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Karyopherin beta 3 (IPO5) (NM_002271) Human Tagged ORF Clone
CAT＃: RG207221
- TrueORF®
IPO5 (tGFP-tagged) - Human importin 5 (IPO5)
ORF Plasmid: DDK
"NM_002271" in other vectors (7)
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CNY 12,260.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | IMB3; imp5; KPNB3; Pse1; RANBP5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207221 representing NM_002271
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGAGGATCAAGTTGGAAAACTAGAAGCAACAGAAAACACAATAAGCGCAATGGCGGCGGCCGCGG CGGAGCAGCAACAGTTCTACCTGCTCCTGGGAAACCTGCTCAGCCCCGACAATGTGGTCCGGAAACAGGC AGAGGAAACCTATGAGAATATCCCAGGCCAGTCAAAGATCACATTCCTCTTACAAGCCATCAGAAATACA ACAGCTGCTGAAGAGGCTAGACAAATGGCCGCCGTTCTCCTAAGACGTCTCTTGTCCTCTGCATTTGATG AAGTCTATCCAGCACTTCCCTCTGATGTTCAGACTGCCATCAAGAGTGAGCTACTCATGATTATTCAGAT GGAAACACAATCTAGCATGAGGAAAAAAGTTTGTGATATTGCGGCAGAACTGGCCAGGAATTTAATAGAT GAGGATGGCAATAACCAGTGGCCCGAAGGTTTGAAGTTCCTTTTTGATTCAGTCAGCTCTCAAAATGTGG GACTGCGGGAAGCTGCCCTTCACATTTTCTGGAACTTTCCTGGAATTTTTGGGAACCAGCAACAACACTA TTTAGATGTCATCAAACGAATGTTAGTTCAGTGTATGCAAGATCAGGAACACCCGTCGATCAGGACGTTA TCTGCTAGAGCTACAGCTGCATTTATACTTGCAAATGAGCATAATGTTGCTCTGTTCAAACATTTTGCAG ACTTGCTACCGGGATTCCTACAGGCGGTAAATGACTCGTGCTACCAGAATGATGATTCTGTCCTAAAATC CCTCGTTGAGATTGCAGATACTGTTCCAAAGTATTTGCGTCCTCACTTGGAAGCAACTCTACAGCTAAGT CTAAAGTTGTGTGGAGACACTAGCCTCAACAATATGCAACGCCAGCTTGCCCTTGAAGTGATCGTCACCC TCTCTGAGACTGCAGCTGCTATGTTAAGAAAACATACCAATATTGTTGCACAGACTATTCCTCAGATGTT AGCAATGATGGTTGATTTGGAAGAAGATGAGGACTGGGCAAATGCAGATGAACTAGAAGATGATGATTTT GACAGCAATGCAGTTGCAGGCGAGAGTGCTCTAGATCGAATGGCTTGCGGACTTGGTGGAAAGCTCGTTC TGCCGATGATCAAGGAACACATTATGCAAATGCTTCAAAATCCTGACTGGAAATACCGGCATGCAGGATT GATGGCCTTATCTGCCATTGGTGAAGGGTGCCACCAGCAAATGGAAGGAATTCTAAATGAGATCGTAAAT TTTGTTTTACTTTTTCTCCAGGATCCTCATCCAAGAGTAAGGTATGCAGCCTGTAATGCCGTGGGACAGA TGGCTACAGATTTTGCACCTGGTTTCCAAAAGAAATTTCATGAGAAGGTGATTGCAGCTCTGCTGCAGAC CATGGAAGACCAAGGCAATCAACGTGTGCAGGCCCATGCAGCTGCTGCCCTCATTAACTTTACTGAAGAC TGTCCCAAGTCACTACTTATTCCATACTTGGATAATTTGGTGAAACATCTGCATTCCATTATGGTACTGA