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ADPGK (NM_031284) Human Tagged ORF Clone
CAT＃: RG202965
- TrueORF®
ADPGK (tGFP-tagged) - Human ADP-dependent glucokinase (ADPGK), transcript variant 1
ORF Plasmid: DDK
"NM_031284" in other vectors (5)
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CNY 5,256.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610017G09Rik; ADP-GK |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG202965 representing NM_031284
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCTGTGGCGCGGCTCCGCGTACGCGGGCTTCCTGGCGCTGGCCGTGGGCTGCGTCTTCCTGCTGG AGCCAGAGCTGCCAGGCTCGGCGCTGCGCTCTCTCTGGAGCTCGCTGTGTCTGGGGCCCGCGCCTGCGCC CCCGGGACCCGTCTCCCCCGAGGGCCGGTTGGCGGCAGCCTGGGACGCGCTTATCGTGCGGCCAGTCCGG CGCTGGCGCCGCGTGGCAGTGGGAGTCAATGCATGTGTTGATGTGGTGCTCTCAGGGGTGAAGCTCTTGC AGGCACTTGGCCTTAGTCCTGGGAATGGGAAAGATCACAGCATTCTGCATTCAAGGAATGATCTGGAAGA AGCCTTCATTCACTTCATGGGGAAGGGAGCAGCTGCTGAGCGCTTCTTCAGTGATAAGGAAACTTTTCAC GACATTGCCCAGGTTGCGTCAGAGTTCCCAGGAGCCCAGCACTATGTAGGAGGAAATGCAGCTTTAATTG GACAGAAATTTGCAGCCAACTCAGATTTAAAGGTTCTTCTTTGCGGTCCAGTTGGTCCAAAGCTACATGA GCTTCTTGATGACAATGTCTTTGTTCCACCAGAGTCATTGCAGGAAGTGGATGAGTTCCACCTCATTTTA GAGTATCAAGCAGGGGAGGAGTGGGGCCAGTTAAAAGCTCCCCATGCCAACCGATTCATCTTCTCTCACG ACCTCTCCAACGGGGCCATGAATATGCTGGAGGTGTTTGTGTCTAGCCTGGAGGAGTTTCAGCCAGACCT GGTGGTCCTCTCTGGATTGCACATGATGGAGGGACAAAGCAAGGAGCTCCAGAGGAAGAGACTCTTGGAG GTTGTAACCTCCATTTCTGACATCCCCACTGGTATTCCAGTTCACCTAGAGCTGGCCAGTATGACTAACA GGGAGCTCATGAGCAGCATTGTCCATCAGCAGGTCTTTCCCGCGGTGACTTCCCTTGGGCTGAATGAACA GGAGCTGTTATTTCTCACCCAGTCAGCCTCTGGACCTCACTCTTCTCTCTCTTCCTGGAACGGTGTTCCT GATGTGGGCATGGTCAGTGACATCCTCTTCTGGATCTTGAAAGAACATGGGAGGAGTAAAAGCAGAGCCT CGGATCTCACCAGGATCCATTTCCACACGCTGGTCTACCACATCCTGGCAACTGTGGATGGACACTGGGC CAACCAGCTGGCAGCCGTGGCTGCAGGAGCTCGTGTGGCTGGGACACAGGCCTGCGCCACAGAAACCATA GACACCAGCCGAGTGTCTCTGAGGGCACCCCAAGAGTTCATGACTTCCCATTCGGAGGCAGGCTCCAGGA TTGTATTAAACCCAAACAAGCCAGTAGTAGAATGGCACAGAGAGGGAATATCCTTCCACTTCACACCAGT ATTGGTGTGTAAAGACCCCATTCGAACTGTAGGCCTTGGAGATGCCATTTCAGCCGAAGGACTCTTCTAT TCGGAAGTACACCCTCACTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG202965 representing NM_031284
Red=Cloning site Green=Tags(s) MALWRGSAYAGFLALAVGCVFLLEPELPGSALRSLWSSLCLGPAPAPPGPVSPEGRLAAAWDALIVRPVR RWRRVAVGVNACVDVVLSGVKLLQALGLSPGNGKDHSILHSRNDLEEAFIHFMGKGAAAERFFSDKETFH DIAQVASEFPGAQHYVGGNAALIGQKFAANSDLKVLLCGPVGPKLHELLDDNVFVPPESLQEVDEFHLIL EYQAGEEWGQLKAPHANRFIFSHDLSNGAMNMLEVFVSSLEEFQPDLVVLSGLHMMEGQSKELQRKRLLE VVTSISDIPTGIPVHLELASMTNRELMSSIVHQQVFPAVTSLGLNEQELLFLTQSASGPHSSLSSWNGVP DVGMVSDILFWILKEHGRSKSRASDLTRIHFHTLVYHILATVDGHWANQLAAVAAGARVAGTQACATETI DTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEWHREGISFHFTPVLVCKDPIRTVGLGDAISAEGLFY SEVHPHY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_031284 |
| ORF Size | 1491 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_031284.3, NP_112574.3 |
| RefSeq Size | 2492 bp |
| RefSeq ORF | 1491 bp |
| Locus ID | 83440 |
| UniProt ID | Q9BRR6 |
| Domains | ADP_PFK_GK |
| Gene Summary | ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis, possibly during ischemic conditions (Ronimus and Morgan, 2004 [PubMed 14975750]).[supplied by OMIM, Mar 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ADP-dependent glucokinase regulates energy metabolism via ER-localized glucose sensing
,Imle, R;Wang, BT;Stützenberger, N;Birkenhagen, J;Tandon, A;Carl, M;Himmelreich, N;Thiel, C;Gröne, HJ;Poschet, G;Völkers, M;Gülow, K;Schröder, A;Carillo, S;Mittermayr, S;Bones, J;Kamiński, MM;Kölker, S;Sauer, SW;,
Sci Rep
,PubMed ID 31582762
[ADPGK]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202965 | ADPGK (Myc-DDK-tagged)-Human ADP-dependent glucokinase (ADPGK), transcript variant 1 |
CNY 3,656.00 |
|
| RC202965L3 | Lenti ORF clone of Human ADP-dependent glucokinase (ADPGK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202965L4 | Lenti ORF clone of Human ADP-dependent glucokinase (ADPGK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC317832 | ADPGK (untagged)-Human ADP-dependent glucokinase (ADPGK), transcript variant 1 |
CNY 7,220.00 |
|
| SC319144 | ADPGK (untagged)-Human ADP-dependent glucokinase (ADPGK), transcript variant 1 |
CNY 3,656.00 |
