LRRC50 (DNAAF1) (NM_178452) Human Tagged ORF Clone
CAT#: RG205452
- TrueORF®
DNAAF1 (tGFP-tagged) - Human leucine rich repeat containing 50 (LRRC50)
ORF Plasmid: DDK
"NM_178452" in other vectors (4)
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CNY 6,912.00
Cited in 1 publication. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CILD13; DAU1; LRRC50; ODA7; swt |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG205452 representing NM_178452
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCCTGAGCCCTCGGAGCCTGCGACAGGTGGTGCAGCAGAGCTGGATTGCGCGCAGGAGCCCGGCG TGGAGGAGTCTGCGGGTGACCACGGGAGCGCAGGCCGAGGGGGCTGCAAGGAAGAAATTAATGATCCTAA GGAAATATGTGTGGGTTCTTCTGACACATCCTACCACAGCCAGCAGAAACAGAGTGGTGATAATGGGTCA GGTGGTCACTTCGCACACCCAAGAGAAGACAGGGAAGATCGGGGCCCCAGAATGACTAAAAGTTCCCTGC AAAAACTCTGCAAGCAGCACAAGCTTTATATTACCCCAGCATTGAATGATACGCTGTATTTACACTTTAA AGGTTTTGATCGCATTGAGAACCTGGAAGAGTACACAGGGCTGCGCTGTCTCTGGCTGCAGAGCAATGGA ATACAGAAAATCGAAAACCTGGAGGCCCAAACTGAGTTGCGTTGCCTCTTCTTGCAAATGAACTTGCTCC GTAAAATTGAGAACCTGGAACCTCTGCAGAAACTGGATGCTCTTAACCTCAGCAACAATTACATCAAGAC CATTGAAAACCTCTCCTGCCTCCCAGTCCTGAACACATTGCAGATGGCCCACAATCACCTGGAGACCGTG GAGGACATTCAGCATCTACAAGAGTGTTTGAGGCTTTGTGTCCTTGACCTTTCGCACAACAAGCTGAGTG ACCCGGAGATCCTGAGCATTCTGGAAAGCATGCCCGATTTGCGTGTACTGAATTTGATGGGAAACCCGGT TATCAGACAGATTCCTAATTACAGAAGGACAGTCACTGTACGACTAAAGCACTTAACATACCTGGATGAT AGACCAGTGTTTCCAAAGGACAGAGCTTGTGCGGAGGCCTGGGCTAGGGGAGGGTACGCAGCTGAAAAGG AGGAGAGACAGCAGTGGGAGAGCAGGGAGCGGAAGAAGATCACAGACAGCATTGAAGCCTTGGCCATGAT CAAGCAGCGGGCAGAGGAGAGGAAAAGACAGAGAGAGAGTCAAGAGAGAGGGGAGATGACATCTTCAGAT GATGGTGAGAATGTGCCCGCCAGTGCGGAAGGCAAGGAGGAGCCTCCCGGGGACAGAGAAACAAGGCAGA AGATGGAGCTATTTGTTAAGGAAAGCTTTGAGGCCAAGGACGAGCTCTGCCCGGAAAAGCCAAGTGGAGA GGAGCCGCCTGTGGAGGCTAAAAGAGAGGATGGAGGTCCAGAGCCAGAGGGGACCCTCCCAGCTGAGACC CTGCTACTGTCGTCACCTGTGGAGGTTAAAGGAGAGGACGGAGATGGAGAGCCAGAGGGGACCCTCCCAG CTGAGGCCCCACCACCCCCGCCACCTGTGGAGGTTAAAGGAGAGGATGGAGATCAAGAGCCAGAGGGGAC CCTCCCAGCTGAGACCCTGCTACTGTCACCGCCTGTGAAGGTTAAAGGAGAGGATGGAGATCGAGAGCCA GAGGGGACCCTCCCAGCTGAGGCCCCACCACCACCGCCCCTGGGAGCTGCCAGGGAAGAACCGACTCCCC AGGCTGTGGCCACTGAGGGTGTATTCGTTACAGAACTTGATGGAACGAGAACGGAAGATTTAGAAACCAT TAGACTGGAGACAAAGGAGACATTCTGCATTGATGACCTACCTGACTTGGAAGATGATGATGAAACAGGC