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NUP133 (NM_018230) Human Tagged ORF Clone
CAT＃: RG204410
- TrueORF®
NUP133 (tGFP-tagged) - Human nucleoporin 133kDa (NUP133)
ORF Plasmid: DDK
"NM_018230" in other vectors (7)
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CNY 9,648.00
| Cited in 1 publication. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GAMOS8; hNUP133; NPHS18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG204410 representing NM_018230
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCAGCCGCCCCTTCTCCGCGGACCCCGGGTACCGGGTCCCGAAGGGGCCCGCTGGCCGGACTCG GGCCCGGCTCCACGCCCCGGACGGCTAGCAGGAAGGGTCTGCCCCTGGGGTCTGCAGTCAGCTCCCCAGT GCTCTTCTCGCCGGTCGGCCGGCGTAGCTCGCTAAGCTCGCGGGGAACACCAACACGAATGTTCCCACAC CACTCCATAACTGAGTCTGTGAACTATGATGTGAAAACGTTTGGATCTTCTCTTCCTGTTAAAGTCATGG AAGCCCTAACATTGGCTGAAGTCGATGACCAGCTGACCATTAACATAGATGAAGGTGGATGGGCTTGTCT GGTGTGCAAAGAGAAGCTCATTATTTGGAAGATTGCTCTGTCACCTATTACTAAGTTATCCGTTTGCAAA GAACTTCAGCTGCCACCTAGTGATTTCCACTGGAGTGCCGACTTAGTGGCTCTTTCTTACTCTTCTCCCT CAGGTGAAGCACATTCTACTCAGGCTGTTGCTGTCATGGTTGCCACCAGAGAAGGATCTATCCGCTATTG GCCAAGCCTTGCTGGTGAAGATACCTACACAGAGGCTTTTGTAGATTCGGGAGGTGATAAGACTTACAGT TTCCTAACAGCAGTGCAGGGAGGAAGTTTTATTTTGTCTTCATCAGGAAGCCAACTAATTCGGTTGATAC CTGAGAGCTCAGGAAAGATTCATCAGCATATCCTGCCTCAGGGGCAAGGCATGCTTTCAGGAATTGGTCG AAAAGTTTCTTCTCTTTTTGGAATTTTATCTCCTAGTAGTGATCTCACACTTTCAAGTGTTCTCTGGGAT AGAGAGAGATCAAGCTTTTATAGCCTGACGAGTTCAAACATCAGTAAATGGGAATTAGATGATTCTTCAG AAAAGCATGCATACAGTTGGGATATAAATAGAGCCCTGAAGGAAAACATTACCGATGCTATTTGGGGATC TGAAAGTAACTATGAAGCTATTAAAGAAGGAGTCAACATTCGATATTTGGACTTTAAGCAAAACTGTGAT GGGCTGGTGATTTTGGCAGCAGCATGGCACTCAGCAGACAATCCATGTCTCATCTATTACTCTCTGATAA CAATAGAAGATAATGGTTGCCAAATGTCAGATGCAGTTACTGTAGAAGTCACTCAATATAATCCACCTTT TCAGTCTGAAGACCTGATTTTGTGTCAGTTGACGGTCCCAAACTTTTCAAACCAGACTGCCTATCTGTAT AACGAAAGTGCTGTCTATGTGTGCTCCACAGGAACTGGGAAATTTTCTCTTCCCCAGGAGAAAATTGTCT TTAATGCACAAGGAGATAGTGTTTTAGGTGCTGGTGCCTGTGGTGGTGTTCCTATCATTTTTTCTAGAAA CAGTGGACTGGTGTCTATTACTTCAAGGGAAAATGTGTCTATATTGGCAGAAGACTTGGAAGGGTCTTTA GCATCTTCAGTTGCTGGACCAAACAGTGAGAGTATGATTTTTGAGACCACTACAAAGAATGAAACTATAG CCCAGGAAGATAAAATCAAGTTGCTGAAAGCTGCCTTTCTGCAATACTGCAGAAAAGATTTAGGTCATGC TCAAATGGTGGTTGATGAGCTCTTTTCCTCTCACTCTGATTTGGATTCTGATTCTGAACTAGACAGGGCA