NUP133 (NM_018230) Human Tagged Lenti ORF Clone
CAT#: RC204410L4
- LentiORF®
Lenti ORF clone of Human nucleoporin 133kDa (NUP133), mGFP tagged
"NM_018230" in other vectors (7)
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CNY 11,780.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | GAMOS8; hNUP133; NPHS18 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC204410).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_018230 |
ORF Size | 3468 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018230.2 |
RefSeq Size | 4170 bp |
RefSeq ORF | 3471 bp |
Locus ID | 55746 |
UniProt ID | Q8WUM0 |
Domains | Nup133 |
MW | 129 kDa |
Gene Summary | The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC204410 | NUP133 (Myc-DDK-tagged)-Human nucleoporin 133kDa (NUP133) |
CNY 8,048.00 |
|
RC204410L1 | Lenti ORF clone of Human nucleoporin 133kDa (NUP133), Myc-DDK-tagged |
CNY 11,780.00 |
|
RC204410L2 | Lenti ORF clone of Human nucleoporin 133kDa (NUP133), mGFP tagged |
CNY 10,448.00 |
|
RC204410L3 | Lenti ORF clone of Human nucleoporin 133kDa (NUP133), Myc-DDK-tagged |
CNY 11,780.00 |
|
RG204410 | NUP133 (tGFP-tagged) - Human nucleoporin 133kDa (NUP133) |
CNY 9,648.00 |
|
SC113661 | NUP133 (untagged)-Human nucleoporin 133kDa (NUP133) |
CNY 10,450.00 |
|
SC322454 | NUP133 (untagged)-Human nucleoporin 133kDa (NUP133) |
CNY 8,056.00 |