AAATCTCTGGAAGACCAGAATATGTGCTTTCCGAAGATTGAGGTCATCTCGAGCTTGAGTGATGACAGTG ACCCTGAACTGGACTACACGTCACTCCCTGTGCTGGAAAACCTCCCCACAGACACTCTGTCAAATATATT TGCAGTCTCTAAAGACACCTCAAAGGCGGCTCGGGTGCCCTTCACAGACATCTTTAAAAAAGAAGCTAAG AGGGACTTGGAAATCCGAAAACAAGACACCAAGTCCCCAAGACCCCTGATCCAGGAGCTCAGCGACGAGG ACCCCTCTGGCCAGCTACTGATGCCCCCCACCTGCCAAAGAGATGCTGCACCACTCACTTCCAGTGGAGA CAGGGACAGCGACTTCCTTGCAGCCTCTTCTCCGGTGCCGACTGAGAGCGCCGCCACACCCCCAGAGACG TGTGTCGGAGTTGCCCAGCCCAGCCAAGCTCTGCCCACGTGGGACCTCACTGCATTCCCAGCACCGAAAG CATCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205452 representing NM_178452
Red=Cloning site Green=Tags(s) MHPEPSEPATGGAAELDCAQEPGVEESAGDHGSAGRGGCKEEINDPKEICVGSSDTSYHSQQKQSGDNGS GGHFAHPREDREDRGPRMTKSSLQKLCKQHKLYITPALNDTLYLHFKGFDRIENLEEYTGLRCLWLQSNG IQKIENLEAQTELRCLFLQMNLLRKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNHLETV EDIQHLQECLRLCVLDLSHNKLSDPEILSILESMPDLRVLNLMGNPVIRQIPNYRRTVTVRLKHLTYLDD RPVFPKDRACAEAWARGGYAAEKEERQQWESRERKKITDSIEALAMIKQRAEERKRQRESQERGEMTSSD DGENVPASAEGKEEPPGDRETRQKMELFVKESFEAKDELCPEKPSGEEPPVEAKREDGGPEPEGTLPAET LLLSSPVEVKGEDGDGEPEGTLPAEAPPPPPPVEVKGEDGDQEPEGTLPAETLLLSPPVKVKGEDGDREP EGTLPAEAPPPPPLGAAREEPTPQAVATEGVFVTELDGTRTEDLETIRLETKETFCIDDLPDLEDDDETG KSLEDQNMCFPKIEVISSLSDDSDPELDYTSLPVLENLPTDTLSNIFAVSKDTSKAARVPFTDIFKKEAK RDLEIRKQDTKSPRPLIQELSDEDPSGQLLMPPTCQRDAAPLTSSGDRDSDFLAASSPVPTESAATPPET CVGVAQPSQALPTWDLTAFPAPKAS TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_178452 |
ORF Size | 2175 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178452.6 |
RefSeq Size | 2451 bp |
RefSeq ORF | 2178 bp |
Locus ID | 123872 |
UniProt ID | Q8NEP3 |
Gene Summary | The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans
,Miao, C;Jiang, Q;Li, H;Zhang, Q;Bai, B;Bao, Y;Zhang, T;,
G3 (Bethesda) 2016
,PubMed ID 27543293
[DNAAF1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205452 | DNAAF1 (Myc-DDK-tagged)-Human leucine rich repeat containing 50 (LRRC50) |
CNY 5,312.00 |
|
RC205452L3 | Lenti ORF clone of Human leucine rich repeat containing 50 (LRRC50), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205452L4 | Lenti ORF clone of Human leucine rich repeat containing 50 (LRRC50), mGFP tagged |
CNY 5,890.00 |
|
SC307182 | DNAAF1 (untagged)-Human leucine rich repeat containing 50 (LRRC50) |
CNY 7,220.00 |