Aquaporin 4 (AQP4) (NM_001650) Human Tagged ORF Clone
CAT#: RG204693
- TrueORF®
AQP4 (tGFP-tagged) - Human aquaporin 4 (AQP4), transcript variant a
ORF Plasmid: DDK
"NM_001650" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Cited in 3 publications. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | MIWC; WCH4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG204693 representing NM_001650
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACAGACCCACAGCAAGGCGGTGGGGTAAGTGTGGACCTTTGTGTACCAGAGAGAACATCATGG TGGCTTTCAAAGGGGTCTGGACTCAAGCTTTCTGGAAAGCAGTCACAGCGGAATTTCTGGCCATGCTTAT TTTTGTTCTCCTCAGCCTGGGATCCACCATCAACTGGGGTGGAACAGAAAAGCCTTTACCGGTCGACATG GTTCTCATCTCCCTTTGCTTTGGACTCAGCATTGCAACCATGGTGCAGTGCTTTGGCCATATCAGCGGTG GCCACATCAACCCTGCAGTGACTGTGGCCATGGTGTGCACCAGGAAGATCAGCATCGCCAAGTCTGTCTT CTACATCGCAGCCCAGTGCCTGGGGGCCATCATTGGAGCAGGAATCCTCTATCTGGTCACACCTCCCAGT GTGGTGGGAGGCCTGGGAGTCACCATGGTTCATGGAAATCTTACCGCTGGTCATGGTCTCCTGGTTGAGT TGATAATCACATTTCAATTGGTGTTTACTATCTTTGCCAGCTGTGATTCCAAACGGACTGATGTCACTGG CTCAATAGCTTTAGCAATTGGATTTTCTGTTGCAATTGGACATTTATTTGCAATCAATTATACTGGTGCC AGCATGAATCCCGCCCGATCCTTTGGACCTGCAGTTATCATGGGAAATTGGGAAAACCATTGGATATATT GGGTTGGGCCCATCATAGGAGCTGTCCTCGCTGGTGGCCTTTATGAGTATGTCTTCTGTCCAGATGTTGA ATTCAAACGTCGTTTTAAAGAAGCCTTCAGCAAAGCTGCCCAGCAAACAAAAGGAAGCTACATGGAGGTG GAGGACAACAGGAGTCAGGTAGAGACGGATGACCTGATTCTAAAACCTGGAGTGGTGCATGTGATTGACG TTGACCGGGGAGAGGAGAAGAAGGGGAAAGACCAATCTGGAGAGGTATTGTCTTCAGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204693 representing NM_001650
Red=Cloning site Green=Tags(s) MSDRPTARRWGKCGPLCTRENIMVAFKGVWTQAFWKAVTAEFLAMLIFVLLSLGSTINWGGTEKPLPVDM VLISLCFGLSIATMVQCFGHISGGHINPAVTVAMVCTRKISIAKSVFYIAAQCLGAIIGAGILYLVTPPS VVGGLGVTMVHGNLTAGHGLLVELIITFQLVFTIFASCDSKRTDVTGSIALAIGFSVAIGHLFAINYTGA SMNPARSFGPAVIMGNWENHWIYWVGPIIGAVLAGGLYEYVFCPDVEFKRRFKEAFSKAAQQTKGSYMEV EDNRSQVETDDLILKPGVVHVIDVDRGEEKKGKDQSGEVLSSV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001650 |
ORF Size | 969 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001650.7 |
RefSeq Size | 5216 bp |
RefSeq ORF | 972 bp |
Locus ID | 361 |
UniProt ID | P55087 |
Domains | MIP |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. Additional isoforms, resulting from the use of alternative in-frame translation initiation codons, have also been described. Recent studies provided evidence for translational readthrough in this gene, and expression of C-terminally extended isoforms via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Jun 2018] |
Citations (3)
The use of this cDNA Clones has been cited in the following citations: |
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Detection of aquaporin-4 antibody status of Thai patients with neuromyelitis optica spectrum disorders using a recombinant Escherichia coli aquaporin-4 enzyme-linked immunosorbent assay
,Kanjan, K;Siritho, S;Apiwattanakul, M;,
Clinical and Experimental Neuroimmunology
[AQP4]
|
Detection of NMO-IgG Antibodies in Thai NMO Patients Using a Recombinant E. coli AQP4-M23 ELISA
,Kanjun, K;Uawithya, P;,
J Physiol July 2014
[AQP4]
|
Anti-aquaporin-1 autoantibodies in patients with neuromyelitis optica spectrum disorders
,Tzartos, JS;Stergiou, C;Kilidireas, K;Zisimopoulou, P;Thomaidis, T;Tzartos, SJ;,
PLoS One. 2013 Sep 23;8(9):e74773
,PubMed ID 24086369
[AQP4]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204693 | AQP4 (Myc-DDK-tagged)-Human aquaporin 4 (AQP4), transcript variant a |
CNY 2,400.00 |
|
RC204693L1 | Lenti ORF clone of Human aquaporin 4 (AQP4), transcript variant a, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC204693L2 | Lenti ORF clone of Human aquaporin 4 (AQP4), transcript variant a, mGFP tagged |
CNY 4,800.00 |
|
RC204693L3 | Lenti ORF clone of Human aquaporin 4 (AQP4), transcript variant a, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC204693L4 | Lenti ORF clone of Human aquaporin 4 (AQP4), transcript variant a, mGFP tagged |
CNY 4,800.00 |
|
SC108945 | AQP4 (untagged)-Human aquaporin 4 (AQP4), transcript variant a |
CNY 2,400.00 |
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