GTTACCCAAATCAGTGTAGACCTGATGGATGACTACCCAGCATCTGACCCACGGTGGGCTGAGTCTGTCC CTGAGGAAGCACCTGGGTTCAGCAATACGTCACTGATTATCCTTCACCAGCTAGAAGACAAGATGAAAGC TCACTCTTTTCTTATGGACTTTATTCATCAAGTTGGCTTATTTGGACGTCTAGGCAGTTTTCCAGTTAGA GGGACACCGATGGCCACTCGACTGTTGCTCTGTGAGCATGCCGAAAAGCTGTCAGCCGCCATTGTTCTCA AGAACCACCACTCCCGGCTTTCTGACCTTGTCAACACAGCCATATTGATTGCTTTGAACAAGAGGGAGTA TGAAATCCCATCCAACCTGACTCCTGCAGATGTCTTTTTCAGGGAGGTATCCCAAGTAGATACCATCTGT GAGTGCTTACTGGAGCATGAGGAGCAAGTCTTGAGGGATGCACCTATGGATTCCATTGAATGGGCTGAAG TGGTGATCAATGTGAACAATATTCTCAAGGATATGCTGCAGGCTGCTAGTCATTATCGCCAAAATAGAAA CTCTTTGTATAGAAGAGAAGAATCACTAGAAAAAGAACCTGAATATGTTCCATGGACGGCAACAAGTGGT CCTGGTGGCATCCGAACGGTAATAATACGCCAGCATGAGATTGTCCTGAAGGTGGCTTATCCACAGGCAG ACAGCAACCTCCGAAACATCGTGACCGAGCAGCTGGTAGCCCTGATCGATTGCTTCCTGGATGGTTATGT TTCTCAGCTTAAGTCTGTGGATAAATCCAGTAATCGGGAAAGATATGACAATCTGGAGATGGAATACCTA CAGAAAAGATCAGATCTCTTATCTCCTCTTCTTTCACTAGGCCAGTACCTGTGGGCTGCTTCTCTAGCAG AGAAATACTGTGACTTTGATATATTGGTACAAATGTGTGAGCAGACTGACAACCAGAGCCGACTCCAGCG CTACATGACCCAGTTTGCTGATCAGAATTTTTCAGACTTTCTCTTCCGTTGGTATCTGGAGAAAGGAAAG CGAGGCAAATTATTATCTCAGCCCATTTCTCAGCATGGACAGTTGGCAAATTTTTTGCAAGCTCATGAAC ATCTCAGCTGGTTACATGAAATTAATAGCCAAGAATTAGAAAAGGCTCATGCAACACTTCTGGGTTTGGC AAATATGGAAACTCGTTACTTTGCAAAGAAGAAAACCCTTCTTGGCTTGAGTAAATTGGCTGCATTAGCT TCAGACTTTTCAGAGGATATGCTACAAGAAAAAATTGAAGAAATGGCTGAGCAGGAGCGCTTTCTACTGC ATCAGGAGACCCTACCTGAACAGCTGCTGGCGGAGAAACAGCTAAATCTCAGTGCGATGCCAGTATTGAC TGCACCACAACTCATTGGTCTATATATCTGTGAAGAAAATAGAAGAGCTAATGAATATGATTTCAAGAAA GCTTTGGACTTGTTGGAATATATTGATGAGGAAGAAGATATAAATATAAATGATCTAAAACTGGAAATCC TTTGCAAAGCTCTTCAGAGAGATAACTGGTCCAGTTCTGATGGCAAAGATGATCCAATTGAAGTATCTAA AGACAGTATATTTGTGAAGATCTTACAGAAACTTTTAAAAGATGGCATTCAGCTCAGTGAGTACTTACCG GAGGTGAAAGACCTGCTACAAGCGGATCAGCTTGGAAGCTTAAAGTCCAATCCTTACTTCGAGTTTGTTT TGAAAGCAAATTATGAATATTATGTTCAGGGACAAATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG204410 representing NM_018230
