United States
Germany
Japan
United Kingdom
China
AF9 (MLLT3) (NM_004529) Human Tagged ORF Clone
CAT＃: RG206283
- TrueORF®
MLLT3 (tGFP-tagged) - Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3)
ORF Plasmid: DDK
"NM_004529" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,832.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AF9; YEATS3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206283 representing NM_004529
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTAGCTCGTGTGCCGTGCAGGTGAAGCTGGAGCTGGGGCACCGCGCCCAGGTGAGGAAAAAACCCA CCGTGGAGGGCTTCACCCACGACTGGATGGTGTTCGTACGCGGTCCGGAGCACAGTAACATACAGCACTT TGTGGAGAAAGTCGTCTTCCACTTGCACGAAAGCTTTCCTAGGCCAAAAAGAGTGTGCAAAGATCCACCT TACAAAGTAGAAGAATCTGGGTATGCTGGTTTCATTTTGCCAATTGAAGTTTATTTTAAAAACAAGGAAG AACCTAGGAAAGTCCGCTTTGATTATGACTTATTCCTGCATCTTGAAGGCCATCCACCAGTGAATCACCT CCGCTGTGAAAAGCTAACTTTCAACAACCCCACAGAGGACTTTAGGAGAAAGTTGCTGAAGGCAGGAGGG GACCCTAATAGGAGTATTCATACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGCA GCAGCAGCAGTAGCAGCAGCAGCAGCGGCAGCAGCAGCAGCAGTAGCAGCAGCAGTAGCAGCAGCAGCAG CAGCAGTAGTACCAGTTTTTCAAAGCCTCACAAATTAATGAAGGAGCACAAGGAAAAACCTTCTAAAGAC TCCAGAGAACATAAAAGTGCCTTCAAAGAACCTTCCAGGGATCACAACAAATCTTCCAAAGAATCCTCTA AGAAACCCAAAGAAAATAAACCACTGAAAGAAGAGAAAATAGTTCCTAAGATGGCCTTCAAGGAACCTAA ACCCATGTCAAAAGAGCCAAAACCAGATAGTAACTTACTCACCATCACCAGTGGACAAGATAAGAAGGCT CCTAGTAAAAGGCCGCCCATTTCAGATTCTGAAGAACTCTCAGCCAAAAAAAGGAAAAAGAGTAGCTCAG AGGCTTTATTTAAAAGTTTTTCTAGCGCACCACCACTGATACTCACTTGTTCTGCTGACAAAAAACAGAT AAAAGATAAATCTCATGTCAAGATGGGAAAGGTCAAAATTGAAAGTGAGACATCAGAGAAGAAGAAATCA ACGTTACCGCCATTTGATGATATTGTGGATCCCAATGATTCAGATGTGGAGGAGAATATATCCTCTAAAT CTGATTCTGAACAACCCAGTCCTGCCAGCTCCAGCTCCAGCTCCAGCTCCAGCTTCACACCATCCCAGAC CAGGCAACAAGGTCCTTTGAGGTCTATAATGAAAGATCTGCATTCTGATGACAATGAGGAGGAATCAGAT GAAGTGGAGGATAACGACAATGACTCTGAAATGGAGAGGCCTGTAAATAGAGGAGGCAGCCGAAGTCGCA GAGTTAGCTTAAGTGATGGCAGCGATAGTGAAAGCAGTTCTGCTTCTTCACCCCTACATCACGAACCTCC ACCACCCTTACTAAAAACCAACAACAACCAGATTCTTGAAGTGAAAAGTCCAATAAAGCAAAGCAAATCA GATAAGCAAATAAAGAATGGTGAATGTGACAAGGCATACCTAGATGAACTGGTAGAGCTTCACAGAAGGT TAATGACATTGAGAGAAAGACACATTCTGCAGCAGATCGTGAACCTTATAGAAGAAACTGGACACTTTCA TATCACAAACACAACATTTGATTTTGATCTTTGCTCGCTGGACAAAACCACAGTCCGTAAACTACAGAGT TACCTGGAAACATCTGGAACATCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206283 representing NM_004529
Red=Cloning site Green=Tags(s) MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPP YKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGG DPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSGSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKD SREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKKA PSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKKKS TLPPFDDIVDPNDSDVEENISSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEESD EVEDNDNDSEMERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSKS DKQIKNGECDKAYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTVRKLQS YLETSGTS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004529 |
| ORF Size | 1704 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004529.1, NP_004520.1 |
| RefSeq Size | 3376 bp |
| RefSeq ORF | 1707 bp |
| Locus ID | 4300 |
| UniProt ID | P42568 |
| Domains | YEATS |
| Protein Families | Transcription Factors |
| Gene Summary | Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA (PubMed:20159561, PubMed:20471948, PubMed:25417107, PubMed:27105114, PubMed:27545619). Specifically recognizes and binds acylated histone H3, with a marked preference for histone H3 that is crotonylated (PubMed:25417107, PubMed:27105114, PubMed:27545619). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:25417107, PubMed:27105114, PubMed:27545619). Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr) (PubMed:27105114). Also recognizes and binds histone H3 acetylated at 'Lys-9' (H3K9ac), but with lower affinity than crotonylated histone H3 (PubMed:25417107, PubMed:27105114). In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones (PubMed:27105114, PubMed:27545619).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206283 | MLLT3 (Myc-DDK-tagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (MLLT3) |
CNY 4,232.00 |
|
| RC206283L1 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), Myc-DDK-tagged |
CNY 6,632.00 |
|
| RC206283L2 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), mGFP tagged |
CNY 5,890.00 |
|
| RC206283L3 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206283L4 | Lenti ORF clone of Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3 (MLLT3), mGFP tagged |
CNY 6,632.00 |
|
| SC127943 | MLLT3 (untagged)-Human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila), translocated to, 3 (MLLT3) |
CNY 4,240.00 |
