCCCTTCGGCCGGGCGGGCGCCCCGGGACCAGGCGGCGGCAGCGACCTGCGGCGGCGGGAG GCAGCGAGGGCTGAGCCCCGGGACGCGCGGACCGGCTGGCCCGAGGAGCCGGCCCCGGAGACGCAGTTCA AGCGCCGCAGCTGCCAGATGGAGTTCGAGGAGGGCATGGTGGAGGGGCGCGCGCGCGGCGAGGAGCTGGC CGCCCTGGGCAAGCAGGCGAGCTTCTCGGGCAGCGTGGAGGTCATCGAGGTGTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208337 representing NM_004420
Red=Cloning site Green=Tags(s) MAGDRLPRKVMDAKKLASLLRGGPGGPLVIDSRSFVEYNSWHVLSSVNICCSKLVKRRLQQGKVTIAELI QPAARSQVEATEPQDVVVYDQSTRDASVLAADSFLSILLSKLDGCFDSVAILTGGFATFSSCFPGLCEGK PAALLPMSLSQPCLPVPSVGLTRILPHLYLGSQKDVLNKDLMTQNGISYVLNASNSCPKPDFICESRFMR VPINDNYCEKLLPWLDKSIEFIDKAKLSSCQVIVHCLAGISRSATIAIAYIMKTMGMSSDDAYRFVKDRR PSISPNFNFLGQLLEYERSLKLLAALQGDPGTPSGTPEPPPSPAAGAPLPRLPPPTSESAATGNAAAREG GLSAGGEPPAPPTPPATSALQQGLRGLHLSSDRLQDTNRLKRSFSLDIKSAYAPSRRPDGPGPPDPGEAP KLCKLDSPSGAALGLSSPSPDSPDAAPEARPRPRRRPRPPAGSPARSPAHSLGLNFGDAARQTPRHGLSA LSAPGLPGPGQPAGPGAWAPPLDSPGTPSPDGPWCFSPEGAQGAGGVLFAPFGRAGAPGPGGGSDLRRRE AARAEPRDARTGWPEEPAPETQFKRRSCQMEFEEGMVEGRARGEELAALGKQASFSGSVEVIEVS TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004420 |
ORF Size | 1875 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004420.1, NP_004411.1 |
RefSeq Size | 2377 bp |
RefSeq ORF | 1878 bp |
Locus ID | 1850 |
UniProt ID | Q13202 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | MAPK signaling pathway |
Gene Summary | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208337 | DUSP8 (Myc-DDK-tagged)-Human dual specificity phosphatase 8 (DUSP8) |
CNY 6,976.00 |
|
RC208337L1 | Lenti ORF clone of Human dual specificity phosphatase 8 (DUSP8), Myc-DDK-tagged |
CNY 9,376.00 |
|
RC208337L2 | Lenti ORF clone of Human dual specificity phosphatase 8 (DUSP8), mGFP tagged |
CNY 5,890.00 |
|
RC208337L3 | Lenti ORF clone of Human dual specificity phosphatase 8 (DUSP8), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC208337L4 | Lenti ORF clone of Human dual specificity phosphatase 8 (DUSP8), mGFP tagged |
CNY 9,376.00 |
|
SC303480 | DUSP8 (untagged)-Human dual specificity phosphatase 8 (DUSP8) |
CNY 10,070.00 |
|
SC320558 | DUSP8 (untagged)-Human dual specificity phosphatase 8 (DUSP8) |
CNY 6,992.00 |