Red=Cloning site Green=Tags(s) MFPAAPSPRTPGTGSRRGPLAGLGPGSTPRTASRKGLPLGSAVSSPVLFSPVGRRSSLSSRGTPTRMFPH HSITESVNYDVKTFGSSLPVKVMEALTLAEVDDQLTINIDEGGWACLVCKEKLIIWKIALSPITKLSVCK ELQLPPSDFHWSADLVALSYSSPSGEAHSTQAVAVMVATREGSIRYWPSLAGEDTYTEAFVDSGGDKTYS FLTAVQGGSFILSSSGSQLIRLIPESSGKIHQHILPQGQGMLSGIGRKVSSLFGILSPSSDLTLSSVLWD RERSSFYSLTSSNISKWELDDSSEKHAYSWDINRALKENITDAIWGSESNYEAIKEGVNIRYLDFKQNCD GLVILAAAWHSADNPCLIYYSLITIEDNGCQMSDAVTVEVTQYNPPFQSEDLILCQLTVPNFSNQTAYLY NESAVYVCSTGTGKFSLPQEKIVFNAQGDSVLGAGACGGVPIIFSRNSGLVSITSRENVSILAEDLEGSL ASSVAGPNSESMIFETTTKNETIAQEDKIKLLKAAFLQYCRKDLGHAQMVVDELFSSHSDLDSDSELDRA VTQISVDLMDDYPASDPRWAESVPEEAPGFSNTSLIILHQLEDKMKAHSFLMDFIHQVGLFGRLGSFPVR GTPMATRLLLCEHAEKLSAAIVLKNHHSRLSDLVNTAILIALNKREYEIPSNLTPADVFFREVSQVDTIC ECLLEHEEQVLRDAPMDSIEWAEVVINVNNILKDMLQAASHYRQNRNSLYRREESLEKEPEYVPWTATSG PGGIRTVIIRQHEIVLKVAYPQADSNLRNIVTEQLVALIDCFLDGYVSQLKSVDKSSNRERYDNLEMEYL QKRSDLLSPLLSLGQYLWAASLAEKYCDFDILVQMCEQTDNQSRLQRYMTQFADQNFSDFLFRWYLEKGK RGKLLSQPISQHGQLANFLQAHEHLSWLHEINSQELEKAHATLLGLANMETRYFAKKKTLLGLSKLAALA SDFSEDMLQEKIEEMAEQERFLLHQETLPEQLLAEKQLNLSAMPVLTAPQLIGLYICEENRRANEYDFKK ALDLLEYIDEEEDININDLKLEILCKALQRDNWSSSDGKDDPIEVSKDSIFVKILQKLLKDGIQLSEYLP EVKDLLQADQLGSLKSNPYFEFVLKANYEYYVQGQI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018230 |
| ORF Size | 3468 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018230.2, NP_060700.2 |
| RefSeq Size | 4170 bp |
| RefSeq ORF | 3471 bp |
| Locus ID | 55746 |
| UniProt ID | Q8WUM0 |
| Domains | Nup133 |
| Gene Summary | The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD
,Coyne, AN;Zaepfel, BL;Hayes, L;Fitchman, B;Salzberg, Y;Luo, EC;Bowen, K;Trost, H;Aigner, S;Rigo, F;Yeo, GW;Harel, A;Svendsen, CN;Sareen, D;Rothstein, JD;,
Neuron
,PubMed ID 32673563
[NUP133]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204410 | NUP133 (Myc-DDK-tagged)-Human nucleoporin 133kDa (NUP133) |
CNY 8,048.00 |
|
| RC204410L1 | Lenti ORF clone of Human nucleoporin 133kDa (NUP133), Myc-DDK-tagged |
CNY 11,780.00 |
|
| RC204410L2 | Lenti ORF clone of Human nucleoporin 133kDa (NUP133), mGFP tagged |
CNY 10,448.00 |
|
| RC204410L3 | Lenti ORF clone of Human nucleoporin 133kDa (NUP133), Myc-DDK-tagged |
CNY 11,780.00 |
|
| RC204410L4 | Lenti ORF clone of Human nucleoporin 133kDa (NUP133), mGFP tagged |
CNY 11,780.00 |
|
| SC113661 | NUP133 (untagged)-Human nucleoporin 133kDa (NUP133) |
CNY 10,450.00 |
|
| SC322454 | NUP133 (untagged)-Human nucleoporin 133kDa (NUP133) |
CNY 8,056.00 |