AGCTTCAAGAGCTGATTCAGAAAGGCACCAAGTTAGTTTTGGAACAAGTTGTGACATCCATTGCATCAGT TGCCGATACTGCAGAAGAAAAATTTGTCCCCTACTATGATTTATTTATGCCATCACGGAAGCACATCGTT GAGAATGCGGTTCAAAAAGAACTGAGACTTCTGAGAGGAAAAACTATTGAATGCATTAGCCTCATTGGTC TGGCTGTTGGGAAGGAAAAATTCATGCAGGATGCATCAGATGTGATGCAGCTTTTGTTAAAGACCCAGAC AGACTTCAATGATATGGAAGATGATGATCCTCAGATCTCTTACATGATCTCAGCATGGGCCAGAATGTGC AAAATCCTTGGAAAAGAATTTCAGCAATACCTTCCAGTGGTTATGGGGCCTTTAATGAAGACGGCTTCAA TTAAGCCCGAAGTAGCCCTTTTAGATACCCAAGACATGGAGAATATGAGTGATGATGATGGTTGGGAATT TGTGAACCTTGGAGATCAGCAAAGCTTTGGTATTAAAACTGCAGGACTAGAAGAAAAATCAACTGCTTGC CAGATGTTGGTTTGCTATGCTAAGGAGTTAAAGGAAGGCTTTGTGGAGTACACCGAACAGGTTGTCAAAC TGATGGTCCCTTTACTGAAATTTTATTTCCACGATGGTGTTCGAGTGGCAGCAGCGGAATCCATGCCTCT TCTCCTGGAGTGTGCAAGAGTCCGTGGTCCTGAGTACCTCACACAGATGTGGCATTTTATGTGTGATGCT CTAATTAAGGCCATTGGTACAGAACCAGATTCAGACGTCCTCTCAGAAATAATGCATTCTTTTGCAAAGT GCATTGAAGTAATGGGAGATGGATGCCTTAATAATGAACACTTTGAAGAACTGGGAGGTATATTGAAAGC AAAGCTTGAAGAACATTTTAAAAATCAAGAATTACGACAAGTTAAAAGACAAGATGAAGACTATGATGAA CAGGTCGAAGAGTCACTACAAGATGAGGATGATAATGATGTTTATATTCTGACCAAAGTGTCAGATATTT TACACTCAATATTCAGTAGCTACAAAGAAAAGGTGTTACCATGGTTTGAACAGCTGCTTCCATTAATTGT CAACCTCATTTGTCCACATAGACCATGGCCAGACAGACAATGGGGATTATGCATCTTTGATGATGTCATA GAACACTGTAGTCCAGCCTCATTTAAATACGCAGAATATTTCTTAAGACCAATGCTCCAATATGTATGTG ACAACAGCCCAGAAGTCAGGCAAGCAGCTGCATATGGCCTGGGAGTCATGGCACAGTACGGTGGAGATAA TTATCGCCCTTTTTGTACAGAAGCACTTCCCCTGCTGGTAAGAGTTATTCAGTCTGCGGATTCTAAGACC AAAGAAAATGTCAATGCTACAGAGAACTGCATCTCAGCAGTAGGGAAAATCATGAAGTTCAAGCCTGACT GTGTAAACGTTGAAGAGGTCCTTCCACACTGGTTGTCTTGGCTTCCACTACATGAAGATAAAGAAGAAGC TGTTCAGACTTTCAATTATCTGTGTGACCTGATTGAAAGTAATCATCCAATTGTTCTTGGCCCAAACAAT ACCAATCTGCCCAAAATATTTAGTATAATTGCGGAAGGAGAAATGCACGAGGCAATTAAACATGAAGATC CTTGTGCCAAACGTCTGGCCAATGTCGTTCGCCAAGTACAGACTTCTGGAGGACTGTGGACTGAGTGCAT AGCACAGCTCAGTCCTGAGCAGCAGGCCGCCATTCAGGAGCTCCTGAACTCTGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207221 representing NM_002271
