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RNF190 (MARCH10) (NM_152598) Human Tagged ORF Clone
CAT＃: RG206212
- TrueORF®
41343 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1
ORF Plasmid: DDK
"NM_152598" in other vectors (6)
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CNY 6,496.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MARCH-X; MARCH10; RNF190 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206212 representing NM_152598.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGCATGACGCAAGGGACAGGCAGAAGTTCTTCAGCGATGTTCAGTATCTGCGGGACATGCAGCAT AAGGTGGACTCTGAGTATCAGGCTTGTCTGAGACGACAGGAATATAGAAGAGACCCAAATGAGAAGAAA CGCGATCAGTTTTGGGGGCAAGAGACAAGTTTTGAGAGATCCCGGTTTTCTAGCAGGTCATCTTCCAAA CAGAGTTCTAGTGAGGAGGATGCTCTAACTGAACCAAGGTCATCTATCAAGATATCTGCATTTAAGTGT GACTCCAAACTTCCAGCAATTGACCAAACATCAGTCAAGCAGAAACATAAAAGTACCATGACTGTAAGG AAAGCAGAGAAAGTGGACCCCAGCGAACCCTCTCCAGCAGACCAAGCACCAATGGTTTTATTAAGAAAG AGGAAGCCAAACCTGAGGAGATTTACAGTCAGCCCAGAATCGCACAGCCCGAGAGCATCTGGGGACAGA AGCAGACAGAAACAGCAGTGGCCTGCAAAGGTGCCGGTTCCCAGGGGAGCAGATCAAGTAGTTCAACAA GAAGGCCTGATGTGTAACACTAAGCTGAAGAGGCCAAATCAAGAGAGAAGAAACTTGGTCCCATCGTCA CAGCCAATGACTGAGAACGCCCCTGATAGAGCCAAAAAAGGAGACCCGAGTGCTCCTTCCCAGAGTGAG CTGCATCCAGCCCTGTCCCAGGCCTTCCAAGGAAAAAATAGTCCTCAAGTATTGAGTGAGTTCTCGGGG CCACCACTCACACCCACCACTGTAGGAGGGCCAAGAAAGGCATCATTTAGGTTCCGAGATGAAGACTTT TATTCCATTTTGTCATTGAACAGCAGAAGAGAAAGCGATGACACTGAAGAGGAAACCCAGTCTGAAGAA TGTCTGTGGGTTGGTGTGCGCTCACCCTGTTCTCCTTCCCATCACAAAAGAAGTAGATTTGGGGGGACA TCGACCCCTCAGGCCAAAAATAAAAATTTTGAAGAAAATGCTGAAAATTGCAGAGGTCATTCTTCAAGA AGAAGTGAGCCCAGTCATGGCTCATTGAGAATAAGCAATGCAATGGAGCCAGCAACAGAGCGGCCTTCT GCTGGGCAAAGGTTGTCCCAAGACCCCGGGCTGCCTGATAGGGAATCTGCTACAGAGAAGGACAGAGGT GGCAGTGAAAATGCGAAAAAGAGCCCTCTTTCGTGGGACACCAAATCCGAGCCCAGACAAGAGGTTGGT GTCAATGCTGAAAATGTATGGAGTGATTGTATTTCTGTGGAACATAGGCCTGGCACCCATGATTCTGAA GGATACTGGAAAGACTATTTAAACAGTTCTCAAAATTCTCTTGACTACTTTATTTCTGGCAGACCAATA TCTCCAAGATCATCAGTGAATTCATCCTATAACCCTCCTGCATCATTCATGCATTCTGCTCTGAGAGAT GATATTCCAGTAGACTTGTCAATGTCATCGACTTCAGTTCACAGCTCAGACTCAGAGGGAAACTCAGGG TTTCATGTTTGCCAACCACTGTCTCCCATTAGAAATAGGACTCCATTTGCCAGTGCCGAAAACCATAAT TATTTCCCAGTAAACAGTGCACACGAATTTGCTGTCAGGGAAGCAGAAGATACTACTTTAACAAGCCAG CCTCAGGGGGCTCCACTATATACAGATCTCTTACTAAATCCACAGGGCAATTTGTCCTTAGTGGATTCT TCCAGCTCCTCTCCATCAAGAATGAACTCAGAAGGCCATTTGCATGTGTCTGGGTCTCTGCAAGAAAAT ACACCATTTACTTTCTTTGCAGTGTCTCATTTCCCAAATCAAAATGATAATGGGAGCAGGATGGCAGCC