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PJA2 (NM_014819) Human Tagged ORF Clone
CAT＃: RG205204
- TrueORF®
PJA2 (tGFP-tagged) - Human praja ring finger 2 (PJA2)
ORF Plasmid: DDK
"NM_014819" in other vectors (6)
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CNY 6,784.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Neurodap1; RNF131 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG205204 representing NM_014819
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACAGTACACTGAAAAGGAGCCAGCAGCAATGGACCAAGAATCTGGTAAGGCTGTCTGGCCCAAAC CAGCAGGAGGGTATCAGACAATTACAGGCAGGAGATATGGAAGAAGACATGCTTATGTCAGTTTTAAACC ATGTATGACCAGACATGAAAGAAGCTTAGGTCGGGCTGGTGATGACTATGAAGTGTTGGAACTAGATGAT GTTCCAAAGGAAAATTCCTCAGGTTCCAGTCCTTTGGATCAAGTTGATTCTTCTTTACCAAGTGAACCTA TATTTGAAAAAAGTGAAACAGAAATTCCCACTTGTGGTTCAGCATTGAATCAAACCACTGAGAGCAGTCA ATCCTTTGTTGCAGTACATCACAGTGAGGAAGGCAGGGATACCTTAGGAAGCAGTACAAATCTTCATAAT CACTCTGAGGGAGAGTATATTCCAGGAGCTTGTAGTGCTTCAAGTGTCCAAAATGGAATTGCATTGGTTC ATACAGACTCTTATGATCCAGATGGCAAACATGGAGAAGATAATGACCATCTTCAACTTTCTGCAGAAGT CGTGGAAGGTAGTAGATACCAGGAATCATTAGGCAATACAGTATTTGAGTTGGAAAACAGAGAGGCAGAG GCATACACTGGTCTTTCACCACCAGTTCCCTCATTTAACTGTGAAGTAAGAGATGAGTTTGAAGAGTTAG ATTCTGTACCATTAGTGAAAAGTTCTGCTGGTGATACTGAGTTTGTCCATCAGAATAGCCAGGAAATTCA GAGGTCTTCTCAAGATGAAATGGTTAGTACGAAACAACAAAATAATACTAGCCAGGAAAGACAGACAGAA CATTCACCTGAAGATGCAGCCTGTGGTCCAGGGCATATTTGTAGTGAACGAAATACCAATGATAGGGAAA AGAACCATGGAAGTTCTCCTGAACAGGTAGTGAGGCCAAAAGTTAGAAAACTGATAAGTTCAAGCCAGGT GGACCAAGAAACAGGTTTTAATAGGCATGAGGCGAAACAAAGAAGTGTTCAAAGATGGAGAGAGGCTTTG GAAGTTGAGGAAAGTGGCTCAGATGACCTCTTAATAAAATGTGAAGAATATGATGGAGAGCATGACTGTA TGTTCTTGGATCCACCATACTCAAGAGTTATTACACAAAGGGAAACAGAAAATAACCAAATGACATCAGA AAGTGGAGCCACAGCGGGAAGGCAAGAAGTGGATAACACCTTTTGGAATGGCTGTGGAGATTATTACCAA CTCTATGACAAAGATGAAGATAGTTCTGAATGCAGTGATGGGGAATGGTCTGCTTCTTTGCCTCATCGAT TTTCTGGTACAGAAAAAGATCAATCCTCAAGTGATGAAAGCTGGGAGACTCTGCCAGGAAAAGATGAGAA TGAACCTGAGCTACAAAGTGATAGCAGTGGCCCTGAAGAAGAAAACCAAGAATTATCTCTTCAGGAAGGG GAACAGACATCCTTGGAAGAGGGAGAAATTCCTTGGTTACAGTACAATGAAGTCAATGAAAGCAGCAGTG ATGAGGGAAATGAACCTGCCAATGAATTTGCACAGCCAGCTTTCATGTTGGATGGTAACAATAACCTGGA GGATGACTCCAGTGTGAGTGAAGACTTAGATGTGGATTGGAGCATATTTGATGGCTTTGCAGATGGACTA GGAGTTGCTGAAGCTATTTCATATGTGGATCCTCAGTTCCTTACATACATGGCACTAGAAGAACGCTTAG CCCAGGCTATGGAGACTGCTCTGGCCCATTTAGAGTCTCTTGCAGTGGATGTTGAGGTGGCCAATCCACC AGCTAGTAAGGAAAGCATTGATGGTCTTCCAGAGACCCTTGTTCTTGAAGATCACACTGCTATTGGTCAG GAACAATGCTGTCCAATCTGTTGCAGTGAGTATATTAAGGATGATATAGCAACAGAGTTGCCCTGTCACC ATTTCTTTCACAAACCTTGTGTCTCAATTTGGCTACAAAAGTCGGGAACATGCCCTGTGTGCCGCCGTCA TTTCCCACCTGCGGTTATTGAAGCATCTGCAGCTCCTTCCTCTGAGCCTGATCCTGATGCCCCACCTTCG AATGACAGTATTGCAGAAGCACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG205204 representing NM_014819
Red=Cloning site Green=Tags(s) MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDD VPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHN HSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAE AYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTE HSPEDAACGPGHICSERNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREAL EVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQ LYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEG EQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSIFDGFADGL GVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQ EQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPS NDSIAEAP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014819 |
| ORF Size | 2124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014819.3 |
| RefSeq Size | 4789 bp |
| RefSeq ORF | 2127 bp |
| Locus ID | 9867 |
| UniProt ID | O43164 |
| Domains | RING |
| Protein Families | Druggable Genome |
| Gene Summary | Has E2-dependent E3 ubiquitin-protein ligase activity. Responsible for ubiquitination of cAMP-dependent protein kinase type I and type II-alpha/beta regulatory subunits and for targeting them for proteasomal degradation. Essential for PKA-mediated long-term memory processes. Through the ubiquitination of MFHAS1, positively regulates the TLR2 signaling pathway that leads to the activation of the downstream p38 and JNK MAP kinases and promotes the polarization of macrophages toward the pro-inflammatory M1 phenotype (PubMed:28471450).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205204 | PJA2 (Myc-DDK-tagged)-Human praja ring finger 2 (PJA2) |
CNY 5,184.00 |
|
| RC205204L1 | Lenti ORF clone of Human praja ring finger 2 (PJA2), Myc-DDK-tagged |
CNY 7,584.00 |
|
| RC205204L2 | Lenti ORF clone of Human praja ring finger 2 (PJA2), mGFP tagged |
CNY 7,700.00 |
|
| RC205204L3 | Lenti ORF clone of Human praja ring finger 2 (PJA2), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC205204L4 | Lenti ORF clone of Human praja ring finger 2 (PJA2), mGFP tagged |
CNY 7,700.00 |
|
| SC126706 | PJA2 (untagged)-Human praja ring finger 2 (PJA2) |
CNY 5,192.00 |
