ENO3 (NM_053013) Human Tagged ORF Clone
CAT#: RG205684
- TrueORF®
ENO3 (tGFP-tagged) - Human enolase 3 (beta, muscle) (ENO3), transcript variant 2
ORF Plasmid: DDK
"NM_053013" in other vectors (6)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GSD13; MSE |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205684 representing NM_053013
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAGGGGCAACCCCACGGTGGAGGTGGACC TGCACACGGCCAAGGGCCGATTCCGAGCAGCTGTGCCCAGTGGGGCTTCCACGGGTATCTATGAGGCTCT GGAACTAAGAGACGGAGACAAAGGCCGCTACCTGGGGAAAGGAGTCCTGAAGGCTGTGGAGAACATCAAC AGTACTCTGGGCCCTGCTCTGCTGCAAAAGAAACTAAGCGTTGCGGATCAAGAAAAAGTTGACAAATTTA TGATTGAGCTAGATGGGACCGAGAATAAGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTCCTTGGC CGTGTGTAAGGCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCAGATCTCGCTGGGAAC CCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGGGGGCTCCCATGCTGGAAACAAGCTGG CCATGCAGGAGTTCATGATTCTGCCTGTGGGAGCCAGCTCCTTCAAGGAAGCCATGCGCATTGGCGCCGA GGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTATGGGAAGGATGCCACCAATGTGGGTGATGAA GGTGGCTTCGCACCCAACATCCTGGAGAACAATGAGGCCCTGGAGCTGCTGAAGACGGCCATCCAGGCGG CTGGTTACCCAGACAAGGTGGTGATCGGCATGGATGTGGCAGCATCTGAGTTCTATCGCAATGGGAAGTA CGATCTTGACTTCAAGTCGCCTGATGATCCCGCACGGCACATCACTGGGGAGAAGCTCGGAGAGCTGTAT AAGAGCTTTATCAAGAACTATCCTGTGGTCTCCATCGAAGACCCCTTTGACCAGGATGACTGGGCCACTT GGACCTCCTTCCTCTCGGGGGTGAACATCCAGATTGTGGGGGATGACTTGACAGTCACCAACCCCAAGAG GATTGCCCAGGCCGTTGAGAAGAAGGCCTGCAACTGTCTGCTGCTGAAGGTCAACCAGATCGGCTCGGTG ACCGAATCGATCCAGGCGTGCAAACTGGCTCAGTCTAATGGCTGGGGGGTGATGGTGAGCCACCGCTCTG GGGAGACTGAGGACACATTCATTGCTGACCTTGTGGTGGGGCTCTGCACAGGACAGATCAAGACTGGCGC CCCCTGCCGCTCGGAGCGTCTGGCCAAATACAACCAACTCATGAGGATCGAGGAGGCTCTTGGGGACAAG GCAATCTTTGCTGGACGCAAGTTCCGTAACCCGAAGGCCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205684 representing NM_053013
Red=Cloning site Green=Tags(s) MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENIN STLGPALLQKKLSVADQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGN PDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDE GGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELY KSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSV TESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDK AIFAGRKFRNPKAK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_053013 |
ORF Size | 1302 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_053013.2 |
RefSeq Size | 1452 bp |
RefSeq ORF | 1305 bp |
Locus ID | 2027 |
UniProt ID | P13929 |
Domains | enolase |
Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation |
Gene Summary | This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205684 | ENO3 (Myc-DDK-tagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 2 |
CNY 3,656.00 |
|
RC205684L1 | Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC205684L2 | Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC205684L3 | Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205684L4 | Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC319614 | ENO3 (untagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 2 |
CNY 3,656.00 |