TCTGGTTTCACAGATGAAAAGGAAACCAGTAAGATAAAGGCAGACCCTGAGAAACTCAAGAAATTGCAA GAAAGTCTCCTGGAGGAGGACTCCGAGGAGGAGGGAGACTTGTGTCGCATCTGTCAGATAGCCGGGGGT TCCCCAAGCAACCCCCTCCTGGAGCCTTGCGGCTGTGTGGGAAGCCTGCAGTTTGTTCATCAAGAGTGC CTGAAAAAGTGGCTGAAAGTGAAAATAACATCAGGAGCAGATCTTGGTGCCGTGAAGACCTGTGAGATG TGTAAGCAAGGCCTGCTGGTTGACCTGGGTGACTTTAACATGATTGAGTTCTACCAGAAGCACCAGCAA TCTCAGGCACAAAACGAGCTGATGAATTCAGGCTTGTACCTGGTGCTGCTGCTTCACCTCTATGAGCAG AGGTTTGCAGAACTCATGAGGCTCAACCACAACCAGGTGGAAAGAGAGAGGTTGTCAAGAAATTACCCA CAACCCAGAACAGAGGAAAATGAAAATTCGGAGTTGGGAGATGGAAATGAAGGCAGCATTTCTCAAAGC CAGGTCGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC >Peptide sequence encoded by RG206212
Blue=ORF Red=Cloning site Green=Tag(s) MLHDARDRQKFFSDVQYLRDMQHKVDSEYQACLRRQEYRRDPNEKKRDQFWGQETSFERSRFSSRSSSK QSSSEEDALTEPRSSIKISAFKCDSKLPAIDQTSVKQKHKSTMTVRKAEKVDPSEPSPADQAPMVLLRK RKPNLRRFTVSPESHSPRASGDRSRQKQQWPAKVPVPRGADQVVQQEGLMCNTKLKRPNQERRNLVPSS QPMTENAPDRAKKGDPSAPSQSELHPALSQAFQGKNSPQVLSEFSGPPLTPTTVGGPRKASFRFRDEDF YSILSLNSRRESDDTEEETQSEECLWVGVRSPCSPSHHKRSRFGGTSTPQAKNKNFEENAENCRGHSSR RSEPSHGSLRISNAMEPATERPSAGQRLSQDPGLPDRESATEKDRGGSENAKKSPLSWDTKSEPRQEVG VNAENVWSDCISVEHRPGTHDSEGYWKDYLNSSQNSLDYFISGRPISPRSSVNSSYNPPASFMHSALRD DIPVDLSMSSTSVHSSDSEGNSGFHVCQPLSPIRNRTPFASAENHNYFPVNSAHEFAVREAEDTTLTSQ PQGAPLYTDLLLNPQGNLSLVDSSSSSPSRMNSEGHLHVSGSLQENTPFTFFAVSHFPNQNDNGSRMAA SGFTDEKETSKIKADPEKLKKLQESLLEEDSEEEGDLCRICQIAGGSPSNPLLEPCGCVGSLQFVHQEC LKKWLKVKITSGADLGAVKTCEMCKQGLLVDLGDFNMIEFYQKHQQSQAQNELMNSGLYLVLLLHLYEQ RFAELMRLNHNQVERERLSRNYPQPRTEENENSELGDGNEGSISQSQVV TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_152598 |
| ORF Size | 2424 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_152598.4 |
| RefSeq Size | 3090 bp |
| RefSeq ORF | 2427 bp |
| Locus ID | 162333 |
| UniProt ID | Q8NA82 |
| MW | 90.5 kDa |
| Gene Summary | MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206212 | MARCH10 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1 |
CNY 4,896.00 |
|
| RC206212L1 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1, Myc-DDK-tagged |
CNY 7,296.00 |
|
| RC206212L2 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206212L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206212L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC306455 | 41343 (untagged)-Human membrane-associated ring finger (C3HC4) 10 (MARCH10), transcript variant 1 |
CNY 7,220.00 |