Red=Cloning site Green=Tags(s) MPEDQVGKLEATENTISAMAAAAAEQQQFYLLLGNLLSPDNVVRKQAEETYENIPGQSKITFLLQAIRNT TAAEEARQMAAVLLRRLLSSAFDEVYPALPSDVQTAIKSELLMIIQMETQSSMRKKVCDIAAELARNLID EDGNNQWPEGLKFLFDSVSSQNVGLREAALHIFWNFPGIFGNQQQHYLDVIKRMLVQCMQDQEHPSIRTL SARATAAFILANEHNVALFKHFADLLPGFLQAVNDSCYQNDDSVLKSLVEIADTVPKYLRPHLEATLQLS LKLCGDTSLNNMQRQLALEVIVTLSETAAAMLRKHTNIVAQTIPQMLAMMVDLEEDEDWANADELEDDDF DSNAVAGESALDRMACGLGGKLVLPMIKEHIMQMLQNPDWKYRHAGLMALSAIGEGCHQQMEGILNEIVN FVLLFLQDPHPRVRYAACNAVGQMATDFAPGFQKKFHEKVIAALLQTMEDQGNQRVQAHAAAALINFTED CPKSLLIPYLDNLVKHLHSIMVLKLQELIQKGTKLVLEQVVTSIASVADTAEEKFVPYYDLFMPSRKHIV ENAVQKELRLLRGKTIECISLIGLAVGKEKFMQDASDVMQLLLKTQTDFNDMEDDDPQISYMISAWARMC KILGKEFQQYLPVVMGPLMKTASIKPEVALLDTQDMENMSDDDGWEFVNLGDQQSFGIKTAGLEEKSTAC QMLVCYAKELKEGFVEYTEQVVKLMVPLLKFYFHDGVRVAAAESMPLLLECARVRGPEYLTQMWHFMCDA LIKAIGTEPDSDVLSEIMHSFAKCIEVMGDGCLNNEHFEELGGILKAKLEEHFKNQELRQVKRQDEDYDE QVEESLQDEDDNDVYILTKVSDILHSIFSSYKEKVLPWFEQLLPLIVNLICPHRPWPDRQWGLCIFDDVI EHCSPASFKYAEYFLRPMLQYVCDNSPEVRQAAAYGLGVMAQYGGDNYRPFCTEALPLLVRVIQSADSKT KENVNATENCISAVGKIMKFKPDCVNVEEVLPHWLSWLPLHEDKEEAVQTFNYLCDLIESNHPIVLGPNN TNLPKIFSIIAEGEMHEAIKHEDPCAKRLANVVRQVQTSGGLWTECIAQLSPEQQAAIQELLNSA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002271 |
| ORF Size | 3345 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002271.4, NP_002262.3 |
| RefSeq Size | 6019 bp |
| RefSeq ORF | 3294 bp |
| Locus ID | 3843 |
| UniProt ID | O00410 |
| Protein Families | Druggable Genome |
| Gene Summary | Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207221 | IPO5 (Myc-DDK-tagged)-Human importin 5 (IPO5) |
CNY 7,768.00 |
|
| RC207221L1 | Lenti-ORF clone of IPO5 (Myc-DDK-tagged)-Human importin 5 (IPO5) |
CNY 10,168.00 |
|
| RC207221L2 | Lenti-ORF clone of IPO5 (mGFP-tagged)-Human importin 5 (IPO5) |
CNY 13,020.00 |
|
| RC207221L3 | Lenti-ORF clone of IPO5 (Myc-DDK-tagged)-Human importin 5 (IPO5) |
CNY 13,020.00 |
|
| RC207221L4 | Lenti-ORF clone of IPO5 (mGFP-tagged)-Human importin 5 (IPO5) |
CNY 13,020.00 |
|
| SC118726 | IPO5 (untagged)-Human importin 5 (IPO5) |
CNY 7,648.00 |
|
| SC318500 | IPO5 (untagged)-Human importin 5 (IPO5) |
CNY 10,450.00 |
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