Gemin 4 (GEMIN4) (NM_015721) Human Tagged ORF Clone
CAT#: RG203733
- TrueORF®
GEMIN4 (tGFP-tagged) - Human gem (nuclear organelle) associated protein 4 (GEMIN4)
ORF Plasmid: DDK
"NM_015721" in other vectors (5)
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CNY 11,590.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HC56; HCAP1; HHRF-1; NEDMCR; p97 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG203733 representing NM_015721
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTAGGACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGC AGCTGTTCCACCCTAAGGCACTGGCAGAATTAACAAAGTCTGACTGGGAACGTGTTGGACGGCCCATCGT GGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTG ATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCGTCCGACACAGAGACACGGTGGCAGG AAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACCATCCTCTTCGAGCTGCTCAA ATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAA CTAGAGCGCTTTCTGGAACATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTCCTGGACGTCT GGTGGGAGGTGATGAAGCACAAGGGTCACCCGCAGGACCCCCTGCTCTCCCAGTTTAGTGCAATGGCCCA TAAGTACCTGCCTGCCTTAGATGAGTTCCCCCATCCTCCAAAGAGGCTTAGGTCAGACCCAGACGCGTGC CCCACCATGCCCCTGTTGGCCATGCTGCTCCGCGGGCTGACACAGATCCAGAGTCGGATCCTGGGCCCGG GGAGGAAGTGCTGTGCGCTGGCCAACCTGGCTGACATGCTGACTGTGTTTGCGCTGACAGAGGACGACCC CCAGGAGGTGTCTGCAACCGTGTATCTGGACAAACTGGCCACGGTGATCTCTGTGTGGAACTCGGACACC CAGAATCCCTACCACCAGCAGGCGCTGGCAGAGAAGGTGAAGGAGGCAGAACGGGATGTCAGCCTGACCT CGCTGGCCAAACTCCCCAGTGAGACCATTTTCGTGGGCTGCGAGTTCCTGCACCACCTGCTGCGGGAGTG GGGGGAGGAGTTGCAGGCCGTGCTCCGCAGCAGCCAGGGGACAAGTTACGACAGCTACCGGCTGTGCGAC AGTCTGACTTCCTTCAGCCAGAACGCGACGCTCTACCTGAACCGCACCAGCCTGTCCAAGGAGGACAGGC AGGTGGTCTCTGAGCTGGCGGAGTGTGTCAGGGACTTCCTGAGGAAAACGAGCACGGTGCTGAAGAACAG GGCCTTGGAGGATATCACAGCTTCCATTGCCATGGCCGTCATCCAGCAGAAGATGGACCGCCATATGGAA GTGTGCTACATTTTTGCCTCTGAGAAGAAGTGGGCCTTCTCGGACGAGTGGGTAGCCTGCCTGGGGAGTA ACAGGGCCCTCTTCCGAGAGCCAGACTTGGTGTTGAGGCTGCTGGAAACAGTGATAGACGTCAGCACAGC TGACAGAGCCATCCCTGAGTCTCAGATCCGGCAGGTGATCCACCTGATCCTGGAATGTTACGCAGACCTC TCCCTGCCAGGTAAAAATAAAGTCCTTGCAGGTATCCTGCGTTCCTGGGGGCGAAAGGGCCTCTCTGAAA AGTTGCTGGCTTATGTGGAGGGTTTTCAGGAAGACCTCAATACAACTTTTAACCAGCTCACTCAGAGTGC CTCCGAACAGGGCTTGGCAAAAGCTGTGGCCTCCGTGGCCCGCCTGGTCATAGTGCACCCGGAAGTCACG GTGAAGAAAATGTGCAGCCTGGCTGTGGTCAATCTCGGCACCCACAAGTTCCTGGGCCAGATTCTCACTG CCTTCCCTGCCCTTAGGTTTGTGGAAGAGCAGGGTCCCAATTCATCTGCCACTTTCATGGTGTCATGCCT CAAAGAAACCGTCTGGATGAAGTTCTCTACACCCAAGGAAGAAAAGCAATTTTTAGAGCTCCTGAACTGC CTGATGAGTCCCGTGAAACCCCAAGGGATTCCAGTGGCTGCTCTTCTTGAGCCAGACGAGGTGCTGAAGG AATTTGTCCTGCCTTTCTTGAGGTTAGATGTTGAAGAGGTAGACCTCAGTCTGAGGATCTTCATCCAGAC TCTAGAGGCAAACGCGTGCCGAGAGGAATACTGGCTCCAGACCTGCTCCCCGTTTCCACTCCTCTTCAGC TTGTGCCAGCTCTTGGACCGCTTCAGCAAATACTGGCAGCTTCCCAAGGAGAAGCGGTGCCTCTCTTTGG ATAGGAAGGATCTAGCGATCCATATCCTGGAGCTCCTGTGTGAGATTGTATCAGCCAATGCTGAGACCTT CTCCCCGGATGTCTGGATCAAGTCCCTGTCCTGGCTCCACCGCAAGTTAGAACAGCTAGACTGGACTGTG GGCCTGAGGCTGAAGAGCTTCTTCGAGGGGCACTTCAAGTGTGAAGTGCCAGCCACACTTTTTGAGATCT