PCDH1 (NM_002587) Human Tagged ORF Clone
CAT#: RG207717
- TrueORF®
PCDH1 (tGFP-tagged) - Human protocadherin 1 (PCDH1), transcript variant 1
ORF Plasmid: DDK
"NM_002587" in other vectors (4)
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CNY 11,590.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PC42; PCDH42 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG207717 representing NM_002587
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCGGGGCGGGCGGCCGGCGCTGCCCGGAGGCGGCCCTCCTGATTCTGGGGCCTCCCAGGATGG AGCACCTGAGGCACAGCCCAGGCCCTGGGGGGCAACGGCTACTGCTGCCCTCCATGCTGCTAGCACTGCT GCTCCTGCTGGCTCCATCCCCAGGCCACGCCACTCGGGTAGTGTACAAGGTGCCGGAGGAACAGCCACCC AACACCCTCATTGGGAGCCTCGCAGCCGACTATGGTTTTCCAGATGTGGGGCACCTGTACAAGCTAGAGG TGGGTGCCCCGTACCTTCGCGTGGATGGCAAGACAGGTGACATTTTCACCACCGAGACCTCCATCGACCG TGAGGGGCTCCGTGAATGCCAGAACCAGCTCCCTGGTGATCCCTGCATCCTGGAGTTTGAGGTATCTATC ACAGACCTCGTGCAGAATGGCAGCCCCCGGCTGCTAGAGGGCCAGATAGAAGTACAAGACATCAATGACA ACACACCCAACTTCGCCTCACCAGTCATCACTCTGGCCATCCCTGAGAACACCAACATCGGCTCACTCTT CCCCATCCCGCTGGCTTCAGACCGTGATGCTGGTCCCAACGGTGTGGCATCCTATGAGCTGCAGGCTGGG CCTGAGGCCCAGGAGCTATTTGGGCTGCAGGTGGCAGAGGACCAGGAGGAGAAGCAACCACAGCTCATTG TGATGGGCAACCTGGACCGTGAGCGCTGGGACTCCTATGACCTCACCATCAAGGTGCAGGATGGCGGCAG CCCCCCACGCGCCAGCAGTGCCCTGCTGCGTGTCACCGTGCTTGACACCAATGACAACGCCCCCAAGTTT GAGCGGCCCTCCTATGAGGCCGAACTATCTGAGAATAGCCCCATAGGCCACTCGGTCATCCAGGTGAAGG CCAATGACTCAGACCAAGGTGCCAATGCAGAAATCGAATACACATTCCACCAGGCGCCCGAAGTTGTGAG GCGTCTTCTTCGACTGGACAGGAACACTGGACTTATCACTGTTCAGGGCCCGGTGGACCGTGAGGACCTA AGCACCCTGCGCTTCTCAGTGCTTGCTAAGGACCGAGGCACCAACCCCAAGAGTGCCCGTGCCCAGGTGG TTGTGACCGTGAAGGACATGAATGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCA TCAAGATGGGATGGCTAACATCTCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCT GACCGAGATGAGGGAGAGAATGCAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCC AGGCCAGTGAGACAGGCAGTGACAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGA GAAGGTCAAAGACTACACCATTGAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAAC TCCCTCAAGGTGCAGGTGGTGGACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCG CCTTCCCGGAAAACAACAAGCCTGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTC TAATGCTGAGCTGGTTTACTCTCTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAG ACTGGAGAGATCCAGGTGAAGACATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGG CAGCTGACCGGGGCAGTCCTAGCCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGA CAATGACCCCAAATTTATGCTGAGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCA GTGGGCATGGTGACTGTCATTGATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGG ACAACGGTGACTTTGTTATCCAGAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCA ACAAAGCACCTACACCTTCCAGCTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGT GTCACCATCAATGTGCTGGACGAGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACA AGCTGCTGACCCCCCAGACACGTCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGG TGTCAATGCCGAGCTGATCTACAGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACAT