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PPP1R3D (NM_006242) Human Tagged ORF Clone
CAT＃: RG207987
- TrueORF®
PPP1R3D (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D)
ORF Plasmid: DDK
"NM_006242" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PPP1R6 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207987 representing NM_006242
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGAGGCCCGAGCTCCGCGGTCCTGCCTAGCGCCCTGGGATCCCGGAAGCTCGGCCCCCGGAGCC TCAGCTGCCTGTCGGACCTGGACGGCGGCGTGGCCCTGGAGCCGCGGGCCTGTAGGCCCCCTGGGAGCCC GGGCCGCGCGCCGCCGCCAACGCCAGCGCCGTCGGGCTGCGACCCCCGCCTGCGGCCCATCATCCTGCGG CGGGCGCGCTCACTGCCCAGCTCCCCCGAGCGCCGCCAGAAGGCCGCGGGCGCGCCGGGCGCTGCGTGTC GGCCGGGCTGCAGCCAGAAGCTCCGCGTGCGCTTCGCCGACGCCCTGGGCTTGGAGCTGGCACAGGTCAA GGTGTTCAACGCGGGAGACGACCCGTCCGTGCCGCTGCACGTGCTGTCGCGGCTCGCAATCAACTCGGAC CTGTGCTGCAGCAGCCAGGACCTGGAGTTCACCCTGCATTGCCTGGTGCCCGATTTCCCGCCGCCCGTCG AGGCCGCCGACTTTGGCGAGCGCCTGCAGCGGCAGCTCGTGTGCCTGGAGCGTGTCACTTGCTCGGACCT TGGCATCAGCGGTACGGTGCGCGTGTGCAACGTGGCCTTCGAGAAGCAGGTGGCTGTGCGCTACACTTTC TCGGGCTGGCGCAGTACCCACGAGGCGGTGGCGCGGTGGCGCGGGCCCGCAGGCCCCGAGGGCACGGAGG ACGTTTTCACCTTCGGCTTTCCAGTACCGCCCTTCCTGCTGGAGCTCGGCTCCCGCGTGCACTTCGCGGT GCGCTACCAAGTGGCGGGTGCCGAGTACTGGGACAACAACGACCACCGAGACTACAGCCTCACATGTCGC AACCACGCGCTGCACATGCCTCGCGGGGAGTGCGAAGAGAGCTGGATCCACTTCATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207987 representing NM_006242
Red=Cloning site Green=Tags(s) MSRGPSSAVLPSALGSRKLGPRSLSCLSDLDGGVALEPRACRPPGSPGRAPPPTPAPSGCDPRLRPIILR RARSLPSSPERRQKAAGAPGAACRPGCSQKLRVRFADALGLELAQVKVFNAGDDPSVPLHVLSRLAINSD LCCSSQDLEFTLHCLVPDFPPPVEAADFGERLQRQLVCLERVTCSDLGISGTVRVCNVAFEKQVAVRYTF SGWRSTHEAVARWRGPAGPEGTEDVFTFGFPVPPFLLELGSRVHFAVRYQVAGAEYWDNNDHRDYSLTCR NHALHMPRGECEESWIHFI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006242 |
| ORF Size | 897 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006242.4 |
| RefSeq Size | 3481 bp |
| RefSeq ORF | 900 bp |
| Locus ID | 5509 |
| UniProt ID | O95685 |
| Domains | CBM_21 |
| Protein Families | Druggable Genome, Phosphatase |
| Protein Pathways | Insulin signaling pathway |
| Gene Summary | Phosphorylation of serine and threonine residues in proteins is a crucial step in the regulation of many cellular functions ranging from hormonal regulation to cell division and even short-term memory. The level of phosphorylation is controlled by the opposing actions of protein kinases and protein phosphatases. Protein phosphatase 1 (PP1) is 1 of 4 major serine/threonine-specific protein phosphatases which have been identified in eukaryotic cells. PP1 associates with various regulatory subunits that dictate its subcellular localization and modulate its substrate specificity. Several subunits that target PP1 to glycogen have been identified. This gene encodes a glycogen-targeting subunit of PP1. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207987 | PPP1R3D (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) |
CNY 3,990.00 |
|
| RC207987L3 | Lenti-ORF clone of PPP1R3D (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) |
CNY 5,890.00 |
|
| RC207987L4 | Lenti-ORF clone of PPP1R3D (mGFP-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) |
CNY 5,890.00 |
|
| SC125491 | PPP1R3D (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3D (PPP1R3D) |
CNY 2,400.00 |
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