United States
Germany
Japan
United Kingdom
China
WDR85 (DPH7) (NM_138778) Human Tagged ORF Clone
CAT＃: RG205735
- TrueORF®
WDR85 (tGFP-tagged) - Human WD repeat domain 85 (WDR85)
ORF Plasmid: DDK
"NM_138778" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,256.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C9orf112; RRT2; WDR85 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205735 representing NM_138778
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGGCTGTTTCGCCCTGCAAACGGTGGACACCGAGCTGACCGCGGACTCGGTGGAGTGGTGCCCGC TGCAAGGCTGCAGGCACCTGCTGGCGTGCGGGACCTACCAGCTGCGGCGGCCGGAGGACCGGCCTGCCGG CCCCCAGAACAAGGGTGGAATGGAAGTTAAGGAGCCTCAGGTCCGTTTAGGCCGTCTCTTCCTGTACAGT TTCAATGACAACAACTCTATTCACCCTCTGGTCGAGGTCCAAAGAAAAGATACTTCTGCAATCCTGGACA TGAAATGGTGTCACATCCCGGTGGCTGGACATGCCCTCTTGGGCTTGGCAGATGCCAGTGGATCCATACA ACTGCTCCGCCTGGTGGAATCTGAGAAGAGCCACGTGCTGGAGCCATTGTCCAGCCTTGCCCTGGAGGAG CAGTGTCTGGCTTTGTCCCTAGATTGGTCCACTGGGAAAACTGGAAGGGCCGGGGACCAGCCCTTGAAGA TCATCAGCAGTGACTCCACAGGGCAGCTCCACCTCCTGATGGTGAATGAGACGAGGCCCAGGCTGCAGAA AGTGGCCTCATGGCAGGCACATCAATTCGAGGCCTGGATTGCTGCTTTCAATTACTGGCATCCAGAAATT GTGTATTCAGGGGGCGACGATGGCCTTCTGAGGGGCTGGGACACCAGGGTACCCGGCAAATTTCTCTTCA CCAGCAAAAGACACACCATGGGTGTGTGCAGCATCCAGAGCAGCCCTCATCGGGAGCACATCCTGGCCAC GGGAAGCTATGATGAACACATCCTACTGTGGGACACACGAAACATGAAGCAGCCGTTGGCAGATACGCCT GTGCAGGGTGGGGTATGGAGAATCAAGTGGCACCCTTTCCACCACCACCTGCTCCTGGCCGCCTGCATGC ACAGTGGCTTTAAGATCCTCAACTGCCAAAAGGCAATGGAGGAGAGGCAGGAGGCGACGGTCCTGACATC TCACACATTGCCCGACTCGCTGGTGTATGGAGCCGACTGGTCCTGGCTGCTCTTCCGTTCTCTGCAGCGG GCCCCCTCGTGGTCCTTTCCTAGCAACCTAGGAACCAAGACGGCAGACCTGAAGGGTGCAAGCGAGTTGC CAACACCCTGTCATGAATGCAGAGAGGATAACGATGGGGAGGGCCATGCCAGACCCCAGAGTGGAATGAA GCCACTCACAGAGGGCATGAGGAAGAATGGCACCTGGCTGCAGGCTACAGCAGCCACCACACGTGACTGT GGCGTGAACCCAGAAGAAGCAGACTCAGCCTTCAGCCTCCTGGCCACCTGCTCCTTCTATGACCATGCGC TCCACCTCTGGGAGTGGGAGGGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205735 representing NM_138778
Red=Cloning site Green=Tags(s) MMGCFALQTVDTELTADSVEWCPLQGCRHLLACGTYQLRRPEDRPAGPQNKGGMEVKEPQVRLGRLFLYS FNDNNSIHPLVEVQRKDTSAILDMKWCHIPVAGHALLGLADASGSIQLLRLVESEKSHVLEPLSSLALEE QCLALSLDWSTGKTGRAGDQPLKIISSDSTGQLHLLMVNETRPRLQKVASWQAHQFEAWIAAFNYWHPEI VYSGGDDGLLRGWDTRVPGKFLFTSKRHTMGVCSIQSSPHREHILATGSYDEHILLWDTRNMKQPLADTP VQGGVWRIKWHPFHHHLLLAACMHSGFKILNCQKAMEERQEATVLTSHTLPDSLVYGADWSWLLFRSLQR APSWSFPSNLGTKTADLKGASELPTPCHECREDNDGEGHARPQSGMKPLTEGMRKNGTWLQATAATTRDC GVNPEEADSAFSLLATCSFYDHALHLWEWEGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_138778 |
| ORF Size | 1356 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_138778.5 |
| RefSeq Size | 1848 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 92715 |
| UniProt ID | Q9BTV6 |
| Domains | WD40 |
| Gene Summary | Diphthamide is a post-translationally modified histidine residue present in elongation factor 2, and is the target of diphtheria toxin. This gene encodes a protein that contains a WD-40 domain, and is thought to be involved in diphthamide biosynthesis. A similar protein in yeast functions as a methylesterase, converting methylated diphthine to diphthine, which can then undergo amidation to produce diphthamide. [provided by RefSeq, Oct 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205735 | WDR85 (Myc-DDK-tagged)-Human WD repeat domain 85 (WDR85) |
CNY 3,656.00 |
|
| RC205735L3 | Lenti ORF clone of Human WD repeat domain 85 (WDR85), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205735L4 | Lenti ORF clone of Human WD repeat domain 85 (WDR85), mGFP tagged |
CNY 5,890.00 |
|
| SC319616 | WDR85 (untagged)-Human WD repeat domain 85 (WDR85) |
CNY 3,656.00 |
