United States
Germany
Japan
United Kingdom
China
CIAO3 (NM_022493) Human Tagged ORF Clone
CAT＃: RG206978
- TrueORF®
NARFL (tGFP-tagged) - Human nuclear prelamin A recognition factor-like (NARFL)
ORF Plasmid: DDK
"NM_022493" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | HPRN; IOP1; LET1L; NAR1; NARFL; PRN |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206978 representing NM_022493
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGCCCTTCAGCGGGGCGCTGCAGCTGACGGACCTGGATGACTTCATCGGGCCGTCTCAGGAGT GCATCAAGCCTGTCAAAGTGGAAAAAAGGGCGGGAAGTGGCGTGGCCAAGATTCGCATTGAAGATGACGG GAGCTACTTCCAAATTAACCAAGACGGCGGGACCCGGAGGCTGGAGAAGGCCAAGGTCTCGCTAAACGAC TGCCTGGCGTGCAGCGGCTGCATCACCTCCGCAGAGACCGTGCTTATCACCCAGCAGAGCCACGAGGAGC TGAAGAAGGTTCTAGATGCTAACAAGATGGCGGCACCCAGTCAGCAGAGGCTGGTTGTAGTTTCGGTCTC ACCACAGTCTAGAGCATCGCTGGCTGCACGGTTTCAGCTGAATCCTACAGATACTGCCAGGAAATTAACC TCATTCTTTAAAAAAATAGGGGTGCACTTCGTCTTCGACACCGCCTTCTCAAGGCACTTCAGCCTCCTGG AGAGCCAGCGAGAGTTTGTGCGGCGATTCCGAGGACAGGCCGACTGCAGACAGGCGCTGCCCCTGCTGGC CTCTGCCTGCCCAGGCTGGATCTGCTATGCCGAGAAGACTCACGGCAGCTTCATCCTCCCCCACATCAGC ACCGCCCGGTCCCCGCAGCAGGTCATGGGCTCCCTGGTCAAGGACTTCTTCGCCCAGCAGCAGCACTTGA CCCCTGACAAGATCTACCACGTCACAGTGATGCCCTGCTATGACAAAAAGCTGGAAGCCTCCAGACCCGA CTTTTTCAACCAGGAGCACCAGACACGGGATGTGGACTGTGTCCTCACAACAGGAGAAGTTTTCAGGTTG CTGGAGGAAGAGGGCGTCTCCCTCCCCGACCTGGAACCAGCCCCTCTGGACAGCCTGTGCAGCGGTGCCT CTGCAGAGGAGCCCACCAGCCATCGGGGAGGGGGCTCGGGGGGCTACCTGGAGCACGTGTTCCGGCACGC GGCCCGAGAGCTCTTTGGAATCCATGTGGCTGAGGTTACCTACAAACCCCTGAGGAACAAAGACTTCCAG GAGGTGACACTGGAGAAGGAGGGCCAGGTGCTGCTGCACTTCGCAATGGCGTACGGCTTCCGCAACATCC AGAACCTGGTGCAGAGGCTCAAACGAGGGCGCTGCCCCTACCACTACGTGGAGGTCATGGCCTGCCCCTC AGGCTGCCTGAACGGCGGGGGCCAGCTCCAGGCCCCAGACAGGCCCAGCAGAGAGCTCCTCCAGCACGTG GAGAGACTGTACGGCATGGTCCGGGCTGAGGCGCCCGAGGACGCGCCTGGGGTTCAGGAGCTGTACACAC ACTGGCTGCAGGGCACGGACTCGGAGTGTGCAGGTCGCTTGCTGCATACGCAGTACCACGCCGTGGAGAA GGCCAGCACTGGCCTGGGCATCCGGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206978 representing NM_022493
Red=Cloning site Green=Tags(s) MASPFSGALQLTDLDDFIGPSQECIKPVKVEKRAGSGVAKIRIEDDGSYFQINQDGGTRRLEKAKVSLND CLACSGCITSAETVLITQQSHEELKKVLDANKMAAPSQQRLVVVSVSPQSRASLAARFQLNPTDTARKLT SFFKKIGVHFVFDTAFSRHFSLLESQREFVRRFRGQADCRQALPLLASACPGWICYAEKTHGSFILPHIS TARSPQQVMGSLVKDFFAQQQHLTPDKIYHVTVMPCYDKKLEASRPDFFNQEHQTRDVDCVLTTGEVFRL LEEEGVSLPDLEPAPLDSLCSGASAEEPTSHRGGGSGGYLEHVFRHAARELFGIHVAEVTYKPLRNKDFQ EVTLEKEGQVLLHFAMAYGFRNIQNLVQRLKRGRCPYHYVEVMACPSGCLNGGGQLQAPDRPSRELLQHV ERLYGMVRAEAPEDAPGVQELYTHWLQGTDSECAGRLLHTQYHAVEKASTGLGIRW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022493 |
| ORF Size | 1428 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022493.3 |
| RefSeq Size | 2109 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 64428 |
| UniProt ID | Q9H6Q4 |
| Gene Summary | Component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206978 | NARFL (Myc-DDK-tagged)-Human nuclear prelamin A recognition factor-like (NARFL) |
CNY 3,656.00 |
|
| RC206978L1 | Lenti ORF clone of Human nuclear prelamin A recognition factor-like (NARFL), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206978L2 | Lenti ORF clone of Human nuclear prelamin A recognition factor-like (NARFL), mGFP tagged |
CNY 5,890.00 |
|
| RC206978L3 | Lenti ORF clone of Human nuclear prelamin A recognition factor-like (NARFL), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206978L4 | Lenti ORF clone of Human nuclear prelamin A recognition factor-like (NARFL), mGFP tagged |
CNY 5,890.00 |
|
| SC112504 | NARFL (untagged)-Human nuclear prelamin A recognition factor-like (NARFL) |
CNY 3,656.00 |
|
| SC323948 | NARFL (untagged)-Human nuclear prelamin A recognition factor-like (NARFL) |
CNY 3,656.00 |