GTAAGCTTTCAGAAGACGAGTGGACCTCCCAGGCCCACCCAGGGTACGGGGCTGGCACGGGGCTCCTGGC CTGGATGGAGTGCTGCTGCGTCTCCAGCGGCATCTCGGAGAGGATGCTGTCTCTCTTGGTGGTGGACGTG GGCAATCCTGAGGAGGTCAGACTGTTCAGCAAAGGCTTTCTGGTGGCCCTGGTGCAAGTCATGCCTTGGT GCAGCCCTCAGGAGTGGCAGCGCCTTCACCAGCTGACCAGGAGACTGCTGGAGAAGCAGCTCCTCCATGT CCCTTATAGCCTGGAATATATTCAGTTTGTTCCCCTGCTCAACCTGAAGCCCTTTGCCCAGGAGTTGCAA CTCTCCGTCCTCTTCCTGAGGACTTTCCAGTTTCTCTGCAGCCATAGCTGTCGTAATTGGCTTCCTCTGG AAGGCTGGAACCACGTGGTCAAACTCCTCTGTGGCAGTCTGACCCGCCTCCTGGACTCAGTCAGGGCGAT ACAGGCAGCTGGCCCTTGGGTTCAAGGACCAGAGCAGGACCTGACCCAGGAAGCCCTGTTTGTTTACACC CAGGTGTTCTGCCATGCTCTGCACATCATGGCCATGCTCCACCCGGAGGTCTGTGAGCCACTCTACGTTT TAAGTATTGAAACCCTCACCTGCTATGAGACTTTGAGCAAGACCAACCCTTCTGTCAGCTCCTTGCGTTC AAGGGCACACGAGCAGCGCTTCTTAAAGTCCATTGCTGAGGGCATCGGCCCTGAAGAACGGCGCCAAACC CTGTTGCAGAAGATGAGCAGCTTC AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG203733 representing NM_015721
Red=Cloning site Green=Tags(s) MDLGPLNICEEMTILHGGFLLAEQLFHPKALAELTKSDWERVGRPIVEALREISSAAAHSQPFAWKKKAL IIIWAKVLQPHPVTPSDTETRWQEDLFFSVGNMIPTINHTILFELLKSLEASGLFIQLLMALPTTICHAE LERFLEHVTVDTSAEDVAFFLDVWWEVMKHKGHPQDPLLSQFSAMAHKYLPALDEFPHPPKRLRSDPDAC PTMPLLAMLLRGLTQIQSRILGPGRKCCALANLADMLTVFALTEDDPQEVSATVYLDKLATVISVWNSDT QNPYHQQALAEKVKEAERDVSLTSLAKLPSETIFVGCEFLHHLLREWGEELQAVLRSSQGTSYDSYRLCD SLTSFSQNATLYLNRTSLSKEDRQVVSELAECVRDFLRKTSTVLKNRALEDITASIAMAVIQQKMDRHME VCYIFASEKKWAFSDEWVACLGSNRALFREPDLVLRLLETVIDVSTADRAIPESQIRQVIHLILECYADL SLPGKNKVLAGILRSWGRKGLSEKLLAYVEGFQEDLNTTFNQLTQSASEQGLAKAVASVARLVIVHPEVT VKKMCSLAVVNLGTHKFLGQILTAFPALRFVEEQGPNSSATFMVSCLKETVWMKFSTPKEEKQFLELLNC LMSPVKPQGIPVAALLEPDEVLKEFVLPFLRLDVEEVDLSLRIFIQTLEANACREEYWLQTCSPFPLLFS LCQLLDRFSKYWQLPKEKRCLSLDRKDLAIHILELLCEIVSANAETFSPDVWIKSLSWLHRKLEQLDWTV GLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGAGTGLLAWMECCCVSSGISERMLSLLVVDV GNPEEVRLFSKGFLVALVQVMPWCSPQEWQRLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQ LSVLFLRTFQFLCSHSCRNWLPLEGWNHVVKLLCGSLTRLLDSVRAIQAAGPWVQGPEQDLTQEALFVYT QVFCHALHIMAMLHPEVCEPLYVLSIETLTCYETLSKTNPSVSSLRSRAHEQRFLKSIAEGIGPEERRQT LLQKMSSF SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015721 |
ORF Size | 3174 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015721.2, NP_056536.2 |
RefSeq Size | 3757 bp |
RefSeq ORF | 3177 bp |
Locus ID | 50628 |
UniProt ID | P57678 |
Protein Families | Druggable Genome |
Gene Summary | The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203733 | GEMIN4 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4) |
CNY 7,368.00 |
|
RC203733L3 | Lenti-ORF clone of GEMIN4 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4) |
CNY 12,450.00 |
|
RC203733L4 | Lenti-ORF clone of GEMIN4 (mGFP-tagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4) |
CNY 12,450.00 |
|
SC111260 | GEMIN4 (untagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4) |
CNY 7,376.00 |
|
SC323908 | GEMIN4 (untagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4) |
CNY 7,376.00 |
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