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RNF149 (NM_173647) Human Tagged ORF Clone
CAT＃: RG207979
- TrueORF®
RNF149 (tGFP-tagged) - Human ring finger protein 149 (RNF149)
ORF Plasmid: DDK
"NM_173647" in other vectors (6)
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CNY 5,256.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | DNAPTP2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207979 representing NM_173647
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTGGCGGCGGCGCGAAGCCAGCGTCGGGGCTCGCGGCGTGTTGGCTCTGGCGTTGCTCGCCCTGG CCCTGTGCGTGCCCGGGGCCCGGGGCCGGGCTCTCGAGTGGTTCTCGGCCGTGGTAAACATCGAGTACGT GGACCCGCAGACCAACCTGACGGTGTGGAGCGTCTCGGAGAGTGGCCGCTTCGGCGACAGCTCGCCCAAG GAGGGCGCGCATGGCCTGGTGGGCGTCCCGTGGGCGCCCGGCGGAGACCTCGAGGGCTGCGCGCCCGACA CGCGCTTCTTCGTGCCCGAGCCCGGCGGCCGAGGGGCCGCGCCCTGGGTCGCCCTGGTGGCTCGTGGGGG CTGCACCTTCAAGGACAAGGTGCTGGTGGCGGCGCGGAGGAACGCCTCGGCCGTCGTCCTCTACAATGAG GAGCGCTACGGGAACATCACCTTGCCCATGTCTCACGCGGGAACAGGAAATATAGTGGTCATTATGATTA GCTATCCAAAAGGAAGAGAAATTTTGGAGCTGGTGCAAAAAGGAATTCCAGTAACGATGACCATAGGGGT TGGCACCCGGCATGTACAGGAGTTCATCAGCGGTCAGTCTGTGGTGTTTGTGGCCATTGCCTTCATCACC ATGATGATTATCTCGTTAGCCTGGCTAATATTTTACTATATACAGCGTTTCCTATATACTGGCTCTCAGA TTGGAAGTCAGAGCCATAGAAAAGAAACTAAGAAAGTTATTGGCCAGCTTCTACTTCATACTGTAAAGCA TGGAGAAAAGGGAATTGATGTTGATGCTGAAAATTGTGCAGTGTGTATTGAAAATTTCAAAGTAAAGGAT ATTATTAGAATTCTGCCATGCAAGCATATTTTTCATAGAATATGCATTGACCCATGGCTTTTGGATCACC GAACATGTCCAATGTGTAAACTTGATGTCATCAAAGCCCTAGGATATTGGGGAGAGCCTGGGGATGTACA GGAGATGCCTGCTCCAGAATCTCCTCCTGGAAGGGATCCAGCTGCAAATTTGAGTCTAGCTTTACCAGAT GATGACGGAAGTGATGAGAGCAGTCCACCATCAGCCTCCCCTGCTGAATCTGAGCCACAGTGTGATCCCA GCTTTAAAGGAGATGCAGGAGAAAATACGGCATTGCTAGAAGCCGGCAGGAGTGACTCTCGGCATGGAGG ACCCATCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207979 representing NM_173647
Red=Cloning site Green=Tags(s) MAWRRREASVGARGVLALALLALALCVPGARGRALEWFSAVVNIEYVDPQTNLTVWSVSESGRFGDSSPK EGAHGLVGVPWAPGGDLEGCAPDTRFFVPEPGGRGAAPWVALVARGGCTFKDKVLVAARRNASAVVLYNE ERYGNITLPMSHAGTGNIVVIMISYPKGREILELVQKGIPVTMTIGVGTRHVQEFISGQSVVFVAIAFIT MMIISLAWLIFYYIQRFLYTGSQIGSQSHRKETKKVIGQLLLHTVKHGEKGIDVDAENCAVCIENFKVKD IIRILPCKHIFHRICIDPWLLDHRTCPMCKLDVIKALGYWGEPGDVQEMPAPESPPGRDPAANLSLALPD DDGSDESSPPSASPAESEPQCDPSFKGDAGENTALLEAGRSDSRHGGPIS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173647 |
| ORF Size | 1200 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173647.2, NP_775918.1 |
| RefSeq Size | 2452 bp |
| RefSeq ORF | 1203 bp |
| Locus ID | 284996 |
| UniProt ID | Q8NC42 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207979 | RNF149 (Myc-DDK-tagged)-Human ring finger protein 149 (RNF149) |
CNY 3,656.00 |
|
| RC207979L1 | Lenti ORF clone of Human ring finger protein 149 (RNF149), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC207979L2 | Lenti ORF clone of Human ring finger protein 149 (RNF149), mGFP tagged |
CNY 5,890.00 |
|
| RC207979L3 | Lenti ORF clone of Human ring finger protein 149 (RNF149), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207979L4 | Lenti ORF clone of Human ring finger protein 149 (RNF149), mGFP tagged |
CNY 5,890.00 |
|
| SC111569 | RNF149 (untagged)-Human ring finger protein 149 (RNF149) |
CNY 3,656.00 |
