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ELL (NM_006532) Human Tagged Lenti ORF Clone
CAT＃: RC208258L4
- LentiORF®
Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), mGFP tagged
"NM_006532" in other vectors (6)
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CNY 5,890.00
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CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | C19orf17; ELL1; MEN; PPP1R68 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC208258).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006532 |
| ORF Size | 1863 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006532.2 |
| RefSeq Size | 4077 bp |
| RefSeq ORF | 1866 bp |
| Locus ID | 8178 |
| UniProt ID | P55199 |
| Protein Families | Transcription Factors |
| MW | 68.3 kDa |
| Gene Summary | Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780). Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780). ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208258 | ELL (Myc-DDK-tagged)-Human elongation factor RNA polymerase II (ELL) |
CNY 6,936.00 |
|
| RC208258L1 | Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), Myc-DDK-tagged |
CNY 9,336.00 |
|
| RC208258L2 | Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), mGFP tagged |
CNY 9,336.00 |
|
| RC208258L3 | Lenti ORF clone of Human elongation factor RNA polymerase II (ELL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG208258 | ELL (tGFP-tagged) - Human elongation factor RNA polymerase II (ELL) |
CNY 8,536.00 |
|
| SC111065 | ELL (untagged)-Human elongation factor RNA polymerase II (ELL) |
CNY 6,944.00 |
