ELL (NM_006532) Human Tagged ORF Clone

CAT#: RG208258

  • TrueORF®

ELL (tGFP-tagged) - Human elongation factor RNA polymerase II (ELL)

ORF Plasmid: DDK tGFP



  "NM_006532" in other vectors (6)

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CNY 8,536.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


ELL mouse monoclonal antibody,clone OTI2H7
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms C19orf17; ELL1; MEN; PPP1R68
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG208258 representing NM_006532
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCTGAAGGAGGATAGGAGCTACGGGCTGTCGTGCGGGCGGGTTAGCGACGGCAGCAAGGTGT
CGGTGTTCCACGTGAAGCTCACCGACAGTGCCCTGAGGGCCTTCGAGAGCTACCGCGCCAGACAGGATTC
TGTTTCACTGAGGCCATCTATCCGATTTCAAGGAAGCCAAGGGCACATCTCCATCCCCCAGCCTGACTGC
CCCGCAGAGGCGCGGACGTTCTCCTTCTACCTCTCCAACATCGGCCGCGACAACCCCCAGGGCAGCTTCG
ACTGCATCCAGCAGTATGTCTCCAGTCATGGGGAAGTTCACCTGGACTGCCTGGGCAGCATACAGGACAA
GATCACGGTGTGTGCCACCGACGACTCCTACCAGAAGGCGCGGCAGAGCATGGCCCAGGCGGAGGAGGAG
ACGCGGAGCCGAAGTGCCATTGTCATCAAGGCTGGAGGCCGCTACCTGGGCAAGAAGGTTCAGTTTCGGA
AACCAGCCCCAGGTGCAACAGACGCGGTGCCCTCCCGGAAGCGGGCAACCCCCATCAACTTGGCGAGTGC
CATCAGGAAGAGTGGTGCCAGTGCCGTGAGTGGGGGCAGCGGGGTGTCCCAGAGGCCCTTCCGTGACCGA
GTGCTGCACCTCCTGGCACTACGGCCCTACCGCAAGGCTGAGCTGCTGCTGCGACTGCAGAAGGACGGCC
TGACGCAGGCGGACAAGGACGCGCTGGATGGCCTCCTCCAGCAGGTGGCCAACATGAGTGCTAAGGACGG
CACGTGTACACTGCAGGACTGCATGTACAAGGATGTGCAGAAGGACTGGCCTGGCTACTCGGAGGGGGAC
CAGCAGCTGCTGAAGCGGGTGCTCGTCCGGAAGCTGTGCCAGCCACAGAGCACTGGCAGCCTCCTTGGAG
ACCCTGCTGCCTCCAGCCCCCCAGGCGAGCGTGGGCGCTCGGCCTCGCCCCCACAGAAGCGGCTGCAGCC
TCCTGATTTCATCGACCCCCTAGCCAACAAGAAACCCCGGATATCGCACTTCACTCAGAGAGCTCAGCCT
GCCGTCAACGGGAAGCTGGGCGTGCCCAATGGCCGTGAGGCCTTGCTGCCCACCCCGGGCCCACCAGCCA
GCACGGACACCCTCAGCTCCAGCACTCACCTGCCCCCGCGGCTGGAGCCCCCGAGGGCCCACGACCCCCT
GGCCGATGTCAGCAATGACCTGGGCCACAGCGGCCGAGACTGTGAGCACGGAGAGGCGGCTGCCCCAGCC
CCCACTGTGCGCCTCGGCCTGCCCCTGCTGACGGACTGTGCCCAGCCCAGCAGGCCACACGGCAGCCCCT
CGCGCAGCAAGCCCAAGAAGAAGTCCAAGAAGCACAAAGACAAGGAGAGGGCGGCTGAGGACAAGCCCCG
GGCCCAGCTTCCAGACTGTGCACCTGCCACCCATGCCACCCCCGGAGCCCCAGCAGACACCCCAGGTTTA
AACGGAACCTGCAGCGTTTCCAGTGTTCCCACGTCCACGTCGGAGACGCCTGACTACTTGCTGAAGTACG
CAGCCATCTCCTCTTCGGAGCAGCGCCAGAGCTACAAGAACGACTTCAATGCCGAGTACAGCGAGTACCG
CGACCTGCACGCCCGCATTGAGCGCATCACGCGGCGGTTCACCCAGCTCGACGCCCAGCTCCGGCAGCTC
TCCCAGGGCTCCGAGGAGTATGAGACTACTCGAGGGCAGATTTTGCAGGAATATCGAAAAATCAAAAAGA
CCAACACCAACTACAGCCAGGAGAAGCACCGCTGCGAGTACCTGCACAGCAAGCTGGCCCACATCAAGAG
GCTCATCGCCGAGTACGACCAGCGGCAGCTGCAGGCTTGGCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208258 representing NM_006532
Red=Cloning site Green=Tags(s)

MAALKEDRSYGLSCGRVSDGSKVSVFHVKLTDSALRAFESYRARQDSVSLRPSIRFQGSQGHISIPQPDC
PAEARTFSFYLSNIGRDNPQGSFDCIQQYVSSHGEVHLDCLGSIQDKITVCATDDSYQKARQSMAQAEEE
TRSRSAIVIKAGGRYLGKKVQFRKPAPGATDAVPSRKRATPINLASAIRKSGASAVSGGSGVSQRPFRDR
VLHLLALRPYRKAELLLRLQKDGLTQADKDALDGLLQQVANMSAKDGTCTLQDCMYKDVQKDWPGYSEGD
QQLLKRVLVRKLCQPQSTGSLLGDPAASSPPGERGRSASPPQKRLQPPDFIDPLANKKPRISHFTQRAQP
AVNGKLGVPNGREALLPTPGPPASTDTLSSSTHLPPRLEPPRAHDPLADVSNDLGHSGRDCEHGEAAAPA
PTVRLGLPLLTDCAQPSRPHGSPSRSKPKKKSKKHKDKERAAEDKPRAQLPDCAPATHATPGAPADTPGL
NGTCSVSSVPTSTSETPDYLLKYAAISSSEQRQSYKNDFNAEYSEYRDLHARIERITRRFTQLDAQLRQL
SQGSEEYETTRGQILQEYRKIKKTNTNYSQEKHRCEYLHSKLAHIKRLIAEYDQRQLQAWP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006532
ORF Size 1863 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006532.4
RefSeq Size 4058 bp
RefSeq ORF 1866 bp
Locus ID 8178
UniProt ID P55199
Protein Families Transcription Factors
Gene Summary Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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