TCAGGTGCCATCACCCTGGAGAAGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGG TCAGTGACCGCGGCAAGCCCCCACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGC CAACCGCACGCTGCTGGAGACCCTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGG GATCCAGAATATGAGCGCTCCAAGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCG TGGCCTTGCTCATCGCCCTGGCGGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCA GGCTGGTAAGAAGGAGACCAAGGACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAA AGCAAAGGCAAGAAGAGCAAGTCCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGC AGAAGTCCCTCAAGTTCAACCTGATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAA CTACCCACCAGGCAGCCCTGACCTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTG CAGCCCCAGTCACCCTCAGCCTCCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCG TGGGCACCGGGGACACCACGTCCACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCC CAAATACCCCAGCAAGCAGGTAGGCCAGCCCTTTCAGCTCAGCACACCCCAGCCCCTACCCCACCCCTAC CACGGAGCCATCTGGACCGAGGTGTGGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207717 representing NM_002587
Red=Cloning site Green=Tags(s) MDSGAGGRRCPEAALLILGPPRMEHLRHSPGPGGQRLLLPSMLLALLLLLAPSPGHATRVVYKVPEEQPP NTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQLPGDPCILEFEVSI TDLVQNGSPRLLEGQIEVQDINDNTPNFASPVITLAIPENTNIGSLFPIPLASDRDAGPNGVASYELQAG PEAQELFGLQVAEDQEEKQPQLIVMGNLDRERWDSYDLTIKVQDGGSPPRASSALLRVTVLDTNDNAPKF ERPSYEAELSENSPIGHSVIQVKANDSDQGANAEIEYTFHQAPEVVRRLLRLDRNTGLITVQGPVDREDL STLRFSVLAKDRGTNPKSARAQVVVTVKDMNDNAPTIEIRGIGLVTHQDGMANISEDVAEETAVALVQVS DRDEGENAAVTCVVAGDVPFQLRQASETGSDSKKKYFLQTTTPLDYEKVKDYTIEIVAVDSGNPPLSSTN SLKVQVVDVNDNAPVFTQSVTEVAFPENNKPGEVIAEITASDADSGSNAELVYSLEPEPAAKGLFTISPE TGEIQVKTSLDREQRESYELKVVAADRGSPSLQGTATVLVNVLDCNDNDPKFMLSGYNFSVMENMPALSP VGMVTVIDGDKGENAQVQLSVEQDNGDFVIQNGTGTILSSLSFDREQQSTYTFQLKAVDGGVPPRSAYVG VTINVLDENDNAPYITAPSNTSHKLLTPQTRLGETVSQVAAEDFDSGVNAELIYSIAGGNPYGLFQIGSH SGAITLEKEIERRHHGLHRLVVKVSDRGKPPRYGTALVHLYVNETLANRTLLETLLGHSLDTPLDIDIAG DPEYERSKQRGNILFGVVAGVVAVALLIALAVLVRYCRQREAKSGYQAGKKETKDLYAPKPSGKASKGNK SKGKKSKSPKPVKPVEDEDEAGLQKSLKFNLMSDAPGDSPRIHLPLNYPPGSPDLGRHYRSNSPLPSIQL QPQSPSASKKHQVVQDLPPANTFVGTGDTTSTGSEQYSDYSYRTNPPKYPSKQVGQPFQLSTPQPLPHPY HGAIWTEVWE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002587 |
ORF Size | 3180 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002587.3, NP_002578.2 |
RefSeq Size | 3851 bp |
RefSeq ORF | 3183 bp |
Locus ID | 5097 |
UniProt ID | Q08174 |
Domains | CA |
Protein Families | Transcription Factors, Transmembrane |
Gene Summary | This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207717 | PCDH1 (Myc-DDK-tagged)-Human protocadherin 1 (PCDH1), transcript variant 1 |
CNY 11,072.00 |
|
RC207717L3 | Lenti ORF clone of Human protocadherin 1 (PCDH1), transcript variant 1, Myc-DDK-tagged |
CNY 12,450.00 |
|
RC207717L4 | Lenti ORF clone of Human protocadherin 1 (PCDH1), transcript variant 1, mGFP tagged |
CNY 12,450.00 |
|
SC109553 | PCDH1 (untagged)-Human protocadherin 1 (PCDH1), transcript variant 1 |
CNY 11,